Advertisement

Intracellular Recordings from Neurones in Rat Cerebral Cortex During Hypoxia

  • J. Berg-Johnsen
  • Iver A. Langmoen
Conference paper
Part of the Acta Neurochirurgica book series (NEUROCHIRURGICA, volume 43)

Summary

Neurones in rat hippocampal cortex were exposed to hypoxia while their membrane properties and responses to different kinds of stimuli were recorded with an intracellular electrode. The initial changes consisted of a small depolarization followed by a hyperpolarization. Following these early events the neurones lost their membrane potential through a large depolarization. Similar changes were observed in neurones where the Na/K-ATPase was blocked by ouabain. Responses to direct application of the transmitters GABA and glutamate, which was lost at this point, were restored by passive reestablishment of the membrane potential with current through the intracellular electrode.

Keywords

Membrane Potential Input Resistance Intracellular Recording Brane Potential Hippocampal Pyramidal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Andersen P (1960) Interhippocampal impulses. II. Apical dendritic activation of CA 1 neurons. Acta Physiol Scand 48: 178–208Google Scholar
  2. 2.
    Andersen P, Dingledine R, Gjerstad L, Langmoen IA, Mosfeldt Laursen A (1980) Two different responses of hippocampal pyramidal cells to application of gamma-amino-butyric acid ( GABA ). J Physiol 305: 279–296PubMedCentralPubMedGoogle Scholar
  3. 3.
    Bosley TM, Woodhams PL, Gordon RD, Balazs R (1983) Effects of anoxia on the stimulated release of amino acid neurotransmitters in the cerebellum in vitro. J Neurochem 40: 189–201PubMedCrossRefGoogle Scholar
  4. 4.
    Dennis C, Kabat H (1939) Behaviour of dogs after complete temporary arrest of the cephalic circulation. Proc Soc Exp Biol Med 40: 559–581CrossRefGoogle Scholar
  5. 5.
    Grossman RG, Williams VF (1971) Electrical activity and ultrastructure of cortical neurons and synapses in ischaemia. In: Brierly JB, Meldrum BS (eds) Brain hypoxia. Heineman, London, pp 61–75Google Scholar
  6. 6.
    Haas HL, Schaerer B, Vosmansky M (1979) A simple perfusion chamber for the study of nervous tissue slices in vitro. J Neurosci Methods 1: 323–325PubMedCrossRefGoogle Scholar
  7. 7.
    Hablitz JJ, Langmoen IA (1982) Excitation of hippocampal pyramidal cells by glutamate in the guinea-pig and rat. J Physiol 325: 317–331PubMedCentralPubMedGoogle Scholar
  8. 8.
    Hansen AJ (1977) Extracellular potassium concentration in juvenile and adult rat brain cortex during anoxia. Acta Physiol Scand 99: 412–420PubMedCrossRefGoogle Scholar
  9. 9.
    Hansen AJ (1978) The extracellular potassium concentration in brain cortex following ischaemia in hypo-and hyperglycemic rats. Acta Physiol Scand 102: 324–329PubMedCrossRefGoogle Scholar
  10. 10.
    Hansen AJ (1985) Effect of anoxia on ion distribution in the brain. Physiol Rev 65: 101–148PubMedGoogle Scholar
  11. 11.
    Hansen AJ, Hounsgaard J, Jahnsen H (1982) Anoxia increases potassium conductance in hippocampal nerve cells. Acta Physiol Scand 115: 301–310PubMedCrossRefGoogle Scholar
  12. 12.
    Kolmodin GM, Skoglund CR (1959) Influence of asphyxia on membrane potential level and action potentials of spinal moto-and interneurons. Acta Physiol Scand 45: 1–18PubMedCrossRefGoogle Scholar
  13. 13.
    Langmoen IA, Andersen P (1981) The hippocampal slice in vitro. A description of the technique and some examples of the opportunities it offers. In: Kerkut GA, Wheal HV (eds) Electrophysiology of isolated mammalian CNS preparations. Academic Press, London, pp 55–105Google Scholar
  14. 14.
    Langmoen IA, Hablitz JJ (1981) Reversal potential for glutamate responses in hippocampal pyramidal cells. Neurosci Letters 23: 61–65CrossRefGoogle Scholar
  15. 15.
    Langmoen IA, Andersen P, Gjerstad L, Mosfeldt Laursen A, Ganes T (1978) Two separate effects of GABA on hippocampal pyramidal cells in vitro. Acta Physiol Scand 102: 28–29 AGoogle Scholar
  16. 16.
    Lipton P, Whittingham TS (1979) The effect of hypoxia on evoked potentials in the in vitro hippocampus. J Physiol 287: 427–438PubMedCentralPubMedGoogle Scholar
  17. 17.
    Rossen R, Kabat H, Anderson JP (1943) Acute arrest of cerebral circulation in man. Arch Neurol Psychiat (Chic) 50: 510–528CrossRefGoogle Scholar
  18. 18.
    Schiff SJ, Somjen GG (1985) Hyperexcitability following moderate hypoxia in hippocampal tissue slices. Brain Res 337: 337–340PubMedCrossRefGoogle Scholar
  19. 19.
    Schwartzkroin PA (1975) Characteristics of CA 1 neurons recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res 85: 423–436PubMedCrossRefGoogle Scholar
  20. 20.
    Schwartzkroin PA (1977) Further characteristics of hippocampal CA 1 cells in vitro. Brain Res 128: 53–68PubMedCrossRefGoogle Scholar
  21. 21.
    Schwartzkroin PA, Andersen P (1975) Glutamic acid sensitivity of dendrites in hippocampal slices in vitro. In: Kreutzberg GW (ed) Advances in neurology, vol 12. Raven Press, New York, pp 45–51Google Scholar
  22. 22.
    Speckmann E-J, Caspers H, Sokolov W (1970) Aktivitätsänderung spinaler Neurone während und nach einer Asphyxia. Pflügers Arch Ges Physiol 319: 122–138CrossRefGoogle Scholar
  23. 23.
    Williams V, Grossman RG (1970) Ultrastructure of cortical synapses after the failure of presynaptic activity in ischaemia. Anat Rec 166: 131–142PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • J. Berg-Johnsen
    • 1
    • 2
  • Iver A. Langmoen
    • 1
    • 2
  1. 1.Department of NeurosurgeryRikshospitalet, Institute for Experimental Medical Research, University of OsloNorway
  2. 2.Department of NeurosurgeryUllevål HospitalOsloNorway

Personalised recommendations