Summary
The weaver mutant mouse is characterized by degeneration of cerebellar granule cells in early post-natal stage. In the present study, the possibility of synapse formation in the weaver mouse cerebellum by implanted granule cells was analyzed immunohistochemically with antiserum against synaptic vesicle protein, Synapsin I. Normal cerebellar tissue, obtained from 15-day-old CBA/JNCij mouse embryos, was transplanted into the cerebellum of 4-week-old weaver mice. 6 weeks after the transplantation, the grafted tissue was clearly detected in the host cerebellum, developing a trilaminar organization. A number of granule-like cells were observed in the folia of the host cerebellum, suggesting that the implanted granule cells may have migrated from the grafted tissue into the host cerebellum. Some areas in the host cerebellum as well as in the grafted tissue were intensely stained by anti-Synapsin I serum, indicating that the implanted granule cells make synaptic contacts with the neuronal cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bloom FE, Ueda T, Battenberg E, Greengard P (1979) Immunocytochemical localization, in synapses, of protein I. An endogeneous substrate for protein kinases in mammalian brain. Proc Natl Acad Sci USA 76: 5982–5986
De Camilli P, Harris SM, Huttner WB, Greengard P (1983) Synapsin I (protein I), a nerve terminal-specific phosphoprotein. II. Its specific association with synaptic vesicles demonstrated by immunocytochemistry in agarose-embedded synaptosomes. J Cell Biol 96: 1355–1373
Dolphin AC, Greengard P (1981) Presence of protein I, a phosphoprotein associated with synaptic vesicles, in cerebellar granule cells. J Neurochem 36: 1627–1631
Inoue H, Kohsaka S, Otani M, Toya S, Tsukuda Y (1986) The effect of arcuate nucleus transplantation on the development of the anterior pituitary in monosodium glutamate-treated rats. Neurosci Res 3: 555–567
Inoue H, Kohsaka S, Yoshida K, Otani M, Toya S, Tsukada Y (1985) Cyclosporin A enhances the survivability of mouse cerebral cortex grafted into the third ventricle of rat brain. Neurosci Lett 54: 85–90
Inoue H, Kohsaka S, Yoshida K, Otani M, Toya S, Tsukada Y (1985) Immunohistochemical studies on mouse cerebral cortex grafted into the third ventricle of rats treated with cyclosporin A. Neurosci Lett 57: 289–294
Kohsaka S, Yoshida K, Inoue Y, Shinozaki T, Takayama H, Inoue H, Mikoshiba K, Takamatsu K, Otani M, Toya S, Tsukada Y (1986) Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice: immunohistochemical and electron microscopic studies on the myelination. Brain Res 372: 137–142
Rakic P, Sidman RL (1973) Sequence of developmental abnormalities leading to granule cell deficit in cerebellar cortex of weaver mutant mice. J Comp Neurol 152: 103–132
Rakic P, Sidman RL (1973) Organization of cerebellar cortex secondary to deficit of granule cells in weaver mutant mice. J Comp Neurol 152: 133–162
Rezai Z, Yoon CH (1972) Abnormal rate of granule cell migration in the cerebellum of “weaver” mutant mice. Dev Biol 29: 17–26
Sotelo C (1975) Anatomical, physiological and biochemical studies of the cerebellum from mutant mice. 2. Morphological study of cerebellar cortical neurons and circuits in the weaver mouse. Brain Res 94: 19–44
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody-enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complexes (horseradish-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18: 315–333
Ueda T, Maeno H, Greengard P (1973) Regulation of endogeneous phosphorylation of specific proteins in synaptic membrane fractions from rat brain by adenosine 3’: 5’-monophosphate. J Biol Chem 248: 8295–8305
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag
About this paper
Cite this paper
Takayama, H. et al. (1988). Possible Synapse Formation by Embryonic Cerebellar Tissue Grafted into the Cerebellum of the Weaver Mutant Mouse. In: Isamat, F., Jefferson, A., Loew, F., Symon, L. (eds) Proceedings of the 8th European Congress of Neurosurgery, Barcelona, September 6–11, 1987. Acta Neurochirurgica, vol 43. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8978-8_33
Download citation
DOI: https://doi.org/10.1007/978-3-7091-8978-8_33
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-8980-1
Online ISBN: 978-3-7091-8978-8
eBook Packages: Springer Book Archive