Expression of Cloned Influenza Virus Genes

  • Mary-Jane Gething
  • J. Sambrook


The cloning in bacterial plasmids of double-stranded DNA copies of the various RNA segments of the influenza virus genome has resulted in an unprecedented expansion of our knowledge about the proteins of the virus. Analysis of the DNA sequence of the cloned genes, added to the information available from earlier classical protein chemistry studies, has led to the elucidation of the amino acid sequences of all the known virus-coded proteins and to the discovery of hitherto unsuspected polypeptides; comparisons of the primary structures of the proteins coded by viruses of different subtypes has enhanced our understanding of the mechanisms of antigenic drift and shift; furthermore, knowledge of amino acid sequences has greatly simplified the interpretation of the three-dimensional structures of the external domains of the haemagglutinin (HA) and neuraminidase (NA) glycoproteins and allowed antigenic sites to be accurately mapped on the HA structure.


Influenza Virus Simian Cell Recombinant Genome Hybrid Transcript Radioimmune Assay 
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© Springer-Verlag/Wien 1983

Authors and Affiliations

  • Mary-Jane Gething
  • J. Sambrook

There are no affiliations available

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