Expression of Cloned Influenza Virus Genes
The cloning in bacterial plasmids of double-stranded DNA copies of the various RNA segments of the influenza virus genome has resulted in an unprecedented expansion of our knowledge about the proteins of the virus. Analysis of the DNA sequence of the cloned genes, added to the information available from earlier classical protein chemistry studies, has led to the elucidation of the amino acid sequences of all the known virus-coded proteins and to the discovery of hitherto unsuspected polypeptides; comparisons of the primary structures of the proteins coded by viruses of different subtypes has enhanced our understanding of the mechanisms of antigenic drift and shift; furthermore, knowledge of amino acid sequences has greatly simplified the interpretation of the three-dimensional structures of the external domains of the haemagglutinin (HA) and neuraminidase (NA) glycoproteins and allowed antigenic sites to be accurately mapped on the HA structure.
KeywordsInfluenza Virus Simian Cell Recombinant Genome Hybrid Transcript Radioimmune Assay
Unable to display preview. Download preview PDF.
- Gething, M.J., Sambrook, J.: The expression of the influenza vims haemagglutinin gene in SV40-HA recombinants. In: Eukaryotic Viral Vectors (Gluzman, Y., ed.), 29–33. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1982 a.Google Scholar
- Gluzman, Y. (ed.): Eukaryotic Viral Vectors. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1982.Google Scholar
- Maniatis, T., Fritsch, E. P., Sambrook, J.: Molecular Cloning-A Laboratory Manual. Cold Spring Harbor, N. Y: Cold Spring Harbor Laboratory 1982.Google Scholar
- McCutchan, J. H., Pagano, J. S.: Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethyl aminoethyl-dextran. J. Nafl. Cancer Inst. 41, 351–357 (1968).Google Scholar
- Mellon, P., Parker, V., Gluzman, Y., Maniatis, T.: Identification of DNA sequences required for transcription of the human β-globin gene in a new SV40 host-vector system. Cell 27, 279–288Google Scholar
- Neuberger, A., Gottschalk, A., Marshall, R. O., Spiro, R. G.: The Glycoproteins: Their Composition, Structure and Functions, 450–490. Amsterdam: Elsevier 1972.Google Scholar
- Pipas, J. M., Adler, S. P., Peden, K. W. C., Nathans, D.: Deletion mutants of SV40 that affect the structure of viral tumor antigens. Cold Spring Harbor Symp. Quant. Biol. 44, 285–291 (1979).Google Scholar
- Tooze, J. (ed.): DNA Tumor Viruses, 2nd ed. Cold Spring Harbor, N. Y.: Cold Spring Harbor Laboratory 1980.Google Scholar
- Young, J. F., Desselberger, U., Graves, P., Palese, P., Shatzman, A., Rosenberg, M.: Cloning and expression of influenza virus genes. In: The Origin of Pandemic Influenza Vimses (Laver, W. G., ed.). New York: Elsevier/North-Holland 1983 (in press).Google Scholar
- Zoller, M.J., Smith, M.: Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 derived vectors. Methods in Enzymology, in press (1983).Google Scholar