Isolation and Purification of Sialic Acids
The preparation of acylneuraminic acids is desirable to provide characterized standards for analytical methods and as a basis for the development of synthetic and new analytical techniques. Several methods exist for this, involving hydrolysis of material containing glycosidically bound sialic acids by dilute aqueous or methanolic acids or by enzymic means. Problems exist with respect to O-acyl-sialic acid preparation due to their lability during acid hydrolysis and their reduced or non-susceptibility towards the action of sialidases. Standard techniques have been developed over the last 15 years in the authors’ laboratory and these are discussed in the light of alternative methods and improvements which have appeared in the literature.
KeywordsSialic Acid Acid Hydrolysis Complex Carbohydrate Neuraminic Acid Total Sialic Acid
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