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Isolation and Purification of Sialic Acids

  • Roland Schauer
  • Anthony P. Corfield
Part of the Cell Biology Monographs book series (CELLBIOL, volume 10)

Abstract

The preparation of acylneuraminic acids is desirable to provide characterized standards for analytical methods and as a basis for the development of synthetic and new analytical techniques. Several methods exist for this, involving hydrolysis of material containing glycosidically bound sialic acids by dilute aqueous or methanolic acids or by enzymic means. Problems exist with respect to O-acyl-sialic acid preparation due to their lability during acid hydrolysis and their reduced or non-susceptibility towards the action of sialidases. Standard techniques have been developed over the last 15 years in the authors’ laboratory and these are discussed in the light of alternative methods and improvements which have appeared in the literature.

Keywords

Sialic Acid Acid Hydrolysis Complex Carbohydrate Neuraminic Acid Total Sialic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1982

Authors and Affiliations

  • Roland Schauer
    • 1
  • Anthony P. Corfield
    • 1
  1. 1.Biochemisches InstitutChristian-Albrechts-UniversitätKielFederal Republic of Germany

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