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Interdisciplinary Application of Modern Trace Analysis to Biologically Active Substances

  • Hans Georg Leemann
  • Fritz Erni
  • Bernhard Schreiber
Conference paper

Abstract

The power of detection and selectivity of modern analytical instruments are at present so high that trace analysis is used on a large scale in all areas of natural science.

By means of a few selected subjects, the possibilities and limitations of trace analysis characterization of biologically active substances are presented. Particular emphasis is laid on the preparation of samples, such as separation from the matrix, resolution of trace components and enrichment techniques. Selectivity and detection sensitivity are discussed, taking the determination of individual heavy metal trace elements in tissue as an example.

A closer look is taken at HPLC in the column switching mode combined with step gradient elution as one of the possibilities of separating trace organic compounds, in this case cyclosporin A, from an excess of other bio-organic material.

When physical chemical methods fail, the RIA test can be the method of choice as demonstrated in a methodological optimization process for a single dose migraine study with ergotamine.

What the trace analyst finds particularly interesting and attractive are the combination of methods. By means of an example, the yes-no presence of LSD in human blood, a multiple combination of RIA, HPLC and fluorescence spectroscopy shows how difficult analytical problems can be solved in an elegant manner by skillful, sequential combination of separation and measurement steps.

Also a review of the most recent technical literature is provided.

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography Trace Analysis Ergot Alkaloid Column Switching 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    H. Sigel, Metal Ions in Biological Systems, Marcel Dekker Inc. New York, Basel, (1978)Google Scholar
  2. 2.
    H. Aebi, Z. Praeventivmed. 1, 137–149 (1956)CrossRefGoogle Scholar
  3. 3.
    Wissenschaftliche Tabellen GEIGY, 7. Auflage, 228–241 (1965)Google Scholar
  4. 4.
    E. Baumgartner, Mtt. Geb. Lebensm. Hyg. 70, 34–42, (1979)Google Scholar
  5. 5.
    H.A. Schroeder, Trace Elements and Nutrition, Faber and Faber, London (1976)Google Scholar
  6. 6.
    F. Kieffer, Sandoz Bulletin 51, 52, 53 (1980), Sandoz AG, BaselGoogle Scholar
  7. 7.
    J.R. McDermott, A.I. Smith, K. Iqbal and H.M. Wisniewski, Lancet Oct.1, 710 (1977)CrossRefGoogle Scholar
  8. 8.
    W.J. Weiner, P.A. Nausieda and H.L. Klawans, Neurology 28, 734 (1978)Google Scholar
  9. 9.
    H.R. Linder, H.D. Seltner and B. Schreiber, Anal. Chem. 50, 896 (1978)CrossRefGoogle Scholar
  10. 10.
    D.M. Hercules, ed. Detectors for Trace Organic Analysis by Liquid Chromatography: Principles and Applications in: Contemporary Topics in Analytical and Clinical Chemistry, Vol.2, Plenum Publishing Corp., 1978, New York.Google Scholar
  11. 11.
    Symposium on Drug Analysis in Biological Material-Principle Aspects, Stockholm, Nov. 1–3, 1978.Google Scholar
  12. 12.
    W.J. Serfontein, D. Botha and L. de Villiers, J. Pharm. Pharmac. 27, 937, 973 (1975)CrossRefGoogle Scholar
  13. 13.
    W. Dünges, Prä-chromatographische Mikromethoden R.E. Kaiser ed. Dr.Alfred Hüthig Verlag, Heidelberg, Basel, New York, 1979Google Scholar
  14. 14.
    I.S. Forrest, A.G. Bolt and M.T. Serra, Life Science 5, 473 (1966)CrossRefGoogle Scholar
  15. 15.
    S.R. Lipsky, W.J. McMurray, M. Hermandez, J.E. Purcell and K.A. Billeb, J. Chromatogr. Sci. 18, 1 (1980)Google Scholar
  16. 16.
    M.L. Lee and B.W. Wright, J. Chromatogr. 184, 235 (1980)CrossRefGoogle Scholar
  17. 17.
    W.H. McFadden, J. Chromatogr. Sci. 17, 1 (1979)Google Scholar
  18. 18.
    F. Erni and A. Melera, J. Chromatogr. in press.Google Scholar
  19. 19.
    A. Melera and F. Erni, J. Chromatogr. in press.Google Scholar
  20. 20.
    W.H. McFadden, J. Chromatogr. Sci. 18, 97 (1980)Google Scholar
  21. 21.
    A. RUegger, M. Kuhn, H. Lichti, H.R. Loosli, R. Huguenin, C. Quiquerez and A. von Wartburg, Helv. Chim. Acta 59, 1075 (1976).CrossRefGoogle Scholar
  22. 22.
    J.F. Lawrence, J. Chromatogr. Sci. 17, 113 (1979)Google Scholar
  23. 23.
    J.C. Gfeller, Ä.K. Beck and D. Seebach, Helv. Chim. Acta 63, 728 (1980)CrossRefGoogle Scholar
  24. 24.
    W. Niederberger, P. Schaub and T. Beveridge, J. Chromatogr. 182, 454 (1980), Biomed. Applic.CrossRefGoogle Scholar
  25. 25.
    F. Erni, H.P. Keller, C. Morin and M. Schmitt, J. Chromatogr. in press.Google Scholar
  26. 26.
    F. Erni and R.W. Frei, J. Chromatogr. 130, 169 (1977)CrossRefGoogle Scholar
  27. 27.
    F. Erni and R.W. Frei, J. Chromatogr. 149, 561 (1978)CrossRefGoogle Scholar
  28. 28.
    S.A. Berson and R.S. Yalow, Clin. Chim. Acta 22, 51 (1968)CrossRefGoogle Scholar
  29. 29.
    S.A. Berson and R.S. Yalow, Radioimmunoassay: A Status Report (1971) in: Immunobiology, Sinnauer Associates Inc., Stanford, Con. 06905.Google Scholar
  30. 30.
    S. Spector, Ann. Rev. Pharmacol., 13, 359 (1973)CrossRefGoogle Scholar
  31. 31.
    B.A. Peskar and B.M. Peskar, Eur. J. Drug Metab. Pharmacokinet., 4, 163 (1977)CrossRefGoogle Scholar
  32. 32.
    G.P. Mould, G.W. Aherne, B.A. Monis, J.D. Teale and V. Marks, Eur. J. Drug Metab. Pharmacokinet., 4, 171 (1977)CrossRefGoogle Scholar
  33. 33.
    W. M. Hunter, Handbook of Experimental Immunology, Vol. 1 (Immuno-chemistry), Blackwell Scientific Publications, Oxford, (1978)Google Scholar
  34. 34.
    R. Augstin, Handbook of Experimental Immunology, Vol. 3 (Application of mmunological Methods) Blackwell Scientific Publications, Oxford, (1978)Google Scholar
  35. 35.
    B.F. Erlanger, Pharmacol. Rev. 25, 271 (1973)Google Scholar
  36. 36.
    V.P. Butler, J. Immunol. Methods, 7, 1 (1975)CrossRefGoogle Scholar
  37. 37.
    K. Lubke and B. Nieuwboer, Immunologische Teste für niedermolekulare Wirk stoffe, Georg Thieme Verlag, Stuttgart (1978)Google Scholar
  38. 38.
    H. Eckert, J.R. Kiechel, J. Rosenthaler, R. Schmidt, E. Schreier in:Ergot Alkaloids and Related Compounds, B. Berde, H.O. Schild Eds., Springer-Verlag, Berlin (1978), p. 767Google Scholar
  39. 39.
    P.I. Twitchett, S.M. Fletcher, A.I. Sullivan and A.C. Moffat, J. Chromato gr. 150, 73 (1978)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1981

Authors and Affiliations

  • Hans Georg Leemann
    • 1
  • Fritz Erni
    • 1
  • Bernhard Schreiber
    • 1
  1. 1.Analytical Research and Development, Pharmaceutical DivisionSANDOZ Ltd.BasleSwitzerland

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