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Microscale Analysis of Biomolecules by Time Resolved Laser Spectroscopy

  • Rudolf Rigler
Conference paper

Abstract

Principles and applications of techniques for the study of dynamic properties of biomolecules in the time range 10−12 — l02 sec are presented. They are based on the spectroscopic properties of laser radiation permitting extreme time and spectral resolution as well as sensitivity of detection. In this context instruments were developed in our laboratory for the measurement of fluorescence anisotropy decay, fluorescence correlations, fluorescence photobleaching recovery as well as dynamic laser light scattering. From the analysis of these spectroscopic properties information about the dynamics of the molecular architecture and the solution structure of biomolecules as well as on their interactions can be obtained.

Keywords

Autocorrelation Function Rotational Diffusion Fluorescence Correlation Spectroscopy Translational Diffusion Autocorrelation Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1981

Authors and Affiliations

  • Rudolf Rigler
    • 1
  1. 1.Department of Medical BiophysicsKarolinska InstitutetStockholm 60Sweden

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