Organization and Replication of the Genome

  • Samuel Dales
  • Beatriz G. T. Pogo
Part of the Virology Monographs book series (VIROLOGY, volume 18)


As might be anticipated from the complexity of their architecture and polypeptide content, the poxviruses contain a large molecular weight genome. By direct visualization after spreading on protein films by the Kleinschmidt procedure or by physical-chemical determinations, it is evident that the genomes exist as double stranded linear DNA molecules about 45 to 60 µm long, (Fig. 11 A) (Randall et al., 1966; Hyde et al., 1967; Gafford and Randall, 1967; Gafford et al., 1978; Arif, 1976; Geshelin and Berns, 1974). A presumed detection of single stranded DNA within the virion (Pfau and McCrea, 1962a, b) has not been substantiated and this result could, conceivably, have been obtained artifactually as a consequence of the extraction method employed, which produces fragments of denatured DNA.


Protein Film Molluscum Contagiosum Inverted Terminal Repeat Sequence Alkaline Sucrose Gradient Poxvirus Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag/Wien 1981

Authors and Affiliations

  • Samuel Dales
    • 1
  • Beatriz G. T. Pogo
    • 2
  1. 1.Cytobiology Group, Department of Microbiology and Immunology, Faculty of Medicine, Health Sciences CentreUniversity of Western OntarioLondonCanada
  2. 2.Departments of Experimental Cell Biology and MicrobiologyMount Sinai School of Medicine, The Mount Sinai HospitalNew YorkUSA

Personalised recommendations