Morphogenesis of Tracheary Elements and Guard Cells

  • P. K. Hepler
Part of the Cell Biology Monographs book series (CELLBIOL, volume 8)


Plant cell shape is determined by the cell wall. The pattern and orientation of the reinforcing cellulose microfibrils of the wall define the manner and places in which a cell will strain in response to the internally generated turgor stress (Green 1980). To understand morphogenesis one must explain the mechanism of cell wall formation and in particular how unique patterns of cellulose reinforcements arise. Two cell types that possess specific wall patterns related to cell shape and function, and that have received considerable attention in studies of cytomorphogenesis are tracheary elements and stomatal guard cells (Hepler and Palevitz 1974). Despite their obvious differences in overall shape and function they possess remarkable similarities in the structure of their secondary wall and in the apparent manner by which the wall develops. It is the purpose of this chapter to consider the mechanism of secondary wall formation in differentiating tracheary elements and guard cells and to focus especially on those structures and processes at the cell wall-cyto- plasmic interface that seems to be responsible for Controlling the location of wall formation and the orientation of cellulose deposition.


Guard Cell Secondary Wall Cellulose Microfibril Tracheary Element Primary Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Allen, R. D., Bajer, A., la Fountain, J., 1969: Poleward migration of particles or states in spindle fiber filaments during mitosis in Haemanthus. J. Cell Biol. 43, 4 a.Google Scholar
  2. Bloodgood, R. A., 1977: Motility occurring in association with the surface of the Chlamy- domonas flagellum. J. Cell Biol. 75, 983–989.PubMedCrossRefGoogle Scholar
  3. Brower, D. L., Hepler, P. K., 1976: Microtubules and secondary wall deposition in xylem: the effects of isopropyl N-phenylcarbamate. Protoplasma 87, 91–111.PubMedCrossRefGoogle Scholar
  4. Cronshaw, J., Bouck, G. B., 1965: The fine structure of differentiating xylem elements. J. Cell Biol. 24, 415–431.PubMedCrossRefGoogle Scholar
  5. Dentler, W. L., 1981: Microtubule-membrane interactions in cilia and flagella. Inter. Rev. Cytol. 72, 1–47.CrossRefGoogle Scholar
  6. — Pratt, M. M., Stephens, R. E., 1980: Microtubule-membrane interactions in cilia. II. Photochemical cross-linking of bridge structures and the identification of a membrane- associated dynein-like ATPase. J. Cell Biol. 84, 381–403.PubMedCrossRefGoogle Scholar
  7. Edelman, G., 1976: Surface modulation in cell recognition and cell growth. Science 192, 218–226.PubMedCrossRefGoogle Scholar
  8. Esau, K., 1953: Plant anatomy, p. 735. New York: J. Wiley.Google Scholar
  9. — Cheadle, V. I., Gill, R. H., 1966 a: Cytology of differentiating tracheary elements. I. Organelles and membrane systems. Amer. J. Bot. 53, 756–764.CrossRefGoogle Scholar
  10. — — — 1966 b: Cytology of differentiating tracheary elements. II. Structures associated with cell surfaces. Amer. J. Bot. 53, 765–771.CrossRefGoogle Scholar
  11. Giddings, T. H., jr., Brower, D. L., Staehelin, L. A., 1980: Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls. J. Cell Biol. 84, 327–339.PubMedCrossRefGoogle Scholar
  12. Goosen-De Roo, L., 1973 a: The relationship between cell organelles and cell wall thickenings in primary tracheary elements of the cucumber. II. Quantitative aspects. Acta Bot. Neerl. 22, 301–320.Google Scholar
  13. — 1973 b: The fine structure of the protoplast in primary tracheary elements of the cucumber after plasmolysis. Acta Bot. Neerl. 22, 467–485.Google Scholar
  14. Green, P. B., 1980: Organogenesis—a biophysical view. Ann. Rev. Plant Physiol. 31, 51–82.CrossRefGoogle Scholar
  15. Gunning, B. E. S., Hardham, A. R., Hughes, J. E., 1978 a: Preprophase bands of microtubules in all categories of formative and proliferative cell division in Azolla roots. Planta 143, 145–160.CrossRefGoogle Scholar
  16. — — — 1978 b: Evidence for initiation of microtubules in discrete regions of the cell cortex in Azolla root-tip cells, and an hypothesis on the development of cortical arrays of microtubules. Planta 143, 161–179.CrossRefGoogle Scholar
  17. Hardham, A. R., Gunning, B. E. S., 1978: Structure of cortical microtubule arrays in plant i cells. J. Cell Biol. 77, 14–34.PubMedCrossRefGoogle Scholar
  18. — — 1979: Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots of Azolla pinnata. J. Cell Sci. 37, 411–442.PubMedGoogle Scholar
  19. — — 1980: Some effects of colchicine on microtubules and cell division in roots of Azolla pinnata. Protoplasma 102, 31–51.CrossRefGoogle Scholar
  20. Heath, I. B., 1974: A unified hypothesis for the role of membrane bound enzyme complexes and microtubules in plant cell wall synthesis. J. Theor. Biol. 48, 445–449.PubMedCrossRefGoogle Scholar
  21. Hepler, P. K., Fosket, D. E., 1971: The role of microtubules in vessel member differentiation in Coleus. Protoplasma 72, 213–236.CrossRefGoogle Scholar
  22. — — Newcomb, E. H., 1970: Lignification during secondary wall formation in Coleus: an electron microscopic study. Amer. J. Bot. 57, 85–96.CrossRefGoogle Scholar
  23. — Newcomb, E. H., 1963: The fine structure of young tracheary xylem elements arising by redifferentiation of parenehyma in wounded Coleus stem. J. Exp. Bot. 14, 496–503.CrossRefGoogle Scholar
  24. — — 1964: Microtubules and fibrils in the cytoplasm of Coleus cells undergoing secondary wall deposition. J. Cell Biol. 20, 529–533.PubMedCrossRefGoogle Scholar
  25. Hepler, P. K., Palevitz, B. A., 1974: Microtubules and microfilaments. Ann. Rev. Plant Physiol. 25, 309–362.CrossRefGoogle Scholar
  26. — Rice, R. M., Terranova, W. A., 1972: Cytochemical localization of peroxidase activity in wound vessel members of Coleus. Can. J. Bot. 50, 977–983.CrossRefGoogle Scholar
  27. Kaufman, P. B., Petering, L. B., Yocum, C. S., Baic, D., 1970: Ultrastructural studies on stomatal development in internodes of Avena sativa. Amer. J. Bot. 57, 33–49.CrossRefGoogle Scholar
  28. Kiermayer, O., Sleytr, U. B., 1979: Hexagonally ordered “rosettes” of particles in the plasma membrane of Micrasterias denticulata Breb. and their significance for microfibril formation and orientation. Protoplasma 101, 133–138.CrossRefGoogle Scholar
  29. Ledbetter, M., Porter, K. R., 1963: A “microtubule” in plant cell fine structure. J. Cell Biol. 19, 239–250.PubMedCrossRefGoogle Scholar
  30. Lin, D. C., Tobin, K. D., Grumet, M., Lin, S., 1980: Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation. J. Cell Biol. 84, 455–460.PubMedCrossRefGoogle Scholar
  31. Lloyd, C. W., Slabas, A. R., Powell, A. J., Lowe, S. B., 1980: Microtubules, protoplasts and plant cell shape. Planta 147, 500–506.CrossRefGoogle Scholar
  32. Maitra, S. C., De, D. N., 1971: Role of microtubules in secondary thickening of differentiating xylem element. J. Ultrastruct. Res. 34, 15–22.PubMedCrossRefGoogle Scholar
  33. Mueller, S. C., Brown, R. M., jr., 1980: Evidence for an intramembrane component associated with a cellulose microfibril-synthesizing complex in higher plants. J. Cell Biol. 84, 315–326.PubMedCrossRefGoogle Scholar
  34. Northcote, D. H., 1969: Fine structure of cytoplasm in relation to synthesis and secretion in plant cells. Proc. Roy. Soc. B 173, 21–30.Google Scholar
  35. Palevitz, B. A., 1978: Cortical microtubules in plant cells: a high voltage electron microscope (HVEM) study. J. Cell Biol. 79, 278 a.Google Scholar
  36. — 1980 a: Comparative effects of phalloidin and cytochalasin B on motility and morphogenesis in Allium. Can. J. Bot. 58, 773–785.CrossRefGoogle Scholar
  37. — 1980 b: The structure and development of stomatal cells. In: Stomatal physiology (Jarvis, P. G., Mansfield, T. A., eds.). Soc. Exp. Biol. Sem. Ser. Cambridge University Press, Cambridge, U.K.Google Scholar
  38. — Hepler, P. K., 1974 a: The control of the plane of division during stomatal differentiation in Allium. I. Spindle reorientation. Chromosoma 46, 297–326.Google Scholar
  39. — — 1974 b: The control of the plane of division during stomatal differentiation in Allium. II. Drug studies. Chromosoma 46, 327–341.CrossRefGoogle Scholar
  40. — — 1976: Cellulose microfibril orientation and cell shaping in developing guard cells of Allium: the role of microtubules and ion accumulation. Planta 132, 71—93.CrossRefGoogle Scholar
  41. Pickett-Heaps, J. D., 1967 a: The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation, and distribution of cytoplasmic microtubules. Dev. Biol. 15, 206–236.CrossRefGoogle Scholar
  42. — 1967 b: Ultrastructure and differentiation in Chara sp. I. Vegetative cells. Aust. J. Biol. Sci. 20, 539–551.Google Scholar
  43. — 1968: Xylem wall deposition. Radioautographic investigations using lignin precursors. Protoplasma 65, 181–205.CrossRefGoogle Scholar
  44. — 1969: Preprophase microtubule bands in some abnormal mitotic cells of wheat. J. Cell Sci. 4, 397–420.PubMedGoogle Scholar
  45. — Northcote, D. H., 1966 a: Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J. Cell Sci. 1, 109–120.PubMedGoogle Scholar
  46. — — 1966 b: Cell division in the formation of the stomatal complex of the young leaves of wheat. J. Cell Sci. 1, 121–128.PubMedGoogle Scholar
  47. — — 1966 c: Relationship of cellular organelles to the formation and development of the plant cell wall. J. Exp. Bot. 17, 20–26.CrossRefGoogle Scholar
  48. Quader, H., Wagenbreth, I., Robinson, D. G., 1978: Structure, synthesis and orientation of microfibrils. V. On the recovery of Oocystis solitaria from microtubule inhibitor treatment. Cytobiologie 18, 39–51.PubMedGoogle Scholar
  49. Raschke, K., 1979: Movements of stomata. In: Physiology of movements (Haupt, W., Feinleib, M. E., eds.), pp. 383–441. (Encycl. Plant Physiol., New Series, Vol. 7.) Berlin- Heidelberg-New York: Springer.Google Scholar
  50. Schnabl, H., Vienken, J., Zimmerman, U., 1980: Regulär arrays of intramembranous particles in the plasmalemma of guard cell and mesophyll cell protoplasts of Vicia faba. Planta 148, 231–237.CrossRefGoogle Scholar
  51. Singh, A. P., Srivastava, L. M., 1973: The fine structure of pea stomata. Protoplasma 76, 61–82.CrossRefGoogle Scholar
  52. Sinnott, E. W., Bloch, R., 1945: The cytoplasmic basis of intracellular patterns in vascular differentiation. Amer. J. Bot. 32, 151–157.CrossRefGoogle Scholar
  53. Srivastava, L. M., Singh, A. P., 1972 a: Stomatal structure in com leaves. J. Ultrastruct. Res. 39, 345–363.PubMedCrossRefGoogle Scholar
  54. — — 1972 b: Certain aspects of xylem differentiation in com. Can. J. Bot. 50, 1795–1804.CrossRefGoogle Scholar
  55. Wieland, T., 1977: Modification of actin by phallotoxins. Naturwiss. 64, 303–309.PubMedCrossRefGoogle Scholar
  56. Wooding, F. B. P., Northcote, D. H., 1964: The development of the secondary wall of the xylem in Acer pseudoplatanus. J. Cell Biol. 23, 327—337.PubMedCrossRefGoogle Scholar
  57. Zeiger, E., 1971: Cell kinetics, development of stomata and some effects of colchicine in barley. Planta 99, 89–111.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1981

Authors and Affiliations

  • P. K. Hepler
    • 1
  1. 1.Botany DepartmentUniversity of MassachusettsAmherstUSA

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