Metabolic Alterations in an Animal Model of Huntington’s Disease Using the 14C-Deoxyglucose Method

  • H. Kimura
  • Edith G. McGeer
  • P. L. McGeer
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 16)


Various brain regions showing altered glucose uptake in an animal model of Huntington’s disease (HD) were identified by the 14C-2-deoxy- glucose (DG) autoradiographic technique. Rats with kainic acid (KA) lesions of the neostriatum were used as an animal model of HD. KA-injected animals showed reduced utilization of DG in the injected neostriatum as well as in the ipsilateral rostral sulcal cortex, dentate fascia of hippocampus, ventromedial nucleus of the thalamus and cortico-bulbar tract. By contrast, enhanced uptake was found in the ipsilateral globus pallidus, entopeduneular nucleus, the area lateral to the lateral hypothalamus, the lateral habenular nucleus and pars reticulata of the substantia nigra. The results provide interesting in vivo metabolic and functional information on brain circuits involved in motor Performance.


Kainic Acid Medial Forebrain Bundle Ventromedial Nucleus Intrastriatal Injection Deoxyglucose Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, M., Yoshida, M.: Electrophysiological evidence for branching nigral projections to the thalamus and the superior collicus. Brain Research 137, ([0-9]+)–([0-9]+) (1977).PubMedCrossRefGoogle Scholar
  2. Burger, B., Thierry, A. M., Tassin, J. P., Moyne, M. A.: Dopaminergic innervation of the rat prefrontal cortex: A fluorescence histochemical study. Brain Research 106 133–145(1976).CrossRefGoogle Scholar
  3. Coyle, J.T., McGeer, E. G, McGeer, P. L., Schwartz,Neostriatal injections: A model for Huntington’s chorea. In: Kainic Acid as a Tool in Neurobiology (McGeer, E. G., Olney, J. W., McGeer, P. L., eds.), pp. 139–160. New York: Raven Press. 1978.Google Scholar
  4. Fibiger, H. C.: Kainic acid lesions of the striatum: A pharmacological and behavioral model of Huntington’s disease. In: Kainic Acid as a Tool in Neurobiology (McGeer, E. G., Olney, J. W., McGeer, P. L., eds.), pp. 161–176. New York: Raven Press. 1978.Google Scholar
  5. Gottesfeld, Z., Kvetnansky, R., Kopin, I. J., Jacobowitz, D. M.: Effects of repeated immobilization stress on glutamate decarboxylase and choline acetyltransferase in discrete brain regions. Brain Research 152, 374–378 (1978).PubMedCrossRefGoogle Scholar
  6. Jakubovic, A., Lin, D., McGeer, E. G.: Protein and RNA synthesis in kainic acid-injected striata. Brain Research 163, 289–294 (1979).PubMedCrossRefGoogle Scholar
  7. Kanazawa, I., Toyokura, Y.: Topographical study of the distribution of gamma-aminobutyric acid (GABA) in the human substantia nigra. A case study. Brain Research 100, 371–381 (1975).PubMedCrossRefGoogle Scholar
  8. Kimura, H., Wada, J. A.: Metabolic exploration of amygdaloid (AM) kindled seizure using 14C-2-deoxyglucose (DG) method. Am. Epil. Soc. Abstract, 1979.Google Scholar
  9. König, J.F. R., Klippel, R. A.: The rat brain, a stereotaxic atlas of the forebrain and lower parts of the brain stem. Huntington, N.Y.: 1963.Google Scholar
  10. McGeer, E. G., Meer, P. L.: Some factors influencing the neurotoxicity of intrastriatal injections of kainic acid. Neurochem. Res. 3, 501–517 (1978).PubMedCrossRefGoogle Scholar
  11. Nadler, J. V., Vaca K. W., Frost White, W., Lynch, G. S., Cotman, C. W.: Aspartate and glutamate as possible transmitters of excitatory hippocampal afferents. Nature 260, 538–540 (1976).PubMedCrossRefGoogle Scholar
  12. Schwartz, W. J., Sharp, F. R., Gunn, R.H., Evarts, E. V.: Lesions of ascending dopaminergic pathways decrease forebrain glucose uptake. Nature 261, 155–157 (1976).PubMedCrossRefGoogle Scholar
  13. Sokoloff, L. Reivich, M., Kennedy, C., Des Rosiers, M. H., Patlak, C. S., Pettigrew, D., Sukurada, O., Shinohara, H.: The [l4C] deoxyglucose method for the measurement of local cerebral glucose utilization: theory procedure and normal values in the conscious and unconscious albino rat. J. Neurochem. 28, 897–916 (1977).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • H. Kimura
    • 1
  • Edith G. McGeer
    • 1
  • P. L. McGeer
    • 1
    • 2
  1. 1.Kinsmen Laboratory of Neurological Research, Department of PsyciiatryUniversity of British ColumbiaVancouverCanada
  2. 2.Laboratory of Neurological Research University of British ColumbiaUniversity of British ColumbiaVancouverCanada

Personalised recommendations