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Limitations on the Transposition of TnA

  • Conference paper
R-Factors: Their Properties and Possible Control

Part of the book series: Topics in Infectious Diseases ((TIDIS,volume 2))

Abstract

In the relatively short space of time since the process of transposition has been defined with some precision (4, 13, 14, 15, 19, 22, 23), the number of transposons known to occur in Gramnegative bacteria has increased dramatically. The initial experiments were all concerned with the ampicillin transposon (TnA, now redesignated Tn1, Tn2, Tn3 — see ref.7), but subsequently units which specify the transposition of trimethoprim and streptomycin resistance (Tn7) (2, 7), tetracycline resistance (Tn10) (7, 11, 18), neomycin and kanamycin resistance (Tn5) (5, 7), chloramphenicol resistance (Tn9) (7, 12), and mercury ion resistance (Tn501) (24) have all been characterised with a varying degree of completeness. In all cases in which the phenomenon of transposition has been investigated in sufficient detail, it is known that the process occurs independently of the host’s classical recombination systems (that is, the process will occur in recA bacteria (2, 4, 11, 12, 14, 18, 19, 22, 24) and results in the acquisition of a discrete piece of DNA by the recipient replicon (2, 4, 5, 12, 14, 18, 19, 22, 23, 24). Thus transposition of TnA normally results in an increase in size of the recipient plasmid of a piece of DNA of molecular weight about 3.2 Mdal.

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Authors
  1. P. M. Bennett
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  2. M. K. Robinson
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  3. M. H. Richmond
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Editor information

Editors and Affiliations

  1. Section of Chemotherapy, Sandoz Forschungsinstitut Gesellschaft m. b. H., Brunnerstrasse 59, A-1235, Vienna, Austria

    Jürgen Drews (Head) (Head)

  2. Department of Experimental Chemotherapy, Sandoz Forschungsinstitut Ges. m. b. H., Brunnerstrasse 59, A-1235, Vienna, Austria

    Gregor Högenauer

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© 1977 Springer-Verlag/Wien

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Bennett, P.M., Robinson, M.K., Richmond, M.H. (1977). Limitations on the Transposition of TnA. In: Drews, J., Högenauer, G. (eds) R-Factors: Their Properties and Possible Control. Topics in Infectious Diseases, vol 2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8501-8_5

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  • DOI: https://doi.org/10.1007/978-3-7091-8501-8_5

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-8503-2

  • Online ISBN: 978-3-7091-8501-8

  • eBook Packages: Springer Book Archive

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