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Plasmid-Mediated Tetracycline Resistance in E. coli

  • Stuart B. Levy
  • Laura McMurry
  • Philip Onigman
  • Richard M. Saunders
Part of the Topics in Infectious Diseases book series (TIDIS, volume 2)

Abstract

The tetracyclines are effective bacteriostatic drugs for a wide variety of microbial infections. They inhibit the binding of aminoacyl transfer RNA to the 30S ribosomal subunit (1). More specifically they appear to interfere with the codon-anticodon interaction (2). The drug has worldwide usage therapeutically as well as prophylactically in humans, domestic and farm animals, and plants. It is, therefore, not surprising that the emergence of resistance to this drug has also occurred worldwide and in epidemic proportions. In fact, in most organisms tested, if resistances to antibiotics have been found, tetracycline resistance is among them. The list includes all Enterobacteriaceae, Pseudomonas, Hemophilus influenza, Streptococcus fecalis, Clostridia perfringins and Staphylococcus aureus (3,4). The most common mode of resistance is by plasmids on which are found the genes for resistance. A clear understanding of the mechanism of resistance in these different species has not yet been reached. Some information has been obtained, however, in studies of Enterobacteriaceae (5,6,7) and Staphylococcus aureus (8,9). In most Enterobacteriaceae studied and in Staphylococcus expression of resistance is regulated (6,7,9). We have identified a tetracycline-inducible protein, TET, associated with tetracycline resistance (7,10) in E. coli and we have partially purified a “repressor” which regulates synthesis of this protein (11). Our results suggest that, as is TET protein, so is the whole tetracycline resistance operon under negative control releasable by tetracycline.

Keywords

Resistant Cell Sensitive Cell Uptake System Tetracycline Resistance Internal Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Suzuki, I., Kaji, H. and Kaji, A.: Binding of specific sRNA to 30s ribosomal subunits: effects of 50s ribosomal subunits. Proc. Nat. Acad. Sci., USA 55: 1483–1490, 1966.CrossRefGoogle Scholar
  2. 2.
    Högenauer, G. and Turnowsky, F.: The effects of streptomycin and tetracycline on codon-anticodon interactions. FEBS Lett. 26: 185–188, 1972.PubMedCrossRefGoogle Scholar
  3. 3.
    Falkow, S.: Infectious Multiple Drug Resistance. Pion. Limited, 1975.Google Scholar
  4. 4.
    Davies, J. and Novick, R. (edit.): Proceedings of the 2nd ASM Conference on Extrachromosomal Elements. Microbiology, 1977.Google Scholar
  5. 5.
    Izaki, K., Kiuchi, K. and Arima, K.: Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bact. 91: 628–633, 1966.PubMedGoogle Scholar
  6. 6.
    Franklin, T.J.: Resistance to Escherichia coli to tetracycline. Changes in permeability to tetracyclines in Escherichia coli bearing transferable resistance factors. Biochem. J. 105: 371–378, 1967.PubMedGoogle Scholar
  7. 7.
    Levy, S.B. and McMurry, L.: Detection of an inducible membrane protein associated with R factor mediated tetracycline resistance. Biochem. Biophys. Res. Comm. 56: 1060–1068, 1974.PubMedCrossRefGoogle Scholar
  8. 8.
    Sompolinsky, D., Krawitz, T., Zaidenzaig, Y. and Abramova, N.: Inducible resistance to tetracycline in Staphylococcus aureus. J. Gen. Microbial. 62: 341–349, 1970.Google Scholar
  9. 9.
    Sompolinsky, D., Zaidenzaig, Y., Ziegler-Schlomowitz, R. and Abramova, N.: Mechanism of tetracycline resistance in Staphylococcus aureus. J. Gen. Microbial. 62: 351–362, 1970.Google Scholar
  10. 10.
    Levy, S.B.: The relation of a tetracycline-induced R factor membrane protein to tetracycline resistance, in Drug-Inactivating Enzymes and Antibiotic Resistances. (Editors: Mitsuhashi, S., Rosival, L., Kréméry, V.) Berlin, Springer-Verlag, 1975.Google Scholar
  11. 11.
    Yang, H-L., Zubay, G. and Levy, S.B.: Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. Proc. Nat. Acad. Sci., USA 73: 1509–1512, 1976.CrossRefGoogle Scholar
  12. 12.
    Shipley, P.L. and Olsen, R.H.: Characteristics and expression of tetracycline resistance in gram negative bacteria carrying the Pseudomonas R factor RP1. Antimicrob. Agents and Chem. 6: 183–188, 1974.Google Scholar
  13. 13.
    Unowsky, J. and Rachmeler, M.: Mechanisms of antibiotic resistance determined by resistance transfer factors. J. Bact. 92: 358–365, 1966.PubMedGoogle Scholar
  14. 14.
    Reynard, A.M., Nellis, L.F. and Beck, M.E.: Uptake of 3H-tetracycline by resistant and sensitive Escherichia coli. Applied Microbiol. 21: 71–75, 1971.Google Scholar
  15. 15.
    Del Bene, V.E. and Rogers, M.: Comparison of tetracycline and mina -cycline transport in Escherichia coli. Antimicrob. Agents and Chem. 7: 801–806, 1975.Google Scholar
  16. 16.
    Levy, S.B. and McMurry, L.: Probing the expression of plasmid-mediated tetracycline resistance in E. coli. Microbiology, 1977 (in press).Google Scholar
  17. 17.
    DeZeeuw, J.R.: Accumulation of tetracyclines by Escherichia coli. J. Bact. 95: 498–506, 1968.Google Scholar
  18. 18.
    Heppel, L.A.: The concept of periplasmic enzymes in Structure and Function of Biological Membranes.(Editor: Rothfield, L.I.) Academic Press, New York and London, pp. 223–247, 1971.Google Scholar
  19. 19.
    Levy, S.B.: R factor proteins synthesized in Escherichia coli minicells: Incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins. J. Bact. 120: 1451–1463, 1974.PubMedGoogle Scholar
  20. 20.
    Novashin, S.M., Beliayskaya, I.V., Sazykin, Y.O. and Gryaznora, N.S.: Tetracycline resistance unassociated with a change of cell wall permeability in Escherichia coli in Drug-Inactivating Enzymes and Antibiotic Resistances. (Editors: Mitsuhashi, S., Rosival, L., Krcméry, V.) Berlin, Springer-Verlag, pp. 227–230, 1975.CrossRefGoogle Scholar
  21. 21.
    Arima, K. and Izaki, K.: Accumulation of oxytetracycline relevant to its bacterial action in the cells of Escherichia coli. Nature 200: 192–193, 1963.PubMedCrossRefGoogle Scholar
  22. 22.
    Franklin, T.J. and Godfrey, A.: Resistance of Escherichia coli to tetracyclines. Biochem. J. 94: 54–60, 1965.PubMedGoogle Scholar
  23. 23.
    Levy, S.B. and McMurry, L.: Plasmid-mediated tetracycline resistance involves an alternative transport system for tetracycline. (To be submitted.)Google Scholar
  24. 24.
    Klein, W.L. and Boyer, P.D.: Energization of active transport by Escherichia coli. J. Biol. Chem. 247: 7257–7265, 1972.PubMedGoogle Scholar
  25. 25.
    Lehninger, A.L.: Biochemistry. North Pub., New York, p. 429, 1975.Google Scholar
  26. 26.
    Cox, G.B. and Gibson, F.: Studies on electron transport and energy-linked reactions using mutants of Escherichia coli. B.B.A. 346: 1–25, 1974.Google Scholar
  27. 27.
    Franklin, T.J. and Foster, S.J.: Expression of R factor-mediated resistance to tetracycline in Escherichia coli minicells. Antimicrob. Agents and Chem. 5: 194–195, 1974.Google Scholar
  28. 28.
    Levy, S.B., McMurry, L. and Palmer, E.: R factor proteins synthesized in Escherichia coli minicells. II. Membrane-associated R factor proteins. J. Bact. 120: 1464–1471, 1974.PubMedGoogle Scholar
  29. 29.
    Foster, T.J.: Tetracycline-sensitive mutants of the F-like R factors R100 and R100–1. Molec. Gen. Genet. 137: 85–88, 1975.PubMedGoogle Scholar
  30. 30.
    Reeve, E.C.R. and Robertson, J.M.: The characteristics of eleven mutants of R factor R57 constitutive for tetracycline resistance, selected and tested in Escherichia coli K12. Genet. Res. Cant. 2: 297–312, 1975.CrossRefGoogle Scholar
  31. 31.
    Starlinger, P. and Saedler, H.: Insertion elements in micro-organisms in Current Topics in Microbiology and Immunology 75: 111, 1976.PubMedGoogle Scholar
  32. 32.
    Blumberg, D.D. and Malamy, M.H.: Evidence for the presence of non-translated T7 late mRNA in infected F’ (PIF+) episome-containing cells. J. Virol. 13: 378–385, 1975.Google Scholar
  33. 33.
    Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1: 190–206, 1955.PubMedCrossRefGoogle Scholar
  34. 34.
    Osborn, M.J., Gander, J.E., Parisi, E. and Carson, J.: Mechanism of assembly of outer membrane of Salmonella typhimurium: isolation and characterization of cytoplasmic and outer membrane. J. Biol. Chem. 247: 3962–3972, 1972.PubMedGoogle Scholar
  35. 35.
    Bonner, W.N. and Laskey, R.A.: A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46: 83–88, 1974.PubMedCrossRefGoogle Scholar
  36. 36.
    Studier, F.W.: Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J. Mol. Biol. 79: 237–248, 1973.PubMedCrossRefGoogle Scholar
  37. 37.
    Levy, S.B.: Very stable prokaryotic messenger RNA in chromosomeless Escherichia coli minicells. Proc. Nat. Acad. Sci. USA 72: 2900–2904, 1975.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1977

Authors and Affiliations

  • Stuart B. Levy
  • Laura McMurry
  • Philip Onigman
  • Richard M. Saunders

There are no affiliations available

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