Elimination of Plasmidic Determinants by DNA-Complexing Compounds
Solutions to problems posed by R-factor determined multiresistance of pathogenic bacteria to chemotherapeutic drugs might be approached by several avenues. Firstly, one might search for new chemotherapeutic drugs. Secondly, one could think of interfering with the formation of R-factor gene products through inhibition of the transcription of messenger RNA or of its translation into proteins. In instances in which new drugs had these modes of action, the two first approaches would become unitary. Thirdly, one might hope to forestall the recombinatorial formation of new R-factors. Fourthly, one could think of inhibiting the transmission of R-factors, and, lastly, one might attempt to eliminate R-factors by selective inhibition of R-factor DNA replication. To the extent to which this replication is required for recombination of plasmidic genes into new R-factors or for transmission, the last three approaches would be served jointly by effective inhibition of plasmidic DNA replication. This is the approach which we have taken.
KeywordsMethylene Blue Nalidixic Acid Acridine Orange Resistance Determinant Phenethyl Alcohol
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