Spin-Labelled Intermediates as Targets of Antibiotic Action in Peptidoglycan Synthesis

  • L. S. Johnston
  • W. P. Hammes
  • H. A. Lazar
  • F. C. Neuhaus
Conference paper
Part of the Topics in Infectious Diseases book series (TIDIS, volume 1)


Spin labels provide sensitive probes to study the microenvironments of intermediates in membrane catalyzed reactions. The electron spin resonance (ESR) spectrum of the spin label is a function of the motion that the probe experiences and the polarity of the solvent surrounding the probe. Thus, information about the mobility of the spin label and its microenvironment can be deduced from its spectrum. Indeed, McConnell and other investigators have utilized this technique to analyze lateral diffusion of phospholipids, conformation changes, flip-flop of phospholipids, phase transitions, and fluidity in membranes. It is the purpose of this paper to report some of our observations on spin-labeled intermediates in cell wall biosynthesis and how they may be used as targets of antibiotic action.


Electron Spin Resonance Electron Spin Resonance Spectrum Spin Label Rotational Correlation Time Peptidoglycan Synthesis 
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Copyright information

© Springer-Verlag/Wien 1975

Authors and Affiliations

  • L. S. Johnston
  • W. P. Hammes
  • H. A. Lazar
  • F. C. Neuhaus

There are no affiliations available

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