Spin-Labelled Intermediates as Targets of Antibiotic Action in Peptidoglycan Synthesis
Spin labels provide sensitive probes to study the microenvironments of intermediates in membrane catalyzed reactions. The electron spin resonance (ESR) spectrum of the spin label is a function of the motion that the probe experiences and the polarity of the solvent surrounding the probe. Thus, information about the mobility of the spin label and its microenvironment can be deduced from its spectrum. Indeed, McConnell and other investigators have utilized this technique to analyze lateral diffusion of phospholipids, conformation changes, flip-flop of phospholipids, phase transitions, and fluidity in membranes. It is the purpose of this paper to report some of our observations on spin-labeled intermediates in cell wall biosynthesis and how they may be used as targets of antibiotic action.
KeywordsElectron Spin Resonance Electron Spin Resonance Spectrum Spin Label Rotational Correlation Time Peptidoglycan Synthesis
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- Ghuysen, J. -M. and G. D. Shockman: Biosynthesis of peptidoglycan, In L. Leive (Editor), Bacterial Membranes and Walls, vol. 1, p. 37–130. Marcel Dekker, Inc., New York (1973).Google Scholar
- Hammes, W. P. and F. C. Neuhaus: Biosynthesis of peptidoglycan in Gaffkya homari: role of the peptide subunit of UDP-MurNAc-pentapeptide. J. Bacteriol. in press.Google Scholar
- Hammes, W. P. and F. C. Neuhaus: On the mechanism of action of vancomycin: inhibition of peptidoglycan synthesis in Gaffkya homari. Submitted for publication. Antimicrobial Agents and Chemotherapy.Google Scholar
- Johnston, L. S. and F. C. Neuhaus, unpublished observations (1974).Google Scholar
- Jost, P. and O. H. Griffith: Electron spin resonance and the spin labeling method, In C. Chignell (Editor), Methods in Pharmacology, vol. 2, p. 223–276. Appleton-Century-Crofts, New York (1972).Google Scholar
- Jordan, D. C. and P. E. Reynolds: Vancomycin, In D. Gottlieb and P. D. Shaw (Editors), Antibiotics: mechanism of action, vol. 1, p. 102–116. Springer-Verlag, Heidelberg (1967).Google Scholar
- Swenson, J. C.: Isolation, characterization, and possible function of a new peptidoglycan precursor [UDP-N-acetylmuramyl-alanyl-y-D-glutamyl-lysyl(e-alanyl)-D-alanyl-D-alanine]Irom Staphylococcus aureus Copenhagen, Ph.D. thesis, Northwestern University (1974).Google Scholar