Experiments on the Binding Sites and the Action of Some Antibiotics which Inhibit Ribosomal Functions

  • R. Werner
  • A. Kollak
  • D. Nierhaus
  • G. Schreiner
  • K. H. Nierhaus
Part of the Topics in Infectious Diseases book series (TIDIS, volume 1)


We describe experiments (A) on an antibiotic which binds to the 50S subunit of procaryotic ribosomes (chloramphenicol), (B) on an antibiotic which binds specifically to the 30S subunit (streptomycin), and (C) on a group of antibiotics whose binding sites are not yet well known (tetracycline and some of its derivatives).


Ribosomal Subunit Equilibrium Dialysis Peptidyltransferase Center Dependent Binding Ribosomal Activity 
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  1. BISWAS, D.K., and L. GORINI: The attachment site of streptomycin to the 30S ribosomal subunit. Proc. Nat. Acad. Sci. USA 69, 2141–2144 (1972).PubMedCrossRefGoogle Scholar
  2. BODLEY, J.N., and F.J. ZIEVE: On the specificity of the two ribosomal binding sites: studies with tetracycline. Biochem. Biophys. Res. Commun. 36, 463–468 (1969).PubMedCrossRefGoogle Scholar
  3. CAPECCHI, M.R., and H.A. KLEIN: Characterization of three proteins involved in polypeptide chain termination. Cold Spring Harbor Symp. Quant. Biol. 34, 469–491 (1969).Google Scholar
  4. CELMA, M.L., R.F. MONRO, and D. VAZQUEZ: Substrate and antibiotic binding sites at the peptidyltransferase center of E. coli ribosomes: Binding of UACCA-Leu to 50S subunits. FEBS Lett. 13, 247251 (1971).Google Scholar
  5. CERNA, J., I. RYCHLIK, and P. PULKRABEK: The effect of antibiotics on the coded binding of peptidyl-tRNA to the ribosome and on the transfer of the peptidylresidue to puromycin. Eur. J. Biochem. 9, 27–35 (1969).PubMedCrossRefGoogle Scholar
  6. DAY, L.E.: Tetracycline inhibition of cell-free protein synthesis. J. Bacteriol. 92, 197–203 (1966).PubMedGoogle Scholar
  7. DIETRICH, S., I. SCHRANDT, and K.H. NIERHAUS: Interdependence of E. coli ribosomal proteins at the peptidyltransferase center. FEBS Letters, in press.Google Scholar
  8. FERNANDEZ-MUNOZ, R., R.E. MONRO, R. TORRES-PINEDO, and D. VAZQUEZ: Substrate-and antibiotic binding sites of the peptidyltransferase center of Escherichia coli ribosomes. Eur. J. Biochem. 23, 185–193 (1971a).CrossRefGoogle Scholar
  9. FERNANDEZ-MUNOZ, R., R.E. MONRO, and D. VAZQUEZ: Ribosomal peptidyltransferase: Binding of inhibitors. Methods in Enzymology (Academic Press, New York and London), Vol. XX, part C, 481–490 (1971b).Google Scholar
  10. GOTTESMAN, M.E.: Reaction of ribosome-bound peptidyl transfer ribonucleic acid or puromycin. J. Biol. Chem. 242, 5564–5571 (1967).PubMedGoogle Scholar
  11. HIEROWSKI, M.: Inhibition of protein synthesis by chlortetracycline in the Escherichia coli in vitro system. Proc. Nat. Acad. Sci. USA 53, 594–599 (1965).PubMedCrossRefGoogle Scholar
  12. HOMANN, H.E., and K.H. NIERHAUS: Protein compositions of biosynthetic precursors and artificial subparticles from ribosomal subunits in Escherichia coli K12. Eur. J. Biochem. 20, 249–257 (1971).PubMedCrossRefGoogle Scholar
  13. LESSARD, J.L., and PESTKA, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. CCIII. Chloramphenicol, aminoacyl oligonucleotides, and Escherichia coli ribosomes. J. Biol. Chem. 247, 6909–6912 (1972).PubMedGoogle Scholar
  14. MAXWELL, J.H.: Studies of the binding of tetracycline to ribosomes in vitro. Mol. Pharmacol. 4, 25–37 (1968).PubMedGoogle Scholar
  15. MIZUSHIMA, S., and M. NOMURA: Assembly mapping of 30S ribosomal proteins from E. coli. Nature (London) 226, 1214–1218 (1970).CrossRefGoogle Scholar
  16. NIERHAUS, K.H., and V. MONTEJO: A protein involved in the peptidyltransferase activity of Escherichia coli ribosomes. Proc. Nat. Acad. Sci. USA 70, 1931–1935 (1973).PubMedCrossRefGoogle Scholar
  17. NIERHAUS, D., and K.H. NIERHAUS: Identification of the chloramphenicol-binding protein in Escherichia coli ribosomes by partial reconstitution. Proc. Nat. Acad. Sci. USA 70, 2224–2228 (1973).PubMedCrossRefGoogle Scholar
  18. PESTKA, S.: Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenyl alanyl-oligonucleotide binding to ribosomes. Proc. Nat. Acad. Sci. USA 64, 709–714 (1969).PubMedCrossRefGoogle Scholar
  19. ROTH, H.E., and K.H. NIERHAUS: Isolation of four ribonucleoprotein fragments from the 30S subunit of E. coli ribosomes. FEES Lett. 31, 35–38 (1973).CrossRefGoogle Scholar
  20. SCHREINER, G., and K.H. NIERHAUS: Protein involved in the binding of dihydrostreptomycin to ribosomes of Escherichia coli. J. Mol. Biol. 81, 71–82 (1973).PubMedCrossRefGoogle Scholar
  21. SUAREZ, G., and D. NATHANS: Inhibition of aminoacyl-sRNA binding to ribosomes by tetracycline. Biochem. Biophys. Res. Commun. 18, 743–750 (1965).CrossRefGoogle Scholar
  22. VOGEL, Z., A. ZAMIR, and D. ELSON: The possible involvement of peptidyltransferase in the termination step of protein biosynthesis. Biochemistry 8, 5161 (1969).PubMedCrossRefGoogle Scholar
  23. WATANABE, S.: Interaction of siomycin with the acceptor site of Escherichia coli ribosomes. J. Mol. Biol. 67, 443–457 (1972).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1975

Authors and Affiliations

  • R. Werner
  • A. Kollak
  • D. Nierhaus
  • G. Schreiner
  • K. H. Nierhaus

There are no affiliations available

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