Experiments on the Binding Sites and the Action of Some Antibiotics which Inhibit Ribosomal Functions
We describe experiments (A) on an antibiotic which binds to the 50S subunit of procaryotic ribosomes (chloramphenicol), (B) on an antibiotic which binds specifically to the 30S subunit (streptomycin), and (C) on a group of antibiotics whose binding sites are not yet well known (tetracycline and some of its derivatives).
KeywordsRibosomal Subunit Equilibrium Dialysis Peptidyltransferase Center Dependent Binding Ribosomal Activity
Unable to display preview. Download preview PDF.
- CAPECCHI, M.R., and H.A. KLEIN: Characterization of three proteins involved in polypeptide chain termination. Cold Spring Harbor Symp. Quant. Biol. 34, 469–491 (1969).Google Scholar
- CELMA, M.L., R.F. MONRO, and D. VAZQUEZ: Substrate and antibiotic binding sites at the peptidyltransferase center of E. coli ribosomes: Binding of UACCA-Leu to 50S subunits. FEBS Lett. 13, 247251 (1971).Google Scholar
- DIETRICH, S., I. SCHRANDT, and K.H. NIERHAUS: Interdependence of E. coli ribosomal proteins at the peptidyltransferase center. FEBS Letters, in press.Google Scholar
- FERNANDEZ-MUNOZ, R., R.E. MONRO, and D. VAZQUEZ: Ribosomal peptidyltransferase: Binding of inhibitors. Methods in Enzymology (Academic Press, New York and London), Vol. XX, part C, 481–490 (1971b).Google Scholar