The Mitogenic Activity of Lipopolysaccharides on Lymphocytes in Culture

I. Blastogenic Activity of Lipopolysaccharide in Mouse Lymphocytes Depleted of Antigen Reactive Cells
  • C. Damais
  • C. Bona
  • C. Galanos
  • L. Chedid


Lipopolysaccharides (LPS) from gram-negative bacteria have been found to be strong mitogens for mouse spleen lymphocytes (1) but induce only a weak stimulation of human blood (1, 2), guinea pig (3) and rabbit lymphocytes (2, 4, 5). In chicken the LPS failed to activate bursa cells (B1) but transformed a small proportion of bursa-derived cells (B2) compared to the strong stimulation of these populations by anti-chicken Ig serum (6).


Spleen Lymphocyte Germ Free Mouse Blast Transformation Mouse Lymphocyte Scintillation Method 
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  1. 1.
    Peavy, D. L., Adler, W. H., Smith, R. T.: J. Immunol. 105: 1453 (1970).PubMedGoogle Scholar
  2. 2.
    Bona, C., Damais, C., Dimitriu, A., Chedid, L., Ciorbaru, R., Adam, A., Petit, J. F., Lederer, E., Rosselet, J. P.: Mitogenic effect of a water soluble extract of Nocardia opaca. A comparative study with some bacterial adjuvants on spleen and peripheral lymphocytes of four mammalian species. J. Immunol. (in press)Google Scholar
  3. 3.
    Bona, C.: La pinocytose de toxines bactériennes par les macrophages et les implications immunologiques (Ph. D. thesis). Arch. Biol. 82: 323 (1971).Google Scholar
  4. 4.
    Oppenheim, J. J., Perry, S.: Proc. Soc. exp. Biol. Med. 118: 1014 (1965).Google Scholar
  5. 5.
    Sell, S., Sheppard, H. W.: Science 182: 586 (1973).PubMedCrossRefGoogle Scholar
  6. 6.
    Weber, W. T.: J. Immunol. 111: 1277 (1973).PubMedGoogle Scholar
  7. 7.
    Janossy, G., Humphrey, J. H., Greaves, M. F.: Bacterial lipopolysaccharide stimulation of a subpopulation of B lymphocytes due to lipid A but not to complement activation, (Manuscript sent for consultation by J. H. Humphrey).Google Scholar
  8. 8.
    Andersson, J., Melchers, F., Galanos, C., Lüderitz, O.: J. exp. Med. 137: 943 (1973).PubMedCrossRefGoogle Scholar
  9. 9.
    Coutinho, A., Moller, G.: Nature New Biol. 245: 12 (1973).PubMedGoogle Scholar
  10. 10.
    Bona, C., Damais, C., Chedid, L.: Blastic transformation of mice spleen lymphocytes by a water soluble mitogen extracted from Nocardia. Proc. nat. Acad. Sci. (in press).Google Scholar
  11. 11.
    Boivin, A., Mesrobeanu, L.: Rev. Immunol. 1: 553 (1935).Google Scholar
  12. 12.
    Westphal, O., Lüderitz, O., Bister, F.: Z. Naturforsch. 7b: 148 (1952).Google Scholar
  13. 13.
    Galanos, C., Rietschel, E. T., Lüderitz, O., Westphal, O.: Europ. J. Biochem. 19: 143 (1971).PubMedCrossRefGoogle Scholar
  14. 14.
    Bona, C., Dumitrescu, S.M., Heuclin, C., Pariset, C.: Recherches concernant le developpement ontogénique des antigènes de cytodifférenciation lymphocytaire chez le poussin et leur controle génétique chez la souris. Dans Etude phylogénique et ontogénique de la réponse immune, INSERM 1972, 229.Google Scholar
  15. 15.
    Landy, M., Sanderson, R. P., Jackson, A. L.: J. exp. Med. 122: 483 (1965).PubMedCrossRefGoogle Scholar
  16. 16.
    Bona, C., Trebiciaysky, I., Anteunis, A., Heuclin, C., Robineaux, R.: Europ. J. Imm. 2: 434 (1972).CrossRefGoogle Scholar
  17. 17.
    Peavy, D. L., Shands, J. W., Adler, W. A., Smith, R. T.: J. Immunol. 111: 352 (1973).PubMedGoogle Scholar
  18. 18.
    Cohen, J. J., Claman, H. N.: J. exp. Med. 133: 1026 (1971).PubMedCrossRefGoogle Scholar
  19. 19.
    Playfair, J. H. L.: Clin. exp. Immunol. 8: 839 (1971).PubMedGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1975

Authors and Affiliations

  • C. Damais
  • C. Bona
  • C. Galanos
  • L. Chedid

There are no affiliations available

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