Abstract
In studying toxicity we have demonstrated (1, 2) that the purified endotoxins extracted by the method of Boivin (3) or by sodium chloride-sodium citrate hypertonic solution (4) are more toxic than those obtained by the corresponding lipopolysaccharide phenol water procedure of Westphal et al. (5) or by the technique of Galanos (6). According to some authors, lipid A solubilized with bovine serum albumin exhibits equal endotoxic activity as the original LPS in lethal toxicity or pyrogenicity (Lüderitz et al., Intern. Symp. on toxic products of bacteria, Prague, September 1973). According to other authors, the content of lipid A from LPS is not related to the level of toxicity (D. Mlynarcik, same symposium). To try to solve this problem, we have previously proved (1, 2) that a Triton X100 treatment could reveal an antigenic subfraction enriched in nitrogen from the endotoxin extracted by the trichloracetic method of Boivin (3) or by the sodium chloride-sodium citrate procedure (4).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Chermann, J. - C., Digeon, M., Kouznetzova, B., Raynaud, M. Symp, Seriesimmunobiol. Standard. 15: 123 (1971).
Raynaud, M., Kouznetzova, B., Navarro, M. - J., Chermann, J. M., Petitprez, A.: J. infect. Dis., Supplement 128: 35 (1973).
Boivin, A., Mesrobeanu, I., Mesrobeanu, L.: C. R. Soc. Biol. (1933).
Raynaud, M., Digeon, M.: C. R. Acad. Sci. Paris 229: 564 (1949).
Westphal, O., Lüderitz, O., Bister, F.: Z. Naturforsch. 7 b: 148 (1952).
Galanos, C., Lüderitz, O., Westphal, O.: Europ. J. Biochem. 9: 245 (1969).
Mendiola, L. R., Kouznetzova, B., Chermann, J. - C., Sinoussi, F., Digeon, M., Raynaud, M.: Infection and Immunity 6: 27 (1972).
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1975 Springer-Verlag/Wien
About this chapter
Cite this chapter
Raynaud, M., Chermann, JC., Navarro, MJ., Kouznetzova, B. (1975). Purification of PO, Antigenic Subfraction of the Endotoxin Extracted from S. typhi O 901. In: Urbaschek, B., Urbaschek, R., Neter, E. (eds) Gram-Negative Bacterial Infections and Mode of Endotoxin Actions. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8396-0_15
Download citation
DOI: https://doi.org/10.1007/978-3-7091-8396-0_15
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-8398-4
Online ISBN: 978-3-7091-8396-0
eBook Packages: Springer Book Archive