Species Differences in the Effectiveness of Intestinal Barriers against Penetration of Inert Particulates and Bacteria

  • H. Cottier
  • Th. Schaffner
  • A. D. Chanana
  • D. D. Joel
  • B. Sordat
  • B. J. Bryant
  • M. W. Hess


Host and microflora interactions occur on all surfaces of the body exposed to the environment. The microbial ecology of the gastrointestinal tract may be regarded as the most important site of interaction if numbers of microorganisms and diversity and magnitude of antigenic stimulation involved are considered. Because of suppression of, or competition with, potential invaders, possibly also by virtue of its ability to block access of invaders to the tissues, the autochthonous flora contributes to the defense of the host. It may also be related to the mosaic-like distribution of bacterial species along the gastrointestinal tract. It has been recognized in recent years that intimate symbiotic associations between microorganisms and the inner surface of the gastrointestinal tract in small rodents are instrumental in maintaining a state of health. An unexpected finding was that large numbers of hitherto unknown, highly oxygen-sensitive bacteria form dense layers covering the mucosal epithelium (1, 2, 3). Little is known with respect to the relative importance of secretory IgA, lysozyme, complement components and other humoral factors for defense against microorganisms in the neighbourhood of, or within, intestinal epithelial layers. A so-called chemical barrier resembling the intracellular digestive apparatus, as observed in mammalian tissue culture cells (4), has been proposed to exist near the brush border of epithelial cells where activities of enzymes such as acid phosphatase and nonspecific esterase have been demonstrated. However, this defense mechanism does not provide sufficient protection against such pathogenic microbes as Salmonella, Shigella, Cryptosporidia and other organisms which penetrate and/ or reside and multiply within the brush border or the entire epithelial layer (review: 5); moreover, it is sensitive to the action of certain enterotoxins such as those from E. coli and Vibrio cholerae (6, 7, 8).


Brush Border Mesenteric Lymph Node Epithelial Layer Nonspecific Esterase Cell BioI 
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  1. 1.
    Savage, D. C., Dubos, R., Schaedler, R. W. J. exp. Med. 127: 67 (1968).Google Scholar
  2. 2.
    Lee, A., Gordon, J., Dubos, R.: Nature 220: 1137 (1968).PubMedCrossRefGoogle Scholar
  3. 3.
    Savage, D. C., McAllister, J. S.: Microbial interactions at body surfaces andresistance to infectious diseases. In: Dunlop, R. H., Moon, H. W.: Resistance to Infectious Disease, p.113 (Saskatoon Modern Press 1970 ).Google Scholar
  4. 4.
    Gordon, G. B., Miller, L. R., Bensch, K. G.: J. Cell Biol. 25: 41 (1965).PubMedCrossRefGoogle Scholar
  5. 5.
    Takeuchi, A.: Current Topics in Pathology 54: 1 (1971).Google Scholar
  6. 6.
    Love, A. H. G.: Gut 10: 105 (1969).CrossRefGoogle Scholar
  7. 7.
    Moon, H. W., Whipp, S. C., Baetz, A. L.: Lab. Invest. 25: 133 (1971).PubMedGoogle Scholar
  8. 8.
    Yardley, J. H., Brown, G. D.: Lab. Invest. 28: 482 (1973).PubMedGoogle Scholar
  9. 9.
    Gordon, L. E., Ruml, D., Hahne, H. J., Miller, C. Ph.: J. exp. Med. 102: 413 (1955).PubMedCrossRefGoogle Scholar
  10. 10.
    Thomas, H. C., McSween, R. N. M., White, R. G.: Lancet I: 1288 (1973).Google Scholar
  11. 11.
    Hammond, C, W., Tompkins, M., Miller, C. Ph.: J. exp. Med. 99: 405 (1954).Google Scholar
  12. 12.
    Hammond, C. W., Colling, M., Cooper, D. B., Miller, C. Ph.: J. exp. Med. 99: 411 (1954).PubMedCrossRefGoogle Scholar
  13. 13.
    Cottier, H.: Histopathologie der Wirkung ionisierender Strahlen auf höhere Organismen (Tier und Mensch). In: Diethelm, L., Olsson, O., Strnad, F., Vie-ten, H., Zuppinger, A.: Handbuch der Medizinischen Radiologie, p. 3, Bd. II/2 ( Springer, Berlin-Heidelberg-New York 1966 ).Google Scholar
  14. 14.
    Herbst, E. F. G.: Das Lymphgefäßsystem und seine Verrichtungen ( Vandenhoeck und Ruprecht, Göttingen 1844 ).Google Scholar
  15. 15.
    Mason, J. H., Dalling, T., Gordon, W. S.: J. Path. Bact. 33: 783 (1930).CrossRefGoogle Scholar
  16. 16.
    Aschaffenburg, R., Bartlett, S., Kon, S. K., Roy, J. H. B., Walker, D., Briggs, C., Lovell, R.: Brit. J. Nutr. 5: 171 (1951).CrossRefGoogle Scholar
  17. 17.
    Bangham, D. R., Ingram, P. L., Roy, J. H. B., Shillam, K. W. G., Terry, R. J.: Proc. Roy. Soc. B 149: 184 (1958).CrossRefGoogle Scholar
  18. 18.
    Fey, H., Margadant, A.: Zbl. Vet. Med. 9: 767 (1962).Google Scholar
  19. 19.
    Balfour, W. E., Comline, R. S.: J. Physiol. 160: 234 (1962).PubMedGoogle Scholar
  20. 20.
    Fey, H.: Die Bedeutung des Gammaglobulins für das neugeborene Kalb. Internat. Tierärztl. Arbeitsgemeinschaft Tierernährung, Symposium Salzburg, p. 17 (1967).Google Scholar
  21. 21.
    Kraehenbuhl, J. P., Campiche, M. A.: J. Cell Biol. 42: 345 (1969).PubMedCrossRefGoogle Scholar
  22. 22.
    Brambell, F. W.R.: The transmission of passive immunity from mother to young. In: Neuberger, A., Tatum, E. L.: Frontiers of Biology, vol. 18 ( North-Holland Publishing Co., Amsterdam-London 1970 ).Google Scholar
  23. 23.
    Kraehenbuhl, J. P., Gloor, E., Blanc, B.: Z. Zellforsch. 70: 209 (1966).PubMedCrossRefGoogle Scholar
  24. 24.
    Kraehenbuhl, J. P., Gloor, E., Blanc, B.: Z. Zellforsch. 76: 170 (1967).CrossRefGoogle Scholar
  25. 25.
    Clark, S. L.: J. biophys. biochem. Cytol. 5: 41 (1959).PubMedCrossRefGoogle Scholar
  26. 26.
    Moon, H.W.: Vet. Path. 9: 3 (1972).CrossRefGoogle Scholar
  27. 27.
    Halliday, R.: Proc. Roy. Soc. B 143: 408 (1955).CrossRefGoogle Scholar
  28. 28.
    Clarke, R. M„ Hardy, R. N.: J. Physiol. 209: 669 (1970).PubMedGoogle Scholar
  29. 29.
    Clarke, R. M., Hardy, R.N.: J. Anat. 108: 63 (1971).PubMedGoogle Scholar
  30. 30.
    Clarke, R. M., Hardy, R. N.: J. Physiol. 204: 127 (1969).PubMedGoogle Scholar
  31. 31.
    Moon, H. W., Kohler, E. M., Whipp, S. C.: Lab. Invest. 28: 23 (1973).PubMedGoogle Scholar
  32. 32.
    Koldovsky, O., Sunshine, P., Kretchmer, N.: Nature 212: 1389 (1966).PubMedCrossRefGoogle Scholar
  33. 33.
    Cornell, R., Walker, W.A., Isselbacher, K. J.: Lab. Invest. 25: 42 (1971).PubMedGoogle Scholar
  34. 34.
    Warshaw, A. L., Walker, W. A., Cornell, R., Isselbacher, K. J.: Lab. Invest. 25: 675 (1971).Google Scholar
  35. 35.
    Walker, W. A., Cornell, R., Davenport, L. M., Isselbacher, K. J.: J. Cell Biol. 54: 195 (1972).PubMedCrossRefGoogle Scholar
  36. 36.
    Walker, W. A., Isselbacher, K. J., Bloch, K. J.: Science 177: 608 (1972).Google Scholar
  37. 37.
    Walker, W. A., Isselbacher, K. J., Bloch, K. J.: J. Immunol. 111: 221 (1973).PubMedGoogle Scholar
  38. 38.
    Isomäki, A. M.: Acta path. microbiol. scand., Sect. A, Supplement 240 (1973).Google Scholar
  39. 39.
    Volkheimer, G., Hermann, H., Hermans, E., John, A., Al Abesie, F., Wachtel, S.: Zbl. Bakt. (Orig.) 192: 121 (1964).Google Scholar
  40. 40.
    Volkheimer, G., Schulz, F. H., John, H., Meier zu Eisen, J., Niederkorn, K.:Gynaecologica 168: 86 (1969).Google Scholar
  41. 41.
    Volkheimer, G., Schulz, F. H., Hofmann, I., Pieser, J., Rack, O., Reichelt, G., Rothenbaecher, W., Schmelich, G., Schurig, B., Teicher, G., Weiss, B.: Pharmacology 1: 8 (1968).Google Scholar
  42. 42.
    Volkheimer, G., Wendlandt, H., Wagemann, W., Reitzig, P., Schneider, D., Böhm, C., Böhm, M., Eras, B., Gröning, H., Hauptmann, G., Hiller, R., Lorenz, H., Mandelkow, A., Strauch, S.: Path. Microbiol. 31: 51 (1968).Google Scholar
  43. 43.
    Joel, D. D., Hess, M. W., Cottier, H.: J. exp. Med. 135: 907 (1972).PubMedCrossRefGoogle Scholar
  44. 44.
    Chanana, A. D., Schädeli, J., Hess, M. W., Cottier, H.: J. Immunol. 110: 283 (1973).Google Scholar
  45. 45.
    Joel, D. D., Sordat, B., Hess, M. W., Cottier, H.: Experientia 26: 694 (1970).Google Scholar
  46. 46.
    Hanaoka, M., Williams, M., Waksman, B. H.: Lab. Invest. 24: 31 (1971).Google Scholar
  47. 47.
    Friedenstein, A., Goncharenko, I.: Nature 206: 1113 (1965).PubMedCrossRefGoogle Scholar
  48. 48.
    Hess, M. W., Cottier, H., Sordat, B., Joel, D. D., Chanana, A. D.: The intestinal barrier to bacterial invasion. In: Braun, W., Ungar, J.: Non-Specific Factors Influencing Host Resistance. A Reexamination, p. 447 ( Karger, Basel 1973 ).Google Scholar
  49. 49.
    Shimizu, Y., Andrew, W.: J. Morphol. 123: 231 (1967).PubMedCrossRefGoogle Scholar
  50. 50.
    Waksman, B. H., Ozer, H., Blythman, H. E.: Lab. Invest. 28: 614 (1973).PubMedGoogle Scholar
  51. 51.
    Reimann, H. A.: JAMA 192: 100 (1965).CrossRefGoogle Scholar
  52. 52.
    Cooper, M. D., Lawton, A. R., Kincade, P. W.: Clin. exp. Immunol. 11: 143 (1972).Google Scholar
  53. 53.
    Alten van, P. J., Meuwissen, H. J.: Science 176: 45 (1972).PubMedCrossRefGoogle Scholar
  54. 54.
    Pollard, M., Sharon, N.: Infect. Immun. 2: 96 (1970).PubMedGoogle Scholar
  55. 55.
    Bienenstock, J., Dolezel, J.: J. Immunol. 106: 938 (1971).PubMedGoogle Scholar
  56. 56.
    Cooper, G. N., Turner, K.: J. Reticuloendoth. Soc. 6: 419 (1969).Google Scholar
  57. 57.
    Craig, S. W., Cebra, J. J.: J. exp. Med. 134: 188 (1971).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1975

Authors and Affiliations

  • H. Cottier
  • Th. Schaffner
  • A. D. Chanana
  • D. D. Joel
  • B. Sordat
  • B. J. Bryant
  • M. W. Hess

There are no affiliations available

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