Skip to main content

Radioautography as a Tool for the Study of Putative Neurotransmitters in the Nervous System

  • Chapter
Principles of Neurotransmission

Part of the book series: Journal of Neural Transmission ((NEURAL SUPPL,volume 12))

  • 36 Accesses

Abstract

The complete demonstration that the already known neurotransmitters in some synapses of the nervous system of invertebrates or in the peripheral nervous system of vertebrates play a similar role in the mammalian central synapses is still missing. Nevertheless the nature of the neurotransmitter present in some central pathways is well established: for instance GABA seems to be the neurotransmitter in the projections from the cerebellar cortex to the deep cerebellar nuclei and the vestibular nuclei (Obata and Takeda, 1969; Fonnum et al., 1970). However, there are only a few central specific synapses in which the exact nature of the neurotransmitter is completely demonstrated (i.e. the cholinergic nature [Eccles et al., 1954] of the synapses on Renshaw cells in the cat spinal cord).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Agid, Y., F. Javoy, J. Glowinski, D. Bouvet, and S. Sotelo; Injection of 6-hydroxydopamine into the substantia nigra of the rat. II. Diffusion and specificity. Brain Res. 58, 291–301 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Bisti, S., G. losif, G. F. Marchesi, and P. Strata: Pharmacological properties of inhibitions in the cerebellar cortex. Exp. Brain Res. 14, 24–37 (1971).

    Google Scholar 

  • Bloom, F. E.: Ultrastructural identification of catecholamine containing central synaptic terminals. J. Histochem. Cytochem. 21, 333–348 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Bloom, F. E., and L. L. Iversen: Localization of 3H-GABA in nerve terminals of rat cerebral cortex by electron microscopic autoradiography. Nature 229, 628–630 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Bowery, N. G., and D. A. Brown: y-aminobutyric acid uptake by sympathetic ganglia. Nature New Biol. 238, 89–91 (1972).

    Google Scholar 

  • Dahlstrom, A.: Observations on the accumulations of noradrenaline in the proximal and distal parts of peripheral adrenergic nerves after com-pression. J. Anat. (London) 99, 677–689 (1965).

    CAS  Google Scholar 

  • Dahlstrom, A., and K. Fuxe: Evidence for the existence of monoamine- containing neurons in the central nervous system. Acta physiol. Scand. suppl. 232, 62, 6–55 (1964).

    Google Scholar 

  • Descarries, L., and B. Droz: Intraneural distribution of exogenous nor-epinephrine in the central nervous system of the rat. J. Cell Biol. 44, 385–399 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Eccles, J. C., P. Fatt, and K. Koketsu: Cholinergic and inhibitory synapses in a pathway from motor-axon collaterals to motoneurones. J. Physiol. (London) 216, 524–562 (1954).

    Google Scholar 

  • Fonnum, F., J. Storm-Mathisen, and F. Walberg: Glutamate decarboxylase in inhibitory neurons. A study of the enzyme in Purkinje cell axons and boutons in the cat. Brain Res. 20, 259–275 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Geffen, L. B., L. Descarries, and B. Droz: Intraaxonal migration of nor-epinephrine injected into the coeliac ganglion of cats: radioautographic study of the proximal segment of constricted splenic nerves. Brain Res. 35, 315–318 (1971).

    Google Scholar 

  • Glowinksi, J., I. J. Kopin, and J. Axelrod: Metabolism of 3H-norepinephrine in the rat brain. J. Neurochem. 12, 25–30 (1965).

    Article  Google Scholar 

  • Grillo3 M. A.: C. Electron microscopy of sympathetic tissues. Pharmacol. Rev. 18, 387–399 (1966).

    PubMed  CAS  Google Scholar 

  • Gulley, R. L., and R. L. Wood: The fine structure of the neurons in the rat substantia nigra. Tissue and Cell 3, 675–690 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Hattori, T., P. L. McGeer, H. C. Fihiger, and E. G. McGeer: On the source of GABA-containing terminals in the substantia nigra. Electron microscopic autoradiographic and biochemical studies. Brain Res. 54, 103 to 114 (1973).

    Google Scholar 

  • H ok felt, T., and A. Ljungdahl: Cellular localization of labeled gamma- aminobutyric (3H-GABA) in rat cerebellar cortex; and autoradiographic study. Brain Res. 22, 391–396 (1970).

    Google Scholar 

  • H ok felt, T., and A. S. Ljungdahl: Histochemical determination of neuro-transmitter distribution. In: Neurotransmitters (Res. Publ. A.R.N.M.D.) Vol. 50, pp. 1–55 (1972 a).

    Google Scholar 

  • H ok felt, T., and A. Ljungdahl: Application of cytochemical techniques to the study of suspected transmitter substances in the nervous system. In: Studies of Neurotransmitters at the Synaptic Level (Costa, E., Iversen, L. L., and R. Paoletti), pp. 1–35 (Advances in Biochemical Psychopharmacology, Vol.6). New York: Raven Press. 1972 b.

    Google Scholar 

  • H ok felt, T., and A. Ljungdahl: Autoradiographic identification of cerebral and cerebellar cortical neurons accumulating labeled Gamma-amino- butyric acid (3H-GABA). Exp. Brain Res. 14, 354–362 (1972 c).

    Google Scholar 

  • Iversen, L. L.: The uptake and storage of noradrenaline in sympathetic nerves, p. 253. Cambridge University Press. 1967.

    Google Scholar 

  • Iversen, L. L., and F. E. Bloom: Studies of the uptake of 3H-GABA and 3H-glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography. Brain Res. 41, 131–143 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Iversen, L. L., J. S. Kelly, M. Minchin, F. Schon, and S. R. Snodgrass: Role of amino acids and peptides in synaptic transmission. Brain Res. 62, 567–576 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Iversen, L. L., and M. J. Neal: The uptake of 3H-GABA by slices of rat cerebral cortex. J. Neurochem. 15, 1141–1149 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Kawamura, H., and L. Provini: Depression of cerebellar Purkinje cells by microiontophoretic application of GABA and related amino acids. Brain Res. 24, 293–304 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Ljungdahl, A., A. Seiger, T. H ok felt, and L. Olson: 3H-GABA uptake in growing cerebellar tissue: autoradiography of intraocular transplants. Brain Res. 61, 379–384 (1973).

    Google Scholar 

  • Neal, M. J., and L. L. Iversen: Subcellular distribution of endogenous and 3H-y-aminobutyric acid in rat cerebral cortex. J. Neurochem. 16, 1245 to 1252 (1969).

    Google Scholar 

  • Neal, M. J., and L. L. Iversen: Autoradiographic localization of 3H-GABA in rat retina. Nature New Biology 235, 217–218 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Obata, K., and K. Takeda: Release of y-aminobutyric acid into the fourth ventricle induced by stimulation of the cat’s cerebellum. J. Neurochem. 16, 1043–1047 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Richards, J. G., and J. P. Trancer: The ultrastructural localization of amine storage sites in the central nervous system with the aid of a specific marker, 5-hydroxydopamine. Brain Res. 17, 463–469 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Roberts, E., and K. Kuriyama: Biochemical-physiological correlations in studies of the y-aminobutyric acid system. Brain Res. 8, 1–35 (1968).

    Article  PubMed  CAS  Google Scholar 

  • Schori, F., and L. L. Iversen: Selective accumulation of 3H-GABA by stellate cells in rat cerebellar cortex in vivo. Brain Res. 42, 503–507 (1972).

    Article  Google Scholar 

  • Sotelo, C.: The fine structural localization of norepinephrine-3H in the substantia nigra and area postrema of the rat. An autoradiographic study. J. Ultrastruct. Res. 36, 824–841 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Sotelo, C., A. Privât, and M. J. Drian: Localization of 3H-GABA in tissue culture of rat cerebellum using electron microscopy radioautography. Brain Res. 45, 302–308 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Sotelo, C., and J. Taxi: On the axonal migration of catecholamines in constricted sciatic nerve of the rat. A radioautographic study. 2. Zell- forsch. 138, 345–370 (1973).

    Google Scholar 

  • Taxi, J.: Contribution à l’étude des connexions des neurones moteurs du système nerveux autonome. In: Annales des Sciences Naturelles, 12e série, tome VII, Fasc. 3, pp. 413–674. Paris: Masson Ed. 1965.

    Google Scholar 

  • Taxi, J.: Morphological and cytochemical studies on the synapses in the autonomie nervous system. In: Progress in Brain Research, Vol. 31, pp. 17–20. Amsterdam: Elsevier. 1969.

    Google Scholar 

  • Taxi, J., and B. Droz: Radioautographic study of the accumulation of some biogenic amines in the autonomic nervous system. In: Symposium of the International Society for Cell Biology, Vol. 8, pp. 175–190. New York: Academic Press. 1969.

    Google Scholar 

  • Taxi, J., J. Gautron, and P. UHermite: Données ultrastructurales sur une éventuelle modulation adrénergique de l’activité du ganglion cervical supérieur du Rat. C.R. Acad. Se. Paris, t. 269, série D, 1281–1284 (1969).

    Google Scholar 

  • Taxi, J., and C. Sotelo: Cytological aspects of the axonal migration of catecholamines and of their storage material. Brain Res. 62, 431–437 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Wolfe, D. E., L. T. Potter, K. C. Richardson, and J. Axelrod: Localizing tritiated norepinephrine in sympathetic axons by electron microscopy autoradiography. Science 138, 440–442 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Young, J. A. C., D. A. Brown, J. S. Kelly, and F. Schon: Autoradiographic localization of sites of 3H-aminobutyric acid accumulation in peripheral autonomic ganglia. Brain Res. 63, 479–486 (1973).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1975 Springer-Verlag

About this chapter

Cite this chapter

Sotelo, C. (1975). Radioautography as a Tool for the Study of Putative Neurotransmitters in the Nervous System. In: Stockinger, L. (eds) Principles of Neurotransmission. Journal of Neural Transmission, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-8384-7_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-8384-7_4

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-8386-1

  • Online ISBN: 978-3-7091-8384-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics