The classic method of isolation of viruses from nature by the inoculation of laboratory animals or, in more recent years, of cell cultures, constitutes an important step in the selection of variants. Frequently, the newly-isolated strains will multiply with difficulty on first passage in their new host and will grow rapidly only after a certain number of passages. This is an example of population genetics, in which variants capable of multiplying in cells of a new host are being selected by continuous passage in this host. This sequence of events occurs with all viruses, including herpes simplex virus and pseudorabies virus.
KeywordsHerpes Simplex Chick Embryo Thymidine Kinase Cytopathic Effect Chorioallantoic Membrane
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