Harvesting efficacy and viability of retinal pigment epithelial cells in aspirates from posterior retinal areas — a study on human eyes
A reproducible cell amount in the inoculate and its high viability are critical factors in autologous RPE transplantation. We therefore applied a technique of gentle mobilization and aspiration of RPE-s from the nasal area of the retina to 14 cadaver human eyes. The outcome was systematically evaluated for RPE cell amounts and their viability. Briefly, after retinotomy the retina was locally detached nasally of the optic disc with a subretinal injection of Ringer’s solution. RPE cells were then gently mobilized over an area of 2–4 disc diameters, aspirated, stained with Trypan blue, and the dye exclusion immediately assessed. Total cell numbers ranged from 11.000 to 29.200 (19.976 ± 6.016), however, the harvesting efficacy (nr. of cells/disc area) was remarkably constant (6.165 ± 746). Cell viability was high (82,0 ± 6,9%). We conclude that reasonable cell numbers of high viability suitable for autologous RPE transplantation can be efficiently harvested even from small retinal areas.
KeywordsRetinal Pigment Epithelium Retinal Pigment Epithelial Cell Human Retinal Pigment Epithelial Cell Nasal Area Mobilization Area
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