Brain Edema pp 536-553 | Cite as

The Chemistry of Isolated Edema Fluid in Experimental Cerebral Injury

  • Raymond A. Clasen
  • Howard H. Sky-Peck
  • Sylvia Pandolfi
  • Iris Laing
  • George M. Hass


Focal areas of hemorrhagic necrosis were produced in the brains of anesthetized dogs by a freezing technique which has been described in previous publications (Clasen, et al., 1953). Briefly, this consisted of the application of a freezing instrument cooled by liquid nitrogen to the exposed intact skull. The instrument was left in place for five–ten minutes. This resulted in a circumscribed lesion involving both gray and white matter in the underlying brain. In the first hour after injury, cellular damage was restricted to the lesion. Four hours after injury, histologic evidence of edema was seen both in the lesion and in the adjacent white matter. Both areas showed positive staining by the periodic acid-Schiff (PAS) technique (Clasen, et al., 1957).


White Matter Edematous Tissue Edema Fluid Normal White Matter Cellulose Acetate Electrophoresis 
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Copyright information

© Springer-Verlag New York Inc. 1967

Authors and Affiliations

  • Raymond A. Clasen
  • Howard H. Sky-Peck
  • Sylvia Pandolfi
  • Iris Laing
  • George M. Hass

There are no affiliations available

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