Specificity of Electron Microscopic Localization of Phosphatase Activity to Cerebral Fine Structure
The localization of a reaction product formed as a result of enzyme activity to tissue fine structures has promised a new dimension to morphological techniques. In the brain this prophetic view-point was enhanced by the demonstration of nucleoside phosphatase activity within various specific components of the blood-brain barrier system of the rat (Torack & Barrnett). These and other membrane bound phosphatases are believed to be involved in membrane permeability (Saw, Schwartz), and ion transfer (Skou, Post, et al.), and their involvement in barrier control mechanisms appeared unlikely. However, a realization of the potential in this method was thwarted because the histochemical demonstration of these enzymes usually lacked the specificity of a biochemical technique. The precise nature of the enzyme or enzyme system responsible for the formation of the final product remained obscure.
KeywordsPhosphatase Activity Alkaline Phosphatase Activity Cobalt Sulfide Capture Reagent Electron Microscopic Localization
Unable to display preview. Download preview PDF.
- Gomori, G.: In: Microscopic Histochemistry: Principles and Practice. Chicago: Univ. Chicago Press (1952a).Google Scholar
- Gordon, J. S. and R. M. Torack: Inhibition of cerebral adenosine triphosphate (ATP’ase) activity by various aldehyde fixatives, J. Neurochem. (in press).Google Scholar
- Kaplan, N. O.: Symposium on multiple forms of enzymes and control mechanisms; I Multiple forms of enzymes, Bact. Rev., 27, 155–169 (1963).Google Scholar
- Saw, G. K.: Some concepts of the intracellular function of alkaline phosphatase based on investigation of the mechanism of their action, Enzymologia, 26, 169–175 (1963).Google Scholar
- Schwartz, J. H.: The phosphorylation of alkaline phosphatase, Proc. Acad. Sci. (United States), 49, 871–878 (1963).Google Scholar
- Taussky, H. H. and E. Shorr: A microcolorimetric method for the determination of inorganic phosphorus, J. Biol. Chem., 202, 675–505Google Scholar
- Tewari, H. B. and G. H. Bourne: Histochemical studies on the distribution of alkaline and acid phosphatase and 5′ nucleoside in the cerebellum of the rat, J. Anat. (London), 97, 65–72 (1963).Google Scholar
- Torack, R. M. and R. J. Barrnett: The fine structural localization of nucleoside phosphatase activity in the blood-brain barrier, J, Neuropath. Exp. Neurol., 23, 46–59 (1964).Google Scholar
- Torack, R. M. and R. J. Barrnett: The relationship between adenosinetriphosphatase activity and triethyltin toxicity in the production of cerebral edema of the rat. Amer. J. Pathr 46, 245–261 (1965b).Google Scholar
- Wachstein, M. and E. Meisel: Histochemistry of hepatic phosphatase at a physiologic pH with special reference to the demonstration of bile canaliculiy Amer. J. Path., 27, 13–27 (1957).Google Scholar