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Brain Edema pp 333-346 | Cite as

Brain Extracellular Space as Measured by Diffusion of Various Molecules into Brain

  • W. W. Oppelt
  • D. P. Rall

Abstract

There is currently controversy as to the extent of extracellular space (ECS) in the brain. Estimates of brain ECS range from 0–5% as reported from electron micrographs (Farquhar & Hartmann, 1957; Gershenfeld, et al., 1959; Horstmann & Meves, 1959; Maynard, et al., 1957; Schultz, et al., 1957; DeRobertis, 1962), to 50% when one equates in vitro sodium or chloride spaces to brain ECS (Manery & Bale, 1941; Elliott & Jasper, 1949).

Keywords

Caudate Nucleus Brain Edema Diffusion Profile Electron Microscope Picture Dead Brain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barlow, C. F., N. S. Domek, M. A. Goldberg and L. J. Roth: Extra-cellular brain space measured by S35 sulfate, Arch. Neurol. 5, 102–110 (1961).Google Scholar
  2. Bourke, R. S., E. S. Greenberg and D. B. Tower: Variation of cerebral cortex fluid spaces in vivo as a function of species brain size, Am. J. Physiol., 208, 689–692 (1965).Google Scholar
  3. Davson, H., C. F. Kleeman and E. Levin: The blood-brain barrier, in: Proceedings of the First International Pharmacology Meeting, Vol. 4: Drugs and Membranes (ed. by C. A. M. Hogben ), Pergamon Press (1963).Google Scholar
  4. DeRobertis, E.: Some old and new concepts of brain structure, World Neurol., 3, 98–111 (1962).Google Scholar
  5. Elliott, K. A. C. and H. Jasper: Measurement of experimentally induced brain swelling and shrinkage, Am. J. Physiol., 157, 122 (1949).PubMedGoogle Scholar
  6. Farquhar, M. G. and J. F. Hartmann: Neurological structure and relationships as revealed by electron microscopy, Neuropath. Exp. Neurol., 16, 18–39 (1957).Google Scholar
  7. Gershenfeld, H. M., F. Wald, J. A. Zadunaisky and E. D. P. DeRobertis: Function of atroglia in the water-ion metabolism of the central nervous system, Neurology, 9, 412–425 (1959).Google Scholar
  8. Horstmann, E. and H. Meves: Die Feinstruktur des molekularen Rindengraues und ihre physiologische Bedeuting, Z. Zellforsch. Mikroskop. Anat., 49, 569–604 (1959).CrossRefGoogle Scholar
  9. Karlsson, U. and R. L. Schultz: Fixation of the central nervous system for electron microscopy by aldehyde perfusion. I. Preservation with aldehyde perfusates versus direct perfusion with osmium tetroxide with special reference to membranes and the extracellular space, J. Ultrastruct. Res., 12, 160–186 (1965).Google Scholar
  10. Manery, J. F. and W. F. Bale: Penetration of radioactive sodium and phosphorus into the extra- and intracellular phases of tissues, Am. J. Physiol., 132, 215 (1941).Google Scholar
  11. Maynard, E. A., R. L. Schultz and D. C. Pease: Electron microscopy of the vascular bed of rat cerebral cortex, Am. J. Anat., 100, 409–433 (1957).PubMedCrossRefGoogle Scholar
  12. Oppelt, W. W., C. S. Patlak and D. P. Rail: Extracellular space in brain exposed to hypo- or hypertonic environments, Clin. Res., 12, 197 (1964).Google Scholar
  13. Pappius, H. M., I. Klatzo and K. A. C. Elliott: Further studies on swelling of brain slices, Canad. J. Biochem. Physiol., 40, 885–898 (1962).PubMedCrossRefGoogle Scholar
  14. Rail, D. P., W. W. Oppelt and C. S. Patlak: Extracellular space of brain as determined by diffusion of inulin from the ventricular system, Life Sci., 2, 43–48 (1962).CrossRefGoogle Scholar
  15. Schantz, E. J. and M. A. Laufer: Diffusion measurements in agar gell, Biochem., 1, 658–663 (1962).CrossRefGoogle Scholar
  16. Schultz, R. L., E. A. Maynard and D. C. Pease: Electron microscopy of neurons and neuroglia of cerebral cortex and corpus callosum, Am. J. Anat., 100, 369–408 (1957).PubMedCrossRefGoogle Scholar
  17. Schultz, R. L. and U. Karlsson: Fixation of the central nervous system for electron microscopy by aldehyde perfusion. II. Effect of osmolarity, pH of perfusate and fixative concentration, J. Ultrastruct. Res., 12, 187–206 (1965).PubMedCrossRefGoogle Scholar
  18. Streicher, E., D. P. Rail and J. R. Gaskins: Distribution of thiocyanate between plasma and cerebrospinal fluid, Am. J. Physiol., 206, 251–254 (1964).PubMedGoogle Scholar
  19. Van Harreveld, A. and J. Crowell: Extracellular space in central nervous tissue, Federation Proc., 23, 304 (1964).Google Scholar
  20. Van Harreveld and S. Ochs: Cerebral impedance changes after circulatory arrest, Am. J. Physiol., 187, 180–192 (1956).Google Scholar
  21. Van Harreveld, N. K. Hooper and J. T. Cusick: Brain electrolytes and cortical impedance, Ibid., 201, 139 - 143 (1961).Google Scholar
  22. Varon, S. and H. Mcllwain: Fluid contents and compartments in isolated cerebral tissue, J. Neurochem., 8, 262–275 (1961).PubMedCrossRefGoogle Scholar
  23. Woodbury, D. M.: in: Biology of Neuroglia (ed. by W. F. Windle). Springfield: Charles C. Thomas, pp. 120–128 (1958).Google Scholar
  24. Woodbury, D. M., P. S. Timiras, A. Koch and A. Ballard: Distribution of radio chloride, radiosulfate and inulin in brain of rats, Fed. Proc., 15, 501 (1956).Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1967

Authors and Affiliations

  • W. W. Oppelt
  • D. P. Rall

There are no affiliations available

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