Summary
Cultured bovine bronchial epithelial cells respond to thermal stress with induction of HSP 70 and this stress is associated with increased fibronectin release. We conclude that epithelial cells are capable of responding to stress and that altered release of mediators involved in repair may be part of the stress response. TGF-p may modulate HSP 70 expression, to which extent, however, needs further evaluation.
Epithelial cells play an important role in inflammatory processes such asthma and chronic bronchitis having the potential to communicate with a number of immune cells in order to help direct the inflammatory response.
In this context, epithelial cells influence processes in the airways including smooth muscle tone, inflammation, repair and fibrosis. Improved understanding of the role of epithelial cells in airway diseases will provide the means for more specific direction of new therapeutic strategies.
Zusammenfassung
Epithel unter Stress. Atemwegserkrankungen wie die chronische Bronchitis und das Asthma sind durch wiederholte Episoden der Atemwegsentzündung gekennzeichnet. Der Mechanismus bei der Reparation der entzündlichen Schäden ist heute noch weitgehend unklar, spielt aber eine Schlüsselrolle darin, ob bleibende Schäden wie die subepitheliale Fibrose sich entwickeln werden oder nicht. Moderne Konzepte deuten darauf hin, daß die Migration, Proliferation und Differenzierung der Atemwegsepithelien für die Reparation einer verletzten Oberfläche von essentieller Bedeutung sind.
Fibronektin, ein extrazelluläres Matrixglykoprotein, spielt eine bereits gut definierte Rolle bei zelluären Prozessen, welche für Reparationsmechanismen Bedeutung haben.
Hitzeschockproteine (HSPs, Streßprotein) werden in Säugetierzellen gebildet, wenn dieselben auf eine Reihe von Streßsituationen reagieren, wie etwa Hyperthermie, Hypoxie, Ischaemie und oxydative Schädigung. Bei Menschen zählt die Familie der 70 kD HSP zu den prominentesten Vertretern der Hitzeschockproteine.
HSPs sind jüngst im Atemwegsepithel gefunden worden, wo sie wohl eine weitere Schutzfunktion gegenüber Streß ausüben. In weiterer Folge konnte gezeigt werden, daß TGF-beta die Produktion von HSP vermehrt.
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References
34th Annual Thomas L (1992) Petty Aspen Lung Conference. Chest 101 (3) [Suppl]: 2–85
Lung Biology in Health and Disease. The Airway Epithelium. Lenfant C (ed) Marcel Dekker, New York
Laitinen LA, Heino M, Laitinen A, Kava T, Haahtela T (1985) Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis 131: 599–606
Dunnill MS (1960) The pathology of asthma, with special reference to changes in the bronchial mucosa. J Clin Path 13: 27–33
Lane BP, Gordon R (1974) Regeneration of rat tracheal epithelium after mechanical injury. Proc Soc Exp Biol Med 145: 1139–1144
McDowell EM, Ben T, Newkirk C, Chang S, DeLuca LM (1987) Differentiation of tracheal mucociliary epithelium in primary cell culture recapitulates normal fetal development and regeneration following injury in hampsters. Am J Pathol 129: 511–522
Rutten MJ, Ito S (1983) Morphology and electrophysiology of guinea pig gastric mucosal repair in vitro. Am J Physiol 244: 171–182
Moore R, Carlson S, Madara JL (1989) Rapid barrier restitution in an vitro model of intestinal epithelial injury. Lab Invest 60: 237–244
Jordana M, Clancy R, Dolovich J, Denburg J (1992) Effector role of the epithelial compartment in inflammation. Annals of the New York Academy of Sciences 664: 180–189
Churchill L, Chilton FH, Resau JH, Bascom R, Hubbard WC, Proud D (1989) Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells. Am Rev Respir Dis 140: 449–459
Mattoli S, Marini M, Fasoli A (1992) Expression of the potent inflammatory cytokines, GM-CSF, IL6, and IL8, in bronchial epithelial cells of asthmatic patients. Chest 101 (3) [Suppl]: 27–29
Koyama S, Rennard SI, Leikauf GD, Shoji S, VonEssen S, Claassen L, Robbins RA (1991) Endotoxin stimulates bronchial epithelial cells to release chemotactic factors for neutrophils. J Immunol 147: 4293–4301
Holtzman MJ, Hansbrough JR, Rosen GD, Turk J. Uptake release and novel species-dependent oxygenation of arachidonic acid in human and animal airway epithelial cells. Biochem Biophys Acta 963: 401–413
Noah TL, Paradiso AM, Madden MC, McKinnon KP, Devlin RB (1991) The response of a human bronchial epithelial cell line to histamine: intracellular calcium changes and extracellular release of inflammatory mediators. Am J Respir Cell Mol Biol 5: 484 - 492
Heeger P, Wolf G, Meyers C, Sun MJ, O*#x2019;Farrell SC, Krensky AM, Neilson EG (1992) Isolation and characterization of cDNA from renal tubular epithelium encoding murine RANTES. Kidney Intl 41: 220–225
Standiford TJ, Kunkel SL, Basha MA, Chensue SW, Lynch JP, III, Toews GB, Westwick J, Stricter RM (1990) Interleukin-8 gene expression by a pulmonary epithelial cell line: a model for cytokine networks in the lung. J Clin Invest 86: 1945–1953
Standiford TJ, Rolfe MW, Kunkel SL, Lynch III JJP, Burdick MD, Gilbert AR, Orringer MB, Whyte RI, Stricter RM (1993) Macrophage inflammatory protein-1 alpha expression in interstitial lung disease. J Immunol 151: 2852
Ohtoshi T, Vancheri C, Cox G, Gauldie J, Dolovich J, Denburg JA, Jordana M (1991) Monocyte-macrophage differentiation induced by human upper airway epithelial cells. Am J Respir Cell Mole Biol 4: 255–263
Ohtoshi T, Tsuda T, Vancheri C, Abrams JS, Gauldie J, Dolovich J, Denburg JA, Jordana M (1991) Human upper airway epithehal cell-derived granulocyte-macrophage colonystimulating factor induces histamine-containing cell differentiation of human progenitor cells. Int Arch Allergy Appl Immunol 95: 376–384
Marini M, Soloperto M, Mezzetti M, Fasoli A, Mattoli S (1991) Interleukin- 1 binds to specific receptors on human bronchial epithelial cells and upregulates granulocyte/macrophage colony-stimulating factor synthesis and release. Am J Respir Cell Mol Biol 4: 519–524
Rothlein R, Czajkowski M, O’Neill MM, Marlin SD, Mainolfi E, Merluzzi VJ (1988) Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. J Immunol 141: 1665–1669
Wegner CD, Gundel RH, Reilly P, Haynes N, Letts LG, Rothlein R (1990) Intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of asthma. Science 2: 456–459
Tosi MF, Stark JM, Hamedani A, Smith CW, Gruenerts DC, Huang YT (1992) Intercellular adhesion molecule-1 (ICAM-l)dependent and ICAM-1- independent adhesive interactions between polymorphonuclear leukocytes and human airway epithelial cells infected with parainfluenza virus type 2. J Immunol 149: 3345–3349
Nickoloff BJ, Turka LA (1993) Keratinocytes: key immunocytes of the integument. Am J Pathol 143 (2): 325–331
Keenan KP, Wilson TS, McDowell EM (1983) Regeneration of hamster tracheal epithelium after mechanical injury. IV. Histochemical, immuno- cytochemical and ultrastructural studies. Virchows Arch (Cell Pathol) 43: 213–240
McGowan S (1992) Extracellular matrix and the regulation of lung development and repair. FASEB J 6: 2895–2904
Grinnell F, Billingham RE, Burgess L (1981) Distribution of fibronectin during wound healing in vivo. J Invest Dermatol 76: 181–189
Romberger DJ, Beckmann JD, Claassen L, Ertl RE, Rennard SI (1992) Modulation of fibronectin production of bovine bronchial epithelial cells by transforming growth factor-ß. Am J Respir Cell Mol Biol 7: 149–155
Stoner GD, Katoh Y, Eoidart JM, Trump BE, Steinert PM, Harris CC (1981) Cultured human bronchial epithelial cells: blood group antigens, keratin, collagens, and fibronectin. In Vitro 17 (7): 577–587
Shoji S, Ertl RE, Linder J, Romberger DJ, Rennard SI (1990) Bronchial epithelial cells produce chemotactic activity for bronchial epithelial cells. Am Rev Respir Dis 141: 218–225
Morle A, Zhang Z, Ruoslahti E (1994) Superfibronectin is a functionally distinct form of fibronectin. Nature 367: 193–196
Ruoslahti E (1988) Fibronectin and its receptors. Annu Rev Biochem 57: 375–413
Wang A, Cohen DS, Palmer E, Sheppard D (1991) Polarized regulation of fibronectin secretion and alternative splicing by transforming growth factor ß. J Biol Chem 266: 15598–15601
Sacco O, Romberger D, Rizzino A, Beckmann J, Rennard SI, Spurzem J (1992) Spontaneous production of TGF-ß2 by primary cultures of bronchial epithelial cells: effects on cell behavior in vitro. J Clin Invest 90: 1379–1385
Border W, Ruoslahti E (1992) Transforming growth factor-ß in disease: the dark side of tissue repair. J Clin Invest 90: 1–7
Spurzem JR, Sacco O, Rickard KA, Rennard SI (1993) Transforming growth factor-beta increases adhesion but not migration of bovine bronchial epithelial cells to matrix proteins. J Lab Clin Med 122: 92–102
Bonay M, Soler P, Riquet M, Battesti JP, Hance AJ, Tozi A (1994) Expression of heat shock proteins in human lung and lung cancer. Am J Respir Cell Mol Biol 10: 453–461
Polla SP (1988) A role for heat shock proteins in inflammation. Immunology Today 9: 134–137
Morimoto RI (1993) Cells in stress: transcriptional activation of heat shock genes. Science 259: 1409–1410
Takenaka J, Hightower LE (1992) Transforming growth factor-ßl rapidly induces Hsp70 and Hsp90 molecular chaperous in cultured chicken embryo cells. J Cell Biol 152: 568–577
Prouse B, Pladsen P, Pohl W, Rennard SI, Romberger DJ (1994) Thermal stress induces bronchial epithelial cell heat shock protein expression and fibronectin release. Resp Crit Care Med 149: 996
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Pohl, W.R., Romberger, D.J. (1995). Epithelium under stress. In: Kummer, F. (eds) Asthma. Springer, Vienna. https://doi.org/10.1007/978-3-7091-7539-2_4
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DOI: https://doi.org/10.1007/978-3-7091-7539-2_4
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