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Characterization of a new Marburg virus isolated from a 1987 fatal case in Kenya

  • E. D. Johnson
  • B. K. Johnson
  • D. Silverstein
  • P. Tukei
  • T. W. Geisbert
  • A. N. Sanchez
  • P. B. Jahrling
Part of the Archives of Virology Supplement II book series (ARCHIVES SUPPL, volume 11)

Summary

In 1987, an isolated case of fatal Marburg disease was recognized during routine clinical haemorrhagic fever virus surveillance conducted in Kenya. This report describes the isolation and partial characterization of the new Marburg virus (strain Ravn) isolated from this case. The Ravn isolate was indistinguishable from reference Marburg virus strains by cross-neutralization testing. Virus particles and aggregates of Marburg nucleocapsid matrix in Ravn-infected vero cells, were visualized by immunoelectron microscopic techniques, and also in tissues obtained from the patient and from inoculated monkeys. The cell culture isolate produced a haemorrhagic disease typical of Marburg virus infection when inoculated into rhesus monkeys. Disease was characterized by the sudden appearance of fever and anorexia within 4 to 7 days, and death by day 11. Comparison of nucleotide sequences for portions of the glycoprotein genes of Marburg-Ravn were compared with Marburg reference strains Musoki (MUS) and Popp (POP). Nucleotide identity in this alignment between RAV and MUS is 72.3%, RAV and POP is 71%, and MUS and POP is 91.7%. Amino acid identity between RAV and MUS is 72%, RAV and POP is 67%, and MUS and POP is 93%. These data suggest that Ravn is another subtype of Marburg virus, analogous to the emerging picture of a spectrum of Ebola geographic isolates and subtypes.

Keywords

Disseminate Intravascular Coagulation Plaque Form Unit Rift Valley Fever Rift Valley Fever Virus Pyrimethamine Sulfadoxine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Arata AA, Johnson BK (1978) Approaches towards studies of potential reservoirs of viral haemorrhagic fever in Southern Sudan (1977). In: Pattyn SR (ed) Ebola virus hemorrhagic fever. Elsevier/North-Holland Biomedical Press, Amsterdam, pp 191–200Google Scholar
  2. 2.
    Centers for Disease Control and Prevention (1995) Update: outbreak of Ebola viral haemorrhagic fever-Zaire, 1995. MMWR 44: 381–382Google Scholar
  3. 3.
    Centers for Disease Control (1995) Management of patients with suspected viral haemorrhagic fever. MMWR 44: 475–479Google Scholar
  4. 4.
    Ghomczynski P, Sacchi N (1987) Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159CrossRefGoogle Scholar
  5. 5.
    Clauss A (1957) Rapid physiological coagulation method in determination of fibrinogen. Acta Haematol 17: 237–246PubMedCrossRefGoogle Scholar
  6. 6.
    Conrad JL, Isaacson M, Smith EB, Wulff H, Grees M, Gildenhags P, Johnson J (1978) Epidemiologic investigation of Marburg virus disease South Africa, 1975. Am J Trop Med Hyg 27: 1210–1215PubMedGoogle Scholar
  7. 7.
    Feldmann H, Klenk HD, Sanchez A (1993) Molecular biology and evolution of filoviruses. In: Kaaden OR, Eichhorn W, Czerny CP (eds) Unconventional agents and unclassified viruses. Recent advances in biology and epidemiology. Springer, Wien New York, pp 81–100 (Arch Virol [Suppl] 7)Google Scholar
  8. 8.
    Geisbert TW, Jahrling PB (1995) Differentiation of filoviruses by electron microscopy. Virus Res 39: 129–150PubMedCrossRefGoogle Scholar
  9. 9.
    Gear IS, Cassel GA, Geor AJ, Troppler B, Clausen L, Meyers AM, Kew MC, Bothwell TH, Sher R, Miller GB, Schneider J, Koomhof HJ, Gompets ED, Isaacson M, Geor JHS (1975) Outbreak of Marburg virus disease in Johannesburg. Br Med J 4: 489–493PubMedCrossRefGoogle Scholar
  10. 10.
    Hawiger J (1980) Measurement of fibrinogen and fibrin degradation products in serum by staphylococcal clumping test. J Lab Clin Med 75: 93–108Google Scholar
  11. 11.
    Jahrling PB, Geisbert TW, Jaax NK, Hanes MA, Ksiazek TG, Peters CJ (1996) Experimental infection of cynomolgus macaques with Ebola-Reston filoviruses from the 1989–1990 U.S. epizootic. In: Schwarz TF, Siegl G (eds) Imported virus infections. Springer, Wien New York, pp 115–134 (Arch Virol [Suppl] 11)Google Scholar
  12. 12.
    Jahrling PB (1995) Filoviruses and arenaviruses. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds) Manual of clinical microbiology, 6th ed. ASM Press, Washington, pp 1068–1081Google Scholar
  13. 13.
    Jahrling PB, Geisbert TW, Dalgard DW, Johnson ED, Ksiazek TG, Hall WC, Peters CJ (1990) Preliminary report: isolation of Ebola virus from monkeys imported to the USA. Lancet 335: 502–505PubMedCrossRefGoogle Scholar
  14. 14.
    Johnson KM, Elliott LH, Heymann DL (1981) Preparation of polyvalent viral immuno-fluorescent intracellular antigens and use in human serosurveys. J Clin Microbiol 14: 527–529PubMedGoogle Scholar
  15. 15.
    Martini GA, Knauff HG, Schmidt HA, Mayer G, Baetzer G (1968) A hitherto unknown infectious disease contracted from monkeys. Marburg virus disease. Ger Med Mon 13: 457–470PubMedGoogle Scholar
  16. 16.
    Le Guenno B, Formentry P, Wyers M, Gounon P, Walker F, Boesch C (1995) Isolation and partial characterization of a new strain of Ebola virus. Lancet 345: 1271–1274PubMedCrossRefGoogle Scholar
  17. 17.
    Peters CJ, Sanchez A, Feldmann A, Rollin PE, Nichol S, Ksiazek TG (1994) Filoviruses as emerging pathogens. Semin Virol 5: 147–154CrossRefGoogle Scholar
  18. 18.
    Siegert R (1970) The Marburg virus (vervet monkey agent). Mod Trends Med Virol 32: 204–240Google Scholar
  19. 19.
    Simpson DIH, Zlotnbik I, Rutter DA (1968) Vervet monkey disease. Experimental infection of guinea pigs and monkeys with the causative agent. Br J Exp Pathol 69: 458–464Google Scholar
  20. 20.
    Smith DH, Johnson BK, Isaacson M, Swanepoel R, Johnson KM, Kiley MP, Bagshawe A, Siangok T, Keruga W (1982) Marburg virus disease in Kenya. Lancet i: 816–820CrossRefGoogle Scholar
  21. 21.
    Triplett DA, Smith C (1982) Routine testing in the coagulation laboratory. In: Triplett DA (ed) Laboratory evaluation of coagulation. American Society of Clinical Pathologists Press, Chicago, pp 27–52Google Scholar

Copyright information

© Springer-Verlag/Wien 1996

Authors and Affiliations

  • E. D. Johnson
    • 1
  • B. K. Johnson
    • 2
  • D. Silverstein
    • 3
  • P. Tukei
    • 2
  • T. W. Geisbert
    • 1
  • A. N. Sanchez
    • 4
  • P. B. Jahrling
    • 1
  1. 1.Fort DetrickUnited States Army Medical Research Institute of Infectious DiseasesFrederickUSA
  2. 2.Virus Research CenterKenya Medical Research InstituteKenya
  3. 3.The Nairobi HospitalNairobiKenya
  4. 4.Special Pathogens BranchCenters for Disease ControlAtlantaUSA

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