Auxotroph Isolation In Vitro

  • Anne D. Blonstein
Part of the Plant Gene Research book series (GENE)


Following the first report of a nutritional mutant in higher plants (Lang- ridge & Brock, 1961), just ten years elapsed before auxotrophic mutants were declared to have been produced from plant somatic cell culture (Carlson, 1970), and the future looked bright for the application of in vitro techniques in the induction and isolation of “novel” plant variation. Nevertheless, in 1980 Robert Shields, reviewing a book on plant cell and tissue culture, commented that there is a “paucity of useful selectable mutations in defined genes in plant cells” even though with the availability of haploid material “recessive mutations should be obtainable at reasonable frequency”. He went on to say, “one can only assume that not enough people have tried hard enough.”


Plant Regeneration Protoplast Culture Auxotrophic Mutant Fusion Hybrid Mutant Isolation 
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© Springer-Verlag/Wien 1986

Authors and Affiliations

  • Anne D. Blonstein
    • 1
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland

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