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Auxotroph Isolation In Vitro

  • Chapter
A Genetic Approach to Plant Biochemistry

Part of the book series: Plant Gene Research ((GENE))

Abstract

Following the first report of a nutritional mutant in higher plants (Lang- ridge & Brock, 1961), just ten years elapsed before auxotrophic mutants were declared to have been produced from plant somatic cell culture (Carlson, 1970), and the future looked bright for the application of in vitro techniques in the induction and isolation of “novel” plant variation. Nevertheless, in 1980 Robert Shields, reviewing a book on plant cell and tissue culture, commented that there is a “paucity of useful selectable mutations in defined genes in plant cells” even though with the availability of haploid material “recessive mutations should be obtainable at reasonable frequency”. He went on to say, “one can only assume that not enough people have tried hard enough.”

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  1. Friedrich Miescher-Institut, P. O. Box 2543, CH-4002, Basel, Switzerland

    Anne D. Blonstein

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    Anne D. Blonstein

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    Patrick J. King

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Blonstein, A.D. (1986). Auxotroph Isolation In Vitro . In: Blonstein, A.D., King, P.J. (eds) A Genetic Approach to Plant Biochemistry. Plant Gene Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6989-6_10

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  • DOI: https://doi.org/10.1007/978-3-7091-6989-6_10

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