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Direct Gene Transfer to Plants

  • I. Potrykus
  • J. Paszkowski
  • R. D. Shillito
  • M. W. Saul
Part of the Plant Gene Research book series (GENE)

Abstract

Recent progress in cell biology and molecular biology has made available a variety of techniques enabling gene transfer across the borders set by the biology of pollination and fertilisation. These novel techniques include “somatic hybridisation” leading to the combination of entire genomes (Gleba and Sytnik, 1984), “subprotoplast fusion” yielding the transfer of a partial genome (Maliga et al., 1982), X-irradiation prior to protoplast fusion to achieve organelle transfer (Aviv et al., 1980), transfer of isolated nuclei (King and Saxena, Saxena and King, 1985) and metaphase chromosomes (DeLaat and Blaas, 1985). Besides these methods which often cause unpredictable and severe alterations of the host genome, there are others which introduce not more than small stretches of foreign DNA, often not more than just a single foreign gene. For years this has been possible only via the bacterial vector Agrobacterium tumefaciens. At present more methods of gene transfer to plants are available. Gene transfer via A. tumefaciens has already been discussed on many occasions (for a recent review see Gheysen et al., 1985 and chapter 7 in this volume). This volume contains also articles which deal with the use of viral vectors (see chapters 1 and 2) and the combination of A. tumefaciens and viral vectors — Agroinfection (chapter 4) therefore we shall concentrate only on direct, vectorless gene transfer to plants.

Keywords

Gene Transfer Foreign Gene Transformation Frequency Protoplast Fusion Selectable Marker Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1987

Authors and Affiliations

  • I. Potrykus
    • 1
  • J. Paszkowski
    • 1
  • R. D. Shillito
    • 1
  • M. W. Saul
    • 1
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland

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