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Molecular Analysis of Root Induction by Agrobacterium rhizogenes

  • Frank F. White
  • Vilas P. Sinkar
Part of the Plant Gene Research book series (GENE)

Abstract

Agrobacterium rhizogenes incites hairy root disease of plants. The disease is closely related to crown gall disease due to strains of Agrobacterium tumefaciens. The ability of both bacteria to cause plant tumors is encoded by the large virulence plasmids in the respective strains. The virulence plasmid of A. rhizogenes has become known as the root-inducing (Ri) plasmid, to distinguish the plasmid from the tumor-inducing (Ti) plasmid of A. tumefaciens. The underlying mechanisms for hairy root and crown gall disease are very similar. Therefore, this review will not discuss the extensive literature pertaining to the Ti plasmid except where necessary for comparison to the Ri plasmid. However, an understanding of the Ri plasmid as permitted by present knowledge is incomplete without consideration of the Ti plasmid, and the reader is referred to other chapters in this book and recent reviews (Zambryski et al., 1983; Nester et al., 1984).

Keywords

Hairy Root Root Induction Crown Gall Agrobacterium Rhizogenes Crown Gall Tumor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ackermann, C., 1977: Pflanzen aus Agrobacterium rhizogenes Tumoren an Nicotiana tabacum. Plant Sei. Lett. 8, 23–30.CrossRefGoogle Scholar
  2. Akiyoshi, D. E., Klee, H. J., Amasino, R, Nester, E. W., Gordon, M. P., 1984: T-DNA of Agrobacterium tumefaciens codes an enzyme of cytokinin biosynthesis. Proc. Natl. Acad. Sei., U.S.A. 81, 5994–5998.CrossRefGoogle Scholar
  3. Albinger, G., Beiderbeck, R, 1977: Übertragung der Fähigkeit zur Wurzelinduktion von Agrobacterium rhizogenes auf A. tumefaciens. Phytopathol. Z. 90, 306–310.CrossRefGoogle Scholar
  4. Amasino, R M., Powell, A. T., Gordon, M. P., 1984: Changes in T-DNA methyl- ation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line. Mol. & Gen. Genet. 197, 437–446.CrossRefGoogle Scholar
  5. An, G., Watson, B. D., Stachel, S., Gordon, M. P., Nester, E. W., 1985: New cloning vehicles for transformation of higher plants. EMBO J. 4, 277–284.PubMedGoogle Scholar
  6. Anderson, A. R, Moore, L. W., 1979: Host specificity in the genus Agrobacterium. Phytopathology 69, 320–323.CrossRefGoogle Scholar
  7. Ark, P. A., Thompson, J. P., 1961: Detection of hairy root pathogen, Agrobacterium rhizogenes, by use of fleshy roots. Phytopathology 51, 69–71.Google Scholar
  8. Barker, R F., Idler, K. B., Thompson, D. V., Kemp, J. D., 1983: Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti Plasmid pTil5955. Plant Mol. Bio. 2, 335–350.Google Scholar
  9. Barton, K. A., Binns, A. N., Matzke, A. J., Chilton, M.-D., 1983: Regeneration of intact tobacco plants containing full length copies of genetically engineered T-DNA and transmission of T-DNA to R1 progeny. Cell 32, 1033–1043.PubMedCrossRefGoogle Scholar
  10. Benvenuto, E., Ancora, G., Spano, L., Costantino, P., 1983: Morphogenesis and isoperoxidase characterization in tobacco “Hairy Root” regenerants. F. Pflan- zenphysiol. 110, 239–245.Google Scholar
  11. Black, R. C., Kuleck, G. A., Binns, A. N., 1986: The initiation of auxin autonomy in tissue from tobacco plants carrying the auxin biosynthesizing genes from the T-DNA of Agrobacterium tumefaciens. Plant Physiol. 80, 145–151.PubMedCrossRefGoogle Scholar
  12. Brown, N. A, 1929: The tendency of the crown-gall organism to produce roots in conjunction with tumors. J. Agr. Res. 39, 747–766.Google Scholar
  13. Byrne, M. C., Koplow, J., David, C., Tempe, J., Chilton, M.-D., 1983: Structure of T-DNA in roots transformed by Agrobacterium rhizogenes. J. Mol. Appl. Genet. 2, 201–209.PubMedGoogle Scholar
  14. Cardarelli, M., Spano, L., De Paolis, A., Mauro., M. L., Yitali, G., Costantino, P., 1985: Identification of the genetic locus responsible for non-polar root induction by Agrobacterium rhizogenes 1855. Plant Mol. Biol. 5, 385–391.Google Scholar
  15. Chilton, M.-D., Tepfer, D. A., Petit, A., David, C., Casse-Delbart, F., Tempe, J., 1982: Agrobacterium rhizogenes inserts a T-DNA into the genomes of the host plant root cells. Nature 295, 432 — 434.Google Scholar
  16. Comai, L., Facciotti, D., Hiatt, W. R., Thompson, G., Rose, R. E., Stalker, D. M., 1985: Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317, 741 — 744.CrossRefGoogle Scholar
  17. Costantino, P., Mauro, M. L., Micheli, G., Risuleo, G., Hooykaas, P. J. J., Schilperoort, R., 1981: Fingerprinting and sequence homology of plasmids from different virulent strains by Agrobacterium rhizogenes. Plasmid 5, 170–182.PubMedCrossRefGoogle Scholar
  18. Costantino, P., Spano, L., Pomponi, M., Benvenuto, E., Ancora, G., 1984: The T-DNA of Agrobacterium rhizogenes is transmitted through meiosis to the progeny of hairy root plants. J. Mol. Appl. Genet. 2, 465–470.Google Scholar
  19. Currier, T. C., Nester, E. W., 1976: Evidence for diverse types of large plasmids in tumor-inducing strains of Agrobacterium. J. Bacteriol. 126, 157–165.PubMedGoogle Scholar
  20. David, C., Chilton, M.-D., Tempe, J., 1984: Conservation of T-DNA in plants regenerated from hairy root cultures. Biotechnology 2, 73–76.CrossRefGoogle Scholar
  21. De Cleene, M. D., De Ley, J., 1981: The host range of infectious hairy root. Bot. Rev. 47, 147–194.CrossRefGoogle Scholar
  22. De Greve, H., Leemans, J., Hernalsteens, J. P., Thia-Toong, L., De Beuekeleer, M., Willmitzer, L., Otten, L., Van Montagu, M., Schell, J., 1982: Regeneration of normal and fertile plants that express octopine synthase, from tobacco crown galls after deletion of tumor-controlling functions. Nature 300, 752–755.CrossRefGoogle Scholar
  23. De Paolis, A., Mauro, M. L., Pomponi, M., Cardarelli, M., Spano, L., Costantino, P., 1985: Localization of Agropine-synthesizing functions in the Tr region of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid 13, 1–7.PubMedCrossRefGoogle Scholar
  24. Depicker, A., Herman, L., Jacobs, A., Schell, J., Van Montagu, M., 1985: Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium /plant cell interaction. Mol. & Gen. Genet. 201, 477 — 484.CrossRefGoogle Scholar
  25. Durand-Tardif, M., Broglie, R., Slightom, J., Tepfer, D., 1985: Structure and expression of Ri T-DNA from Agrobacterium rhizogenes in Nicotiana tabacum an phenotypic specificity. J. Mol. Biol. 186, 557–564.PubMedCrossRefGoogle Scholar
  26. Ellis, J. G., Murphy, P. J., 1981: Four new opines from crown gall tumors — their detection and properties. Mol. & Gen. Genet. 181, 36–43.Google Scholar
  27. Estramareix, C., Ratet, P., Boulanger, F., Richaud, F., 1986: Multiple mutations ir the transferred regions of the Agrobacterium rhizogenes root-inducing plasmids. Plasmid 15, 245–247.PubMedCrossRefGoogle Scholar
  28. Furner, I. J., Huffman, G. A., Amasino, R. M., Garfinkel, D. J., Gordon, M. P., Nester, E. W., 1986: An Agrobacterium transformation in the evolution of the genus Nicotiana. Nature 329, 422–427.CrossRefGoogle Scholar
  29. Garfinkel, D. J., Nester, E. W., 1980: Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J. Bacteriol. 144, 732–743.Google Scholar
  30. Garfinkel, D. J., Simpson, R. B., Ream, L. W., White, F. F., Gordon, M. P., Nester, E. W., 1981: Genetic analysis of crown gall: fine structure map of the T-DNA by site-directed mutagenesis. Cell 27, 143–153.PubMedCrossRefGoogle Scholar
  31. Hedgecock, G. G., 1905: The crown-gall hairy root disease of the apple tree. U.S. Dept. Agr. Bur. Plant Indus. Bull. 131, 21–23.Google Scholar
  32. Hoekema, A., Hooykaas, P. J., Schilperoort, R., A., 1984: Transfer of the octopine T-DNA segment to plant cells mediated by different types of Agrobacterium tumor- or root-inducing plasmids. Generality of virulence systems. J. Bacteriol. 158, 383–385.Google Scholar
  33. Holmes, B., Roberts, P., 1981: The classification, identification and nomenclature of Agrobacteria. J. Appl. Bacteriol. 50, 443–467.Google Scholar
  34. Holsters, M., Silva, V., Van Vliet, F., Genetello, C., DeBlock, M., Dhaese, P., Depicker, A., Inze, D., Engler, G., Villarroel, R., Van Montagu, M., Schell, J., 1980: The functional organization of the nopaline A. Tumefaciens plasmid pTi C58. Plasmid 3, 212–230.PubMedCrossRefGoogle Scholar
  35. Hood, E. E., Jen, G., Kayes, L., Kramer, J., Fraley, R. T., Chilton, M.-D., 1984: Restriction endonuclease map of pTIBo542, a potential Ti plasmid vector for genetic engineering of plants. Biotechnology 2, 702–709.CrossRefGoogle Scholar
  36. Hooykaas, P. J. J., Hofker, M., den Dulk-Ras, H., Schilperoort, R. A., 1984: A comparison of virulence determinants in an octopine Ti plasmid, a nopaline Ti plasmid, and an Ri plasmid by complementation analysis of Agrobacterium tumefaciens mutants. Plasmid 11, 195–205.PubMedCrossRefGoogle Scholar
  37. Hooykaas, P. J. J., Klapwijk, P. M., Nuti, M. P., Schilperoort, R. A., Rorsch, A., 1977: Transfer of the Agrobacterium tumefaciens Ti plasmid to avirulent Agrobacterium and to Rhizobium ex planta. J. Gen. Microbiol. 98, 477–484.Google Scholar
  38. Huffman, G. A., White, F. F., Gordon, M. P., Nester, E. W., 1984: Hairy-root- inducing plasmid: Pysical map and homology to tumor-inducing plasmids. J. Bacteriol. 157, 269–276.PubMedGoogle Scholar
  39. Inze, D., Follin, A., Van Lijsebettens, M., Simoens, C., Genetello, C., Van Montagu, M., Schell, J., 1984: Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens; further evidence that two genes are involved in indole- 3-acetic acid synthesis. Mol. & Gen. Genet. 194, 265–274.Google Scholar
  40. Jensen, J. S., Marcker, K. A., Otten, L., Schell, J., 1986: Nodule-specific expression of a chimaeric soybean leghaemoglobin gene in transgenic Lotus corniculatus. Nature 321, 669–675.CrossRefGoogle Scholar
  41. Joos, H., Inze, D., Caplan, A., Sormann, M., Van Montagu, M., Schell, J., 1983: Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell 32, 1057–1067.PubMedCrossRefGoogle Scholar
  42. Jouanin, L., 1984: Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 12, 91–102.PubMedCrossRefGoogle Scholar
  43. Jouanin, L., Vilaine, F., d’Eufert, C., Casse-Delbart, F., 1985: Localization and restriction maps of the replication origin regions of the plasmids of Agrobacterium rhizogenes strain A4. Mol. & Gen. Genet. 201, 370–374.CrossRefGoogle Scholar
  44. Keane, P. J., Kerr, A., New, P. B., 1970: Crown gall of stone fruit. Aust. J. Biol. Sei. 23, 585–595.Google Scholar
  45. Kerr, A., Roberts, W. P, 1976: Agrobacterium: Correlation between and transfer of pathogenicity, octopine and nopaline catabolism and bacteriocin 84 sensitivity. Physiol. Plant Pathol. 4, 37–44.Google Scholar
  46. Kersters, K., De Ley, J., 1973: Numerical taxonomic analysis of Agrobacterium. J. Gen. Microbiol. 78, 227–239.Google Scholar
  47. Klee, H, J, White, F. F., Iyer, V. N, Gordon, M. P, Nester, E. W, 1983: Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti- plasmid. J. Bacterid. 153, 878–883.Google Scholar
  48. Klee, H. J., Yanofsky, M. F., Nester, E. W., 1985: Vectors for transformation of higher plants. Biotechnology 3, 637–642.CrossRefGoogle Scholar
  49. Koplow, J., Byrne, M., Jen, Ge., Tempe, J., Chilton, M., 1984: Physical map of the Agrobacterium rhizogenes strain 8196 virulence plasmid. Plasmid 11, 17–27.PubMedCrossRefGoogle Scholar
  50. Lahners, K. Byrne, M. C., Chilton, M., 1984: T-DNA fragments of hairy root plasmid pRi8196 are distantly related to octopine and nopaline Ti plasmid T-DNA. Plasmid 11, 130–140.Google Scholar
  51. Moore, L., Warren, G., Strobel, G., 1979: Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizogenes. Plasmid 2, 617–626.PubMedCrossRefGoogle Scholar
  52. Munnecke, D. E., Chandler, P. A., Starr, M. P., 1963: Hairy root (Agrobacterium rhizogenes) of field roses. Phytopathology 53, 788–799.Google Scholar
  53. Nester, E. W., Gordon, M. P., Amasino, R. M., Yanofsky, M. F., 1984: Crown gall: a molecular and physiological analysis. Annu. Rev. Plant Physiol. 35, 387–413.CrossRefGoogle Scholar
  54. Ooms, G., Atkinson, J., Bossen, M. E., Leigh, R. A., 1986 a: TL-DNA from Agrobacterium rhizogenes plasmid pRi 1855 reduces the osmotic pressure in transformed plants grown in vitro. Planta 168, 106–112.Google Scholar
  55. Ooms, G., Karp, A., Burrell, M. M., Twell, D., Roberts, J., 1985: Genetic modification of potato development using Ri T-DNA. Theor. Appl. Genet. 70, 440–446.Google Scholar
  56. Ooms, G., Twell, D., Bossen, M. E., Höge, J. H. C., Burrell, M. M., 1986 b: Developmental regulation of Ri TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree). Plant Mol. Biol. 6, 321–330.Google Scholar
  57. Petit, A., David, C., Dahl, G., Ellis, J., Guyon, P., Casse-Delbart, F., Tempe, J., 1983: Further extension of the opine concept: Plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol. & Gen. Genet. 190, 204–214.CrossRefGoogle Scholar
  58. Pomponi, M., Spano, L., Sabbadini, M. G., Costantino, P., 1983: Restriction endo- nuclease mapping of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid 10, 119–129.PubMedCrossRefGoogle Scholar
  59. Riker, A. J., Banfield, W. M., Wright, W. H., Keitt, G. W., Sagen, H. E., 1930: Studies on infections hairy-root of nursery apple trees. J. Agric. Res. 41, 507–540.Google Scholar
  60. Risuleo, G., Battistoni, P., Costantino, P., 1982: Regions of homology between tumorigenic plasmids from Agrobacterium rhizogenes and Agrobacterium tumefaciens. Plasmid 7, 45–51.PubMedCrossRefGoogle Scholar
  61. Ryder, M. H., Tate, M. E., Kerr, A., 1985: Virulence properties of strains of Agrobacterium on the apical and basal surfaces of carrot root discs. Plant Physiol. 77, 215–221.PubMedCrossRefGoogle Scholar
  62. Schell, J., Van Montagu, M., Depicker, A., DeWaele, D., Engler, G., Genetello, C., Hernalsteens, J. O., Holsters, M., Messens, E., Silva, B., Van den Elsacken, S., Van Larabeke, N., Zaenen, I., 1976: Agrobacterium tumefaciens: what segment of the plasmid is responsible for the induction of crown gall tissue? In L. Bogorad and J. H. Weil (ed.) Nucleic Acids and Protein Synthesis in Plants. New York: Plenum Press, pp. 329–342.Google Scholar
  63. Schroder, G., Waffenschmidt, S., Weiler, E. W., Schroder, J., 1984: The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur. J. Biochem. 138, 387–391.PubMedCrossRefGoogle Scholar
  64. Siegler, E. A., 1928: Studies on the etiology of apple crown gall. J. Agr. Res. 37, 301–313.Google Scholar
  65. Skoog, F., Miller, C. O., 1957: Chemical regulation of growth and organ formation in plant tissues cultured in vitro. — The Biological Action of Growth Substances. Symposium XI, Soc. Exp. Biol. Cambridge Univ. Press, Cambridge, p. 118.Google Scholar
  66. Slightom, J. L., Durand-Tardif, M., Jouanin, L„ Tepfer, D., 1986: Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid: Identification of open-reading frames. J. Biol. Chem. 261, 108–121.Google Scholar
  67. Slightom, J. L., Jouanin, L., Leach, F., Drong, R. F., Tepfer, D., 1985: Isolation and identification of TL-DNA/plant junctions in Convolvulus arvensis transformed by Agrobacterium rhizogenes strain A4. EMBO J. 4, 3069–3077.PubMedGoogle Scholar
  68. Spano, L., Costantino, P., 1982: Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on tobacco. Z. Pflanzenphysiol. 106, 87–92.Google Scholar
  69. Spano, L., Pomponi, N., Costantino, P., Van Slogteren, G. M. S., Tempe, J., 1982: Identification of T-DNA in the root-inducing plasmid of the agropine type Agrobacterium rhizogenes 1855. Plant Mol. Biol. 1, 291–300.Google Scholar
  70. Spano, L., Wullems, G. J., Schilperoort, R. A., Costantino, P., 1981: Hairy root: In vitro growth properties of tissues induced by Agrobacterium rhizogenes on tobacco. Plant Sci. Lett. 23, 29–305.Google Scholar
  71. Strobel, G. A., Lam, B., Harrison, L., Hess, B., Lam, S., 1985: Introduction of the hairy root plasmid into Rhizobium meliloti results in increased nodulation on its host. J. Gen. Microbiol. 131, 355–361.Google Scholar
  72. Strobel, G. A., Nachmias, A., 1985: Agrobacterium rhizogenes promotes the initial growth of bare root stock almond. J. Gen. Microbiol. 131, 1245–1249.Google Scholar
  73. Taylor, B. H., Amasino, R. M., White, F. F., Nester, E. W., Gordon, M. P., 1985a: T-DNA analysis of plants regenerated from hairy root tumors. Mol. & Gen. Genet. 201, 554–557.CrossRefGoogle Scholar
  74. Taylor, B. H., White, F. F., Nester, E. W., Gordon, M. P., 1985 b: Transcription of Agrobacterium rhizogenes A4 T-DNA. Mol. & Gen. Genet. 201, 546–553.Google Scholar
  75. Tempe, J., Petit, A., Farrand, S. K., 1984: Induction of cell proliferation by Agrobacterium tumefaciens and A. rhizogenes: A parasite’s point of view. In Plant Gene Research, eds. D.P.S. Verma and Th. Hohn. Wien - New York: Springer, pp. 271–286.Google Scholar
  76. Tepfer, D., 1981: La transformation genetique de plantes supérieures par Agrobacterium rhizogenes. 2e Colloque sur les Recherches Fruitieres - Bordeaux, 47–59.Google Scholar
  77. Tepfer, D., 1984: Transformation of several species of higher plants by Agrobacterium rhizogenes: Sexual transmission of the transformed genotype and phenotype. Cell 37, 959–967.PubMedCrossRefGoogle Scholar
  78. Tepfer, D. A., Tempe, J., 1981: Production d’agropine par des racines formees sous l’action d’Agrobacterium rhizogenes souche A4. CR Acad. Sci. Paris, Ser III 292, 153–156.Google Scholar
  79. Thomashow, L. S., Reeves, S., Thomashow, M. F., 1984: Crown gall oncogenesis: Evidence that a T-DNA gene from the Agrobacterium Ti plasmid PTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. Proc. Natl. Acad. Sei, U.S.A. 81, 5071–5075.CrossRefGoogle Scholar
  80. Thomashow, M. F., Hugly, S., Buchholtz, W. G., Thomashow, L. S., 1986: Molecular basis for the auxin-independent phenotype of crown gall tumor tissue. Science 232, 606–618.Google Scholar
  81. Thomashow, M. F., Nutter, R., Montoya, A. L., Gordon, M. P., Nester, E. W., 1980: Integration and organization of Ti plasmid sequences in crown gall tumors. Cell 19, 729–739.PubMedCrossRefGoogle Scholar
  82. Turgeon, R., Wood, H. N., Braun, A., 1976: Studies on the recovery of crown gall tumor cells. Proc. Natl. Acad. Sei., U.S.A. 73, 3562–3564.CrossRefGoogle Scholar
  83. Unger, L., Ziegler, S. F., Huffman, G. A., Knauf, V. C., Peet, R., Moore, L. W., Gordon, M. P., Nester, E. W., 1985: New class of limited-host-range Agrobacterium mega-tumor-inducing plasmids lacking homology to the transformed DNA of a wide-host-range, tumor-inducing plasmid. J. Bacteriol. 164, 723–730.PubMedGoogle Scholar
  84. White, F. F. Garfinkel, D. J., Huffman, G. A., Gordon, M. P., Nester, E. W., 1983: Sequences homologous to Agrobacterium rhizogenes T-DNA in the genomes of uninfected plants. Nature 301, 348–350.Google Scholar
  85. White, F. F., Ghidossi, G., Gordon, M. P., Nester, E. W., 1982: Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc. Natl. Acad. Sei, U.S.A. 79, 3193–3197.CrossRefGoogle Scholar
  86. White, F. F., Nester, E. W., 1980a: Hairy root: Plasmid encodes virulence traits in Agrobacterium rhizogenes. J. Bacteriol. 141, 1134–1141.PubMedGoogle Scholar
  87. White, F. F., Nester, E. W., 1980b: Relationship of plasmids responsible for hairy root and crown gall tumorigenicity. J. Bacteriol 144, 710–720.PubMedGoogle Scholar
  88. White, F. F., Taylor, B. H., Huffman, G. A., Gordon, M. P., Nester, E. W., 1985: Molecular and genetic analysis of the transferred DNA regions of the root- inducing plasmid of Agrobacterium rhizogenes. J. Bacteriol. 164, 33–44.PubMedGoogle Scholar
  89. Willmitzer, L., Dhaese, P., Schreier, P. H., Schmalenbach, W., Van Montagu, M., Schell, J., 1983: Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors. Cell 32, 1045–1046.PubMedCrossRefGoogle Scholar
  90. Willmitzer, L., Sanchez-Serrano, J., Busfeld, E., Schell, J., 1982: DNA from Agrobacterium rhizogenes is transferred to and expressed in axenic hairy root plant tissues. Mol. & Gen. Genet. 186, 16–22.CrossRefGoogle Scholar
  91. Wullems, G. J., Molendijk, L., Ooms, G., Schilperoort, R. A., 1981: Retention of tumor markers in F-l progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell 24, 719–728.PubMedCrossRefGoogle Scholar
  92. Yang, F., Simpson, R. B., 1981: Revertant seedlings from crown gall tumors retain an portion of the bacterial Ti plasmid DNA sequences. Proc. Natl. Acad. Sei., U.S.A. 78, 4151–4155.CrossRefGoogle Scholar
  93. Yanofsky, M., Montoya, A., Knauf, V., Lowe, B., Gordon, M., Nester, E., 1985: Limited-host-range plasmid of Agrobacterium tumefaciens: Molecular and genetic analyses of transferred DNA. J. Bacteriol. 163, 341–348.PubMedGoogle Scholar
  94. Zambryski, P., Goodman, H., Van Montagu, M., Schell, J., 1983: Agrobacterium tumor induction. In Mobile Genetic Elements, ed. J. Shapiro. Academic Press, pp. 506 - 535.Google Scholar
  95. Zambryski, P., Joos, H., Genetello, C., Leemans, J., Van Montagu, M., Schell, J., 1983: Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 2, 2143–2150.PubMedGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1987

Authors and Affiliations

  • Frank F. White
    • 1
  • Vilas P. Sinkar
    • 2
  1. 1.Department of Plant Pathology, Throckmorton HallKansas State UniversityManhattanUSA
  2. 2.Department of BiochemistryUniversity of WashingtonSeattleUSA

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