• Nigel Grimsley
  • David Bisaro
Part of the Plant Gene Research book series (GENE)


Most plant viruses are transmitted in nature by invertebrate vectors, commonly either insects or nematodes, which have previously fed on infected plants. While it is sometimes desirable to use natural routes of infection in studies in which an understanding of virus ecology or epidemiology is the aim, these methods may be inconvenient for other types of experiments. Vector stocks may be difficult to maintain, and their introduction to plants may be difficult to perform in a controlled manner. It is also impossible to determine the number of virus particles which constitutes the inoculum. Mechanical inoculation of plants with purified virus preparations or isolated viral nucleic acid is often an effective alternative and has the advantage of being quantitative. However, some viruses are intractable in this regard and are mechanically transmitted to their host plants either poorly or not at all. The same is true for the isolated nucleic acid of certain viruses, whether in native or cloned form. The reasons for this are perhaps many, and could include such factors as the inability of the virus or nucleic acid to enter cells, or an inability to replicate in or move through those cell types which are most likely to receive infectious virus particles or nucleic acid during mechanical abrasion of the leaf.


Cucumber Mosaic Virus Cauliflower Mosaic Virus Maize Streak Virus Potato Spindle Tuber Viroid Tomato Golden Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag/Wien 1987

Authors and Affiliations

  • Nigel Grimsley
    • 1
  • David Bisaro
    • 2
  1. 1.Friedrich Miescher-InstitutBasleSwitzerland
  2. 2.Department of Botany and MicrobiologyAuburn UniversityAuburnUSA

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