cDNA Cloning of Plant RNA Viruses and Viroids

  • Pieter Vos
Part of the Plant Gene Research book series (GENE)


After the development of recombinant DNA technology and convenient techniques for rapid determination of nucleotide sequences, the construction of well-defined cloned DNA copies of plant viral RNA genomes and viroids is becoming more and more an integral part of the research on these pathogens. In this chapter I will attempt to show how the availability of such clones indeed has added a new dimension to plant virology.


Mosaic Virus Tobacco Mosaic Virus Strand Synthesis Cucumber Green Mottle Mosaic Virus Potato Spindle Tuber Viroid 
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  1. Ahlquist, P., Janda, H., 1984: cDNA cloning and in vitro transcription of the complete bromo mosaic virus genome. Mol. Cell. Biol., 4, 2876–2882.PubMedGoogle Scholar
  2. Ahlquist, P., French, R., Janda, M., Loesch-Fries, L. S., 1984a: Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc. Natl. Acad. Sci. U.S.A., 81, 7066–7070.PubMedCrossRefGoogle Scholar
  3. Ahlquist, P., Dasgupta, R., Kaesberg, P., 1984 b: Nucleotide sequence of the bromo mosaic virus genome and its implications for viral replication. J. Mol. Biol., 172, 369–383.Google Scholar
  4. Allen, R. N., Dale, J. L., 1981: Application of rapid biochemical methods for detecting avocado sunblotch disease. Ann. Appl. Biol., 98, 451–461.CrossRefGoogle Scholar
  5. Bevan, M. W., Mason, S. E., Goelet, P., 1985: Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium. EMBO J., 4, 1921–1926.PubMedGoogle Scholar
  6. Branch, A. D., Robertson, H. D., Dickson, E., 1981: Longer-than- unit-length viroid minus strands are present in RNA from infected plants. Proc. Natl. Acad. Sci. U.S.A., 78, 6381–6385.PubMedCrossRefGoogle Scholar
  7. Branch, A. D., Robertson, H. D., 1984: A replication cycle for viroids and other small infectious RNAs. Science, 223, 450–455.PubMedCrossRefGoogle Scholar
  8. Bujarski, J. J, Hardy, S. F., Miller, W. A., Hall, T. C., 1982: Use of dodecyl-B-D- maltoside in the purification and stabilisation of RNA polymerase from bromo mosaic virus-infected barley. Virology, 11, 465–473.CrossRefGoogle Scholar
  9. Bujarski, J. J., Dreher, T. W., Hall, T. C., 1985: Deletions in the 3’-terminal tRNA-like structure of bromo mosaic virus RNA differentially affect aminoacylation and replication in vitro. Proc. Natl. Acad. Sci. U.S.A., 82, 5636–5640.PubMedCrossRefGoogle Scholar
  10. Bujarski, J. J., Kaesberg, P., 1986: Genetic recombination between RNA components of a multipartite plant virus. Nature, 321, 528–531.PubMedCrossRefGoogle Scholar
  11. Contreras, R., Cheroute, H., Degrave, W., Fiers, W., 1982: Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes. Nucleic Acids Res., 10, 3133–3142.CrossRefGoogle Scholar
  12. Cress, D. E., Kiefer, M. C., Owens, R. A., 1983: Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Res., 11, 6821–6835.PubMedCrossRefGoogle Scholar
  13. Dasmahapatra, B., Dasgupta, R., Saunders, K., Selling, B., Gallagher, T., Kaesberg, P., 1986: Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus. Proc. Natl. Acad. Sci. U.S.A., 83, 63–66.PubMedCrossRefGoogle Scholar
  14. Daubert, S. D., Bruening, G., Najarian, R. C., 1978: Protein bound to the genome RNAs of cowpea mosaic virus. Eur. J. Biochem., 92, 45–51.PubMedCrossRefGoogle Scholar
  15. Davanloo, P., Rosenberg, A. H., Dunn, J. J., Studier, F. W., 1984: Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A., 81, 2035–2039.PubMedCrossRefGoogle Scholar
  16. Dawson, W. O., Beck, D. L., Knorr, D. A., Grantham, G. L., 1986: cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts. Proc. Natl. Acad. Sci. U.S.A., 83, 1832–1836.Google Scholar
  17. Deutscher, H. P., 1983: Enzymes of nucleic acid synthesis and modification, vol. 2 (Jacob, S. T., ed.) 159–183. CRC Press, Boca Raton, Florida.Google Scholar
  18. Dickson, E., Robertson, H. D., Niblett, C. L., Horst, R. K., Zaitlin, M., 1979: Minor differences between nucleotide sequences of mild and severe strains of potato spindle tuber viroid. Nature, 277, 60–62.CrossRefGoogle Scholar
  19. Diener, T. O., 1983: Viroids. Adv. Virus. Res., 28, 214–283.Google Scholar
  20. Diener, T. O., 1986: Viroid processing: A model involving the central conserved region and hairpin I. Proc. Natl. Acad. Sci. U.S.A., 83, 58–62.PubMedCrossRefGoogle Scholar
  21. Dougherty, W. G., Hiebert, E., 1985: Molecular Plant Virology, Vol. II (Davies, J. W., ed.) 39–42. CRC Press, Boca Raton, Florida.Google Scholar
  22. Dougherty, W. G., Allison, R. F., Parks, T. D., Johnston, R., E., Feild, M. J., Armstrong, F. B., 1985: Nucleotide sequence at the 3’ terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein gene organization. Virology, 146, 282–291.Google Scholar
  23. Dreher, T. W., Bujarski, J. J., Hall, T. C., 1984: Mutant viral RNAs synthesised in vitro show altered aminoacylation and replicase template activities. Nature, 311, 171 - 175.PubMedCrossRefGoogle Scholar
  24. Dunn, J. J., Studier, F. W., 1983: Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. J. Mol. Biol., 166, 477–435.PubMedCrossRefGoogle Scholar
  25. Efstratiadis, A., Kafatos, F. C., Maxam, A. M., Maniatis, T., 1976: Enzymatic in vitro synthesis of globin genes. Cell, 7, 279–288.PubMedCrossRefGoogle Scholar
  26. El Manna, H., Bruening, G., 1973: Polyadenylate sequences in the ribonucleic acids of cowpea mosaic virus. Virology, 56, 198–206.PubMedCrossRefGoogle Scholar
  27. Emini, E. A., Leibowitz, J., Diamond, D. C., Bonin, Wimmer, E., 1984: Recombinants of mahoney and sabin strain poliovirus type 1: analysis of in vitro phenotypic markers and evidence that resistance to guadine maps in the non-structural proteins. Virology, 137, 74–85.PubMedCrossRefGoogle Scholar
  28. Van Emmelo, H., Devos, R., Ysebaert, H., Fiers, W., 1982: Construction and characterisation of a plasmid containing a nearly full-size DNA copy of satellite tobacco necrosis virus RNA. J. Mol. Biol., 143, 259–270.CrossRefGoogle Scholar
  29. Van Emmelo, J., Ameloot, P., Plaetinck, G., Fiers, W., 1984: Controlled synthesis of the coat protein of satellite tobacco necrosis virus in Escherichia coli. Virology, 136, 32–40.PubMedCrossRefGoogle Scholar
  30. Van Emmelo, J., Ameloot, P., Fiers, W. (submitted for publication): Linker insertion mutagenesis of satellite tobacco necrosis virus genome and its expression in plants.Google Scholar
  31. Fernow, K. H., 1967: Tomato as a test plant for detecting mild strains of potato spindle tuber viroid. Phytopathology, 57, 1347–1352.Google Scholar
  32. Fields, S., Winter, G., 1982: Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment. Cell, 28, 303–313.PubMedCrossRefGoogle Scholar
  33. Franssen, H., Goldbach, R., Broekhuysen, H., Moerman, M., Van Kammen, A., 1982: Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. J. Virol., 41, 8–17.PubMedGoogle Scholar
  34. Franssen, H., Goldbach, R., Van Kammen, A., 1984 a: Translation of bottom-component RNA of cowpea mosaic virus in reticulocyte lysate: faithful proteolytic processing of the primary translation product. Virus Research, 1, 39–49.Google Scholar
  35. Franssen, H. J., Moerman, M., Rezelman, G., Goldbach, R., 1984b: Evidence that the 32,000-dalton protein encoded by the bottom-component RNA of cowpea mosaic virus is a proteolytic processing enzyme. J. Virol., 50, 183–190.PubMedGoogle Scholar
  36. Franssen, H., Leunissen, J., Goldbach, R., Lomonossoff, G., Zimmern, D., 1984 c: Homologous sequences in non-structural proteins from cowpea mosaic virus and picorna viruses. EMBO J., 3, 855–861.PubMedGoogle Scholar
  37. French, R., Janda, M., Ahlquist, P., 1986: Bacterial gene inserted in an engineered RNA virus: Efficient expression in monocotyledonous plant cells. Science, 231, 1294 - 1297.PubMedCrossRefGoogle Scholar
  38. French, R., Janda, M., Ahlquist, P., 1986: Bacterial gene inserted in an engineered RNA virus: Efficient expression in monocotyledonous plant cells. Science, 231, 1294 - 1297.PubMedCrossRefGoogle Scholar
  39. Goelet, P., Lomonossoff, G. P., Butler, P. J. G., Akam, M. E., Gait, N. J., Kam, J., 1982: Nucleotide sequence of tobacco mosaic virus RNA. Proc. Natl. Acad. Sei. U.S.A., 79, 5818–5823.CrossRefGoogle Scholar
  40. Goldbach, R. W., Schilthuis, J. G., Van Kammen, A., 1981: Comparison of in vitro translation of cowpea mosaic virus RNAs. Biochem. Biophys. Res. Commun. 99, 89–94.Google Scholar
  41. Goldbach, R. W., Van Kammen, A., 1985: Molecular Plant Virology, Vol. II (Davies, J. W., ed.) 83–120. CRC Press, Boca Raton, Florida.Google Scholar
  42. Green, M., Maniatis, T., and Melton, D., 1983: Human B-globin pre-mRNA synthe- sised in vitro is accurately spliced in Xenopus oocyte nuclei. Cell, 32, 681–694.PubMedCrossRefGoogle Scholar
  43. Grimsley, N., Hohn, B., Hohn, Th., Waiden, R., 1986: “Agroinfection”, an alternative route for viral infection of plants by using the Ti-plasmid. Proc. Natl. Acad. Sei. U.S.A., 83, 3282–3286.CrossRefGoogle Scholar
  44. Harrison, B. D., Robinson, D. J., 1982: Genomic reconstitution and nucleic acid hybridisation as methods of identifying particle-deficient isolates of tobacco rattle virus in potato plan with stem-mottle disease. J. of Virol. Methods, 5, 255–266.CrossRefGoogle Scholar
  45. Henco, K., Sänger, H. L., Riesner, D., 1979: Fine structure melting of viroids as studied by kinetic methods. Nucleic Acids Res., 6, 3041–3059.PubMedCrossRefGoogle Scholar
  46. Jackson, R. J., 1986: A detailed kinetic analysis of the in vitro synthesis and processing of encephalomyocarditis virus products. Virology, 149, 114–127.PubMedCrossRefGoogle Scholar
  47. Van Kammen, A., 1985: The replication of plant virus RNA. Microbiological Sciences, 2, 170–174.PubMedGoogle Scholar
  48. Keese, P., Symons, R. H., 1985: Domains in viroids: Evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proc. Natl. Acad. Sei. U.S.A., 82, 4582–4586.CrossRefGoogle Scholar
  49. Kiberstis, P. A., Hall, T. C., 1983: N-acetyl-tyrosine at the 3’ end of bromo mosaic virus RNA has little effect on infectivity. J. Gen. Virol., 64, 2073–2077.CrossRefGoogle Scholar
  50. King, A. M. Q., McCahon, D., Slade, W. R., Newman, J. W. I., 1982: Biochemical evidence of recombination within the unsegmented RNA genome of aph- tovirus. J. Virol., 41, 66–77.PubMedGoogle Scholar
  51. Lane, L., Kaesberg, P., 1971: Biophysically homogenous bromegrass mosaic virus contains multiple genetic components. Nature, 232, 40–43.CrossRefGoogle Scholar
  52. Lane, L., 1981: Handbook of plant virus infections and comparative diagnosis (Kurstak, E., ed.) 333–376. Elsevier North Holland Biomedical Press, Amsterdam.Google Scholar
  53. Leary, J. L., Brigati D. J., Word, D. C., 1981: Rapid and sensitive colorimetric method for visualising biotin-labeled DNA probes hybridised to DNA or RNA immobilised on nitrocellulose: Bio-blots. Proc. Natl. Acad. Sei. U.S.A., 80, 4045–4059.CrossRefGoogle Scholar
  54. Loesch-Fries, L. S., Hall, T. C., 1982: In vivo aminoacylation of bromo mosaic and barley strip mosaic virus RNAs. Nature, 298, 771–773.CrossRefGoogle Scholar
  55. Lomonossoff, G., Shanks, M., 1983: The nucleotide sequence of cowpea mosaic virus B RNA. EMBO J., 2, 2253–2258.PubMedGoogle Scholar
  56. Maule, A. J., Hull, R., Donson, J., 1983: The application of spot hybridisation to the detection of DNA and RNA viruses in plant tissues. J. of Virol. Methods, 6, 215–224.CrossRefGoogle Scholar
  57. Maxam, A. M., Gilbert, W., 1980: In: Methods in Enzymology, 65, 499–560 ( Grossman, L., Moldave, K, eds.). Academic Press, Inc., New York.Google Scholar
  58. McDonnell, M. W., Simon, H. N., Studier, F. W., 1977: Analysis of restriction fragments of T7 DNA and determination of molecular weights in neutral and alkaline gels. J. Mol. Biol., 110, 119–146.CrossRefGoogle Scholar
  59. McKinney, H. H., 1929: Mosaic diseases in the Canary Islands, West Africa and Gibraltar. J. Agric. Res., 39, 557–566.Google Scholar
  60. Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., Green, M. R., 1984: Efficient in vitro synthesis of biologically active RNA and RNA hybridisation probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res., 12, 7035–7056.PubMedCrossRefGoogle Scholar
  61. Meshi, T, Kiyanna, R, Ohno, T, Okada, Y., 1983: Nucleotide sequence of the coat protein cistron and the 3’ non-coding region of cucumber green mottle mosaic virus (watermelon strain) RNA. Virology, 127, 54–64.PubMedCrossRefGoogle Scholar
  62. Meshi, T., Ishikama, H., Watamabe, Y., Yamaya, H., Okada, Y., Sano, T., Shikata, E., 1985: The sequence necessary for the infectivity of hop stunt viroid cDNA clones. Mol. Gen. Genet., 200, 199–206.CrossRefGoogle Scholar
  63. Meshi, T., Ishikawa, M., Motoyoshi, F., Semba, K., Okada, Y., 1986:In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus. Proc. Natl. Acad. Sci. U.S.A., 83, 5043–5047.Google Scholar
  64. Miller, W. A., Bujarski, J. J., Dreher, T. W., Hall, T. C., 1986: Minusstrand initiation by bromo mosaic virus replicase within the 3’ tRNA-like structure of native and modified RNA templates. J. Mol. Biol., 187, 537–546.PubMedCrossRefGoogle Scholar
  65. Miller, W. A., Hall, T. C., 1983: Use of micrococcal nuclease in the purification of highly template dependent RNA-dependent RNA polymerase from brome mosaic virus-infected barley. Virology, 125, 236–241.PubMedCrossRefGoogle Scholar
  66. Miller, W. A., Dreher, T. A., Hall, T. C., (1985): Synthesis of bromo mosaic virus subgenomic RNA in vitro initiation on (-) sense genomic RNA. Nature, 313, 68–70.PubMedCrossRefGoogle Scholar
  67. Mizutani, S., Colonno, R. J., 1985:In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14. J. Virol., 56, 628–632.Google Scholar
  68. Murant, A. F., Mayo, M. A., 1982: Satellites of plant viruses. Ann. Rev. Phytopathol., 20, 49–70.CrossRefGoogle Scholar
  69. Okayama, H., Berg, P., 1982: High-efficiency cloning of full-length cDNA. Mol. Cell. Biol, 2, 161–170.PubMedGoogle Scholar
  70. Ohno, T, Takamatsu, N, Meshi, T, Okado, Y, 1982: Hop shunt viroid: Molecular cloning and nucleotide sequence of the complementary cDNA copy. Nucleic Acids Res, 11, 6180–6197.Google Scholar
  71. Owens, R. A, Diener, T. O, 1981: Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic acid hybridization. Science, 213, 670–672.PubMedCrossRefGoogle Scholar
  72. Owens, R. A, Diener, T. O, 1982: RNA intermediates in potato spindle tuber in viroid replication. Proc. Natl. Acad. Sci. U.S.A., 79, 113–117.PubMedCrossRefGoogle Scholar
  73. Owens, R. A, Diener, T. O, 1984: Spot hybridisation for detection of viroids and viruses. In: Methods in Virology, vol. VII ( Maramorosch, K, Koprowski, H, eds.). Academic Press Inc., New York.Google Scholar
  74. Palukaitis, P, Rakowski, A. G, Alexander, D, McE, Symons, R. H, 1981: Rapid indexing of the sunblotch disease of avocados using a complementary DNA probe to avocado sunblotch viroid. Ann. Appl. Biol, 98, 439–449.Google Scholar
  75. Palukaitis, P, Cotts, S, Zaitlin, M, 1985: Detection and identification of viroids and viral nucleic acids by dot-blot hybridisation. Acta Horticulturae, 164, 109–118.Google Scholar
  76. Pelham, H. R. B, 1978: Translation of encephalomyocarditis virus RNA in vitro yields an active proteolytic processing enzyme. Eur. J. Biochem, 85, 457–462.PubMedCrossRefGoogle Scholar
  77. Plotch, S, J, Bouloy, M, Ulmanen, I, Krug, R. H, 1981: A unique cap (m7GpppXm)-dependent influenza virion endonuclease cleaves capped RNAs to generate the primer that initiate viral RNA transcription. Cell, 23, 847–858.PubMedCrossRefGoogle Scholar
  78. Putnak, J. R, Philips, B. A, 1981: Picornaviral structure and assembly. Microbiol. Rev, 45, 287–315.PubMedGoogle Scholar
  79. Queen, C, 1983: A vector that uses phage signals for efficient synthesis of proteins in Escherichia coli. J. Mol. Appl. Genet, 2, 1–10.Google Scholar
  80. Racaniello, V. R., Baltimore, D., 1981: Cloned poliovirus complementary DNA is infectious in mammalian cells. Science, 214, 916–919.PubMedCrossRefGoogle Scholar
  81. Retzel, E. F., Collet, M. S., Faras, A. J., 1980: Enzymatic synthesis of deoxyribonucleic acid by the avian retrovirus reverse transcriptase in vitro: optimum conditions required for transcription of large ribonucleic acid templates. Biochemistry, 19, 513–518.PubMedCrossRefGoogle Scholar
  82. Rezelman, G., Goldbach, R., Van Kammen, A., 1980: Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts. J. Virol., 36, 366–373.Google Scholar
  83. Rogers, S. G., Bisaro, D. H., Horsch, R. B., Fraley, R. T., Hoffmann, N. L., Brand, L., Elmer, J. S., Lloyd, A. H., 1986: Tomato golden mosaic virus A component DNA replicates autonomously in transgenic plants. Cell, 45, 593–600.PubMedCrossRefGoogle Scholar
  84. Sano, T., Uyeda, I., Shikata, E., Ohno, T., Okada, Y., 1984: Nucleotide sequence of cucumber pale fruit viroid: homology to hop stunt viroid. Nucleic Acids Res., 12, 3427–3434.PubMedCrossRefGoogle Scholar
  85. Riesner, D., Henco, K., Rokol, U., Klotz, G., Kleinschmidt, A. K., Gross, H. J., Domdey, H., Sänger, H. L., 1979: Structure and structure formation of viroids. J. Mol. Biol., 133, 85–15.PubMedCrossRefGoogle Scholar
  86. Sänger, H. L., 1984: In: The Microbe 1984: Part I Viruses, 281 - 334 (Mahy, B. W. J., Pattison, J. R., eds.). Cambridge University Press, Cambridge, U. K.Google Scholar
  87. Schuhmacher, H., Randies, J. W., Riesner, d., 1983: a two-dimensional electro- phoretic technique for the detection of circular viroids and virusoids. Anal. Biochem., 135, 288–295.Google Scholar
  88. Schnölzer, M., Haas, B., Ramm, K., Hoffman, H., Sänger, H. L., 1985: Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTV). EMBO J., 3, 2181–2190.Google Scholar
  89. Sela, I., Riechman, H., Weissbach, A., 1982: Comparison of dot molecular hybridisation and enzyme linked immunosorbent assay for detecting tobacco mosaic virus in plant tissue and protoplasts. Phytopathology, 74, 385–389.CrossRefGoogle Scholar
  90. Sippel, A. E., 1973: Pruification and characterisation of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli. Eur. J. Biochem., 37, 31.PubMedCrossRefGoogle Scholar
  91. Stanley, J., Rottier, R., Davies, J. W., Zabel, P., Van Kammen, A., 1978: A protein linked to the 5-termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Res., 5, 4505–4522.Google Scholar
  92. Symons, R. H., 1985: Diagnostic approaches for the rapid and specific detection of plant viruses and viroids. In: Plant Microbe Interactions, Molecular and Genetic Perspectives, vol. I, 93–124 ( Kosuge, T. and Nester, E. W., eds.). Macmillan Publishing Co., New York.Google Scholar
  93. Tabler, M., Sänger, H. L., 1984: Cloned single- and double-stranded DNA copies of potato spindle tuber viroid (PSTV) RNA and co-inoculated subgenomic DNA fragments are infectious. EMBO J., 3, 3055–3062.PubMedGoogle Scholar
  94. Tabor, S., Richardson, C. C., 1985, A bacteriophage T7 RNA polymerase/ promotor system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sei. U.S.A., 82, 1074–1078.CrossRefGoogle Scholar
  95. Taniguchi, T., Palmieri, M., Weissmann, C., 1978: QB DNA-containing hybrid Plasmids giving rise to QB phage formation in the bacterial host. Nature, 274, 223–228.PubMedCrossRefGoogle Scholar
  96. Vance, V. B., Beachy, R. N., 1984: Translation of soybean mosaic virus RNA in vitro: evidence of protein processing. Virology, 132, 271–281.PubMedCrossRefGoogle Scholar
  97. Verver, J., Goldbach, R., Garcia, J., Vos, P., submitted for publication.In vitroexpression of a full-length DNA copy of cowpea mosaic virus B RNA: Identification of the B RNA-encoded 24-kilodalton protein as a viral protease.Google Scholar
  98. Visvader, J. E, Symons, R. N., 1985: Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucleic Acids Res., 13, 2907–2920.PubMedCrossRefGoogle Scholar
  99. Vos, P., Verver, J., Van Wezenbeek, P., van Kammen, A., Goldbach, R., 1984: Study of the genetic organisation of a plant viral genome by in vitro expression of a full-length DNA copy. EMBO J., 3, 3049 - 3053.Google Scholar
  100. Wellink, J., Rezelman, G., Goldbach, R, Beyreuther, K., 1986: Determination of the proteolytic processing sites in the polyprotein encoded by the bottom-com- ponent RNA of cowpea mosaic virus. J. Virol., 60, 50–58.Google Scholar
  101. Van der Werf, S., Bradley, J., Wimmer, E., Studier, F. W., Dunn, J. J., 1986: Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc. Natl. Acad. Sei. U.S.A., 83, 2330–2334.CrossRefGoogle Scholar
  102. Van Wezenbeek, P., Vos, P., Van Boom, J., Van Kammen, A., 1982: Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA. Nucleic Acids Res., 10, 7947–7957.PubMedCrossRefGoogle Scholar
  103. Van Wezenbeek, P., Verver, J., Harmsen, J., Vos, P., Van Kammen, A., 1983: Primary structure and gene organization of the middle component RNA of cowpea mosaic virus. EMBO J., 2, 941–946.PubMedGoogle Scholar
  104. Ysebaert, N, Van Emmelo, J., Fiers, W., 1980: Total nucleotide sequence of a nearly full-size DNA copy of satellite tobacco necrosis virus RNA. J. Mol. Biol, 143, 273–287.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1987

Authors and Affiliations

  • Pieter Vos
    • 1
  1. 1.Department of Molecular BiologyAgricultural UniversityWageningenThe Netherlands

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