Abstract
A great deal of progress has been achieved during the past years in the transformation of higher plants. In spite of the impressive advances in this field, a major drawback remains the slow growth of these organisms. In most cases it still takes several weeks or even months to regenerate a plant. While higher plants are uniquely suited for developmental studies, lower plants, especially unicellular algae, offer attractive possibilities for investigating basic features of plants such as photosynthesis and chloroplastnucleocytoplasmic interactions. These unicellular organisms grow rapidly and some of them can be manipulated with ease both at the biochemical and genetic level. However, transformation of lower plants is still in its infancy and only limited success has been reported. This article will provide a progress report on the transformation of Chlamydomonas reinhardtii. A short review on this transformation system was published earlier (Rochaix, 1983).
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References
Blanc, H., Dujon, B., 1981: Replication regions of the yeast mitochondrial DNA active in vitro and in yeast transformants. In: Slonimski etal. (eds.), Mitochondrial Genes, Cold Spring Harbor Laboratory Press N. Y., pp. 279–294.
Beach, D., Piper, M., Shall, S., 1980: Isolation of chromosomal origins of repli¬cation in yeast. Nature 284, 185–187.
Bennoun, P., Diner, B. A., Wollman, F. A., Schmidt, G., Chua, N. H., 1981: Thyla- koid polypeptides associated with photosystem II in Chlamydomonas reinhardtii: comparison of system II mutants and particles. In: Akoyunoglou, G. (ed.), Photosynthesis III. Structure and Molecular Organisation of the Photo- synthetic apparatus. Balaban International Science Services, Philadelphia, PA, pp. 839–849.
Bennoun, P., Spierer-Herz, M., Erickson, J., Girard-Bascou, J., Pierre, Y., Delosme, M., Rochaix, J.-D., 1986: Characterization of photosystem II mutants of Chlamydomonas reinhardtii lacking the psbA gene. Plant Molec. Biol. 6, 141–160.
Bergmann, P., Schneider, M., Burkard, G., Weil, J. H., Rochaix, J.-D., 1985: Transfer RNA Gene Mapping Studies on Chloroplast DNA from Chlamy¬domonas reinhardtii. Plant Science 39, 133–140.
Broach, J. R., Li, Y. Y., Feldman, J., Jayaram, M., Abraham, J., Nasmyth, K. A., Hicks, J. B., 1980: Localization and Sequence Analysis of Yeast Origins of DNA replication. Cold Spring Harbor Symp. Quant. Biol. 47, 1165–1173.
Brunke, K. J., Young, E. E., Buchbinder, B. U., Weeks, D. P., 1982: Coordinate regulation of the four tubulin genes of Chlamydomonas reinhardtii. Nucl. Acids Res. 10, 1295–1310.
Celniker, S. E., Campbell, J. L., 1982: Yeast DNA replication in vitro: Initiation and Elongation Events Mimic in Vivo Processes. Cell 31, 201–203.
Chan, C. S. M., Tye, B. R. K., 1980: Autonomously replicating sequences in Sac- charomyces cerevisiae. Proc. Natl. Acad. Sci., U.S.A. 77, 6329–6333.
Claes, H., 1971: Autolysin of the cell wall from the gametes of Chlamydomonas reinhardtii. Arch, für Mikrobiologie 78, 180–188.
Clarke, L., Carbon, J., 1978: Functional Expression of Cloned Yeast DNA in Escherichia coli: Specific Complementation of Argininosuccinate Lyase (argH) Mutations. J. Mol. Biol. 120, 517–532.
Cox, J. C., Bingham, S. E., Bishop, R. J., 1985: Transformation of Chlamydomonas reinhardtii with plasmids conferring antibiotic (Kanamycin) resistance. Abstracts 1st Intl. Congr. Plant Molec. Biol. p. 115.
Davies, D. R., Plaskitt, A., 1971: Genetical and Structural Analysis of Cell Wall formation in Chlamydomonas reinhardtii. Genet. Res. 17, 33–43.
De Block, M., Schell, J., Van Montagu, M., 1985: Chloroplast transformation by Agrobacterium tumefaciens. EMBO J. 4, 1367–1372.
Dron, M., Rahire, M., Rochaix, J.-D., Mets, L., 1983: First DNA Sequence of a Chloroplast Mutation. A Missense Alteration in the Ribulosebisphosphate Car-boxylase Large Subunit Gene. Plasmid 9, 32–324.
Erickson, J. M., Rahire, M., Rochaix, J.-D., 1984: Chlamydomonas reinhardtii gens for the 32000 mol wt protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat. EMBO J. 3, 2753–2762.
Erickson, J. M., Rahire, M., Rochaix, J.-D., Mets, L., 1985: Herbicide Resistance and Cross-Resistance: Changes at three distinct Sites in the Herbicide-Binding Protein. Science 228, 204–207.
Erickson, J. M., Rahire, M., Malnoe, P., Girard-Bascou, J., Pierre, Y., Bennoun, P., Rochaix, J.-D., 1986: Lack of the D2 protein in a Chlamydomonas reinhardtii psbD mutant affects photosystem II stability and D1 expression. EMBO J., submitted.
Ferris, P. J., Vogt, V. M., 1982: Structure of the Central Spacer Region of Extra- chromosomal Ribosomal DNA in Physarum polycephalum. J. Mol. Biol. 159, 359–381.
Galloway, R., Mets, L., 1984: Atrazine, bromacil and diuron resistance in Chlamy-domonas: A single non-mendelian genetic locus controls the structure of the thylakoid binding site. Plant Physiol. 74, 469–474.
Gillham, N. W., 1965: Induction of chromosomal and non chromosomal mutations in Chlamydomonas reinhardtii with N-methyl-N’-Nitro-N-Nitrosoguanidine. Genetics 52, 529–537.
Gillham, N. W., 1978: Organelle Heredity, Raven, New York.
Goldschmidt-Clermont, M ., 1986: The two genes for the small subunit of RuBP Carboxylase/oxygenase are closely linked in Chlamydomonas reinhardtii. Plant Molec. Biol. 6, 13–21.
Harris, E. H., 1982: Nuclear gene Loci of Chlamydomonas reinhardtii. In: O’Brien, S. J. (ed.), Genetic Maps, Vol. 2, pp. 168–174, Laboratory of Viral Carcino-genesis, National Cancer Institute, NIH, Frederick, MD 21701.
Hasnain, S. E., Manavathu, E. K., Leung, W. L., 1985: DNA mediated Transformation of Chlamydomonas reinhardtii cells: Use of Aminoglycoside 3’–phos-photransferase as a selectable marker. Mol. Cell Biol. 5, 3647–3650.
Hsiao, C., Carbon, J. M., 1979: High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene. Proc. Natl. Acad. Sci., U.S.A. 76, 3829–3833.
Hyman, B. C., Cramer, J. H., Rownd, R. H., 1982: Properties of a Saccharomyces cerevisiae mtDNA segment conferring high-frequency yeast transformation. Proc. Natl. Acad. Sci., U.S.A. 79, 1578–1582.
Koyo, H., Greenberg, B. D., Sugino, A., 1981: Yeast 2 μ plasmid DNA replication in vitro: Origin and direction. Proc. Natl. Acad. Sci., U.S.A. 78, 7261–7265.
Künzler, P., 1985: The linear extrachromosomal DNA of Physarum polycephalum replicates and is maintained under non selective conditions in two different lower eukaryotes. Nucl. Acids Res. 13, 1855–1869.
Lemieux, C., Turmel, M., Lee, R. W., 1980: Characterization of chloroplast DNA in Chlamydomonas eugametes and C. moewusii and its inheritance in hybrid progeny. Curr. Genet. 2, 139–147.
Loppes, R., Matagne, R., Strijkert, P. J., 1972: Complementation at the ARG7 locus in Chlamydomonas reinhardtii. Heredity 28, 239–251.
Loppes, R., Matagne, R., 1972: Allelic complementation between ARG7 mutants in Chlamydomonas reinhardtii. Genetica [The Hague] 43, 422–430.
Loppes, R., Denis, C., 1983: Chloroplast and Nuclear DNA Fragments from Chlamydomonas Promoting High Frequency Transformation of Yeast. Curr. Genet. 7, 473–480.
Mayfield, S. P., Bennoun, P., Rochaix, J.-D., 1987 a: Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photo- system II particles in Chlamydomonas reinhardtii. EMBO J. 6, 313–318.
Mayfield, S. P., Bennoun, P., Rochaix, J.-D., 1987a: Expression of the nuclear encoded OEE1 protein is required for oxygen evolution and stability of photo- system II particles in Chlamydomonas reinhardtii. EMBO J. 6, 313–318.
Mets, L., 1980: Uniparental inheritance of chloroplast DNA sequences in interspe¬cific hybrids of Chlamydomonas. Curr. Genet. 2, 131–138.
Myers, A. M., Grant, D. M, Rabert, D. K., Harris, E. H., Boynton, J. E., Gillham, N. W., 1982: Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7, 133–151.
Newlon, C. S., Burke, W., 1980: Replication of small chromosomal DNAs in yeast. In: Alberts, B., Fox, C. C. (eds.), ICN-UCLA Symposia on Molecular Cellular Biology 19B; pp. 339–409, Academic Press, N. Y.
Newlon, C. S., Devenish, R. J., Suci, P. A., Roffis, C. J., 1981: Replication origins used in Vivo in yeast. In: Rays, D. S., Fox, C. C. (eds.), ICN-UCLA Symposia on Molecular and Cellular Biology 22, pp. 501–506, Academic Press, N. Y.
Rochaix, J.-D., 1978: Restriction Endonuclease Map of the Chloroplast DNA of Chlamydomonas reinhardtii. J. Mol. Biol. 126, 597–617.
Rochaix, J.-D., 1981: Organization, function and expression of the chloroplast DNA of Chlamydomonas reinhardtii. Experientia 37, 323–332.
Rochaix, J.-D ., 1983: Transformation in the green Alga Chlamydomonas reinhardtii. In: Genetic Engineering Vol. 6. Setlow, J. K. and Hollaender, A. (eds.), Plenum Press, New York - London.
Rochaix, J.-D., Malnoe, P. M., 1978: Anatomy of the chloroplast ribosomal DNA of Chlamydomonas reinhardtii. Cell 15, 661–670.
Rochaix, J.-D., van Dillewijn, J., 1982: Transformation of the green alga Chlamy¬domonas reinhardtii with yeast DNA. Nature 296, 70–72.
Rochaix, J.-D., Dron, M., Schneider, M., Vallet, J. M., Erickson, J., 1983: Chlamy¬domonas reinhardtii: A model system for studying the biosynthesis of the photo- synthetic apparatus. Downey etal. (eds.). In: Advances in Gene Technology: Molecular Genetics of Plants and Animals, Miami Winter Symposia, vol. 20, pp. 81–100, Academic Press.
Rochaix, J.-D., van Dillewijn, J., Rahire, M., 1984: Construction and Characteri¬zation of Autonomously Replicating Plasmids in the Green Unicellular Alga Chlamydomonas reinhardtii. Cell 36, 925–931.
Sheperd, H. S., Ledoigt, G., Howell, S. H., 1983: Regulation of light-harvesting chlorophyll-binding protein (LHCP) mRNA accumulation during the cell cycle in Chlamydomonas reinhardtii. Cell 32, 99–107.
Silflow, C. D., Rosenbaum, J. L., 1981f: Multiple a and ß tubulin genes in Chlamy-domonas and regulation of tubulin mRNS levels after deflagellation. Cell 24, 81–88.
Spreitzer, R. J., Mets, L., 1980: Non-Mendelian mutation affecting ribulose 1,5-bis- phosphate carboxylase structure and activity. Nature 285, 114–115.
Spreitzer, R. J., Ogren, W. L., 1983: Rapid recovery of chloroplast mutations affecting ribulosebisphosphate carboxylase/oxygenase in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., U.S.A. 80, 6293–6297.
Spreitzer, R. J., Goldschmidt-Clermont, M., Rahire, M., Rochaix, J.-D., 1985: Non-sense mutations in the Chlamydomonas chloroplast gene that codes for the large subunit of ribulose bisphosphate carboxylase/oxygenase. Proc. Natl. Acad. Sci., U.S.A. 82, 5460 - 5464.
Stern, D. B., Lonsdale, D. M., 1982: Mitochondrial and chloroplast genomes of maize have a 12 kb DNA sequence in common. Nature 299, 698–702.
Stinchcomb, D. T., Struhl, K., Davis, R. W., 1979: Isolation and characterization of a yeast chromosomal replicator. Nature 282, 39–43.
Struhl, K., Stinchcomb, D. T., Scherer, S., Davis, R. W., 1979: High frequency trans-formation of yeast: autonomous replication of hybrid DNA molecules. Proc. Natl. Acad. Sci., U.S.A. 76, 1035–1079.
Timmis, J. N., Scott, N. S., 1983: Sequence homology between spinach nuclear and chloroplast genomes. Nature 305, 65–67.
Tudzynski, P., Esser, K., 1983: Nuclear Association in Yeast of a Hybrid Vector Containing Mitochondrial DNA. Curr. Genet. 7, 165–166.
Turmel, M., Bellemare, G., Lee, R. W., Lemieux, C., 1986: A linear DNA molecule of 5.9 kb is highly homologous to the chloroplast DNA in the green alga Chlamydomonas moewusii. Plant Molec. Biol. 6, 313–319.
Vallet, J. M., Rahire, M., Rochaix, J.-D., 1984: Localization and sequence analysis of chloroplast DNA sequences of Chlamydomonas reinhardtii that promote autonomous replication in yeast. EMBO J. 3, 415–421.
Vallet, J. M., Rochaix, J.-D., 1985: Chloroplast origins of DNA replications are dis¬tinct from chloroplast ARS sequences in two green algae. Curr. Genet. 9, 321–324.
Waddell, J., Wang, X.-M., Wu, M., 1984: Electron microscopic localization of the chloroplast DNA replicative origins in Chlamydomonas reinhardtii. Nucl. Acids Res. 12, 3842–3856.
Wang, X.-M., Chang, C. H., Waddell, J., Wu, M., 1984: Cloning and delimiting one chloroplast DNA replicative origin of Chlamydomonas.
Watson, J. C., Surzycki, S. J., 1982: Extensive sequence homolgy in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast. Proc. Natl. Acad. Sci., U.S.A. 79, 2264–2267.
Watson, J., Surzycki, S. J., 1983: Both the chloroplast and Nuclear genomes of Chlamydomonas reinhardtii share Homology with Escherichia coli Genes for Transcription and Translational components/Current Genet. 7, 201–210.
Woessner, J. P., Masson, A., Harris, E. H., Bennoun, P., Gillham, N. W., Boynton, J. E., 1984: Molecular and genetic analysis of the chloroplast ATPase of Chlamydomonas. Plant Molec. Biol. 3, 177–190.
Youngblom, J., Schloss, J. A., Silflow, C. D., 1984: The two ß tubulin genes of Chlamydomonas reinhardtii Code for identical Proteins. Mol. Cell Biol. 4, 2682–2696.
Zakian, V., 1981: Origin of replication from Xenopus laevis mitochondrial DNA promotes high-frequency transformation of yeast. Proc. Natl. Acad. Sci., U.S.A. 78, 3128–3132. Zakian, V., Kupfer, D. M., 1982: Replication and Segregation of an Unstable Plasmid in Yeast. Plasmid 8, 15–28.
Zakian, V., Kupler, D. M., 1982: Replication and Segregation of an Unstable Plasmid in Yeast. Plasmid 8, 15–28.
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Rochaix, JD. (1987). Transformation of Chlamydomonas Reinhardtii . In: Hohn, T., Schell, J. (eds) Plant DNA Infectious Agents. Plant Gene Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6977-3_11
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DOI: https://doi.org/10.1007/978-3-7091-6977-3_11
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