Abstract
The ability to integrate functional genes stably into a plant genome not only offers a powerful approach to address the fundamental questions of developmental gene expression but also provides valuable opportunities for crop improvement (for reviews, see Fraley et al., 1986; Goodman et al, 1987). Exciting progress has been made during the last four years in the identification and transfer of genes that confer resistance to plant viruses and insect pests. Gene transfer has also been used to engineer resistance to nonselective, environmentally safe herbicides. Over the past several years, the use of herbicides has become an established practice in world agriculture. By eliminating weeds that compete with crops for water and nutrients, herbicides increase the crop yield. New highly potent herbicides have been developed that inhibit plant growth by interfering with the biosynthesis of essential amino acids, rather than by inactivating a component of the photosynthetic apparatus (Table 1) (LaRossa and Falco, 1984). These structurally unrelated herbicides include: glyphosate which inhibits the synthesis of aromatic amino acids; the sulfonylurea and imidazolinone herbicides which block branched chain amino acid biosynthesis; and phosphinothricin which inhibits glutamine biosynthesis. Although potent and environmentally safe, these herbicides have broad-spectrum activity that discriminates poorly between weeds and crops. The genetic engineering of selective resistance to these herbicides in crop species will have substantial agronomic significance and has been the major focus of research in several labs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Amrhein, N., Johanning, D., Smart, G. C., 1985: A glyphosate-tolerant plant tissue culture. In: Primary and Secondary Metabolism of Plant Cell Cultures. Neumann, K. H. (ed.), pp. 356–361, Berlin, Springer-Verlag.
Anderson, P. C., Georgeson, M., 1986: Selection and characterization of imidazolinone tolerant mutants of maize. In: The Biochemical Basis of Herbicide Action. Twenty-seventh Harden Conference Programme and Abstracts. Wye College, Ashford, United Kingdom.
Bayer, E., Gugel, K. H., Hagele, K., Hagemaier, H., Jessipow, S., Konig, W. A., Zahner, Z., 1972: Stoffwechselprodukte von Mikroorganismen, 98. Mitteilung — Phosphinothricin and phosphinothricyl-alanyl-alanin. Helv. Chim. Acta 55, 224–239.
Chaleff, R. S., Mauvais, C. J., 1984: Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224, 1443–1445.
Chaleff, R. S., Ray, T. B., 1984: Herbicide-resistant mutants from tobacco cell cultures. Science 223, 1148–1151.
Charles, I. G, Keyte, J. W., Brammar, W. J., Smith, M., Hawkins, A. R., 1986: The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans. Nucleic Acids Research 14, 2201–2213.
Colanduoni, J. A., Villafranca, J. J., 1986: Inhibiton of Escherichia coli glutamine synthetase by phosphinothricin. Bioorg. Chem. 14, 163–169.
Cornai, L., Sen, L. C., Stalker, D. M., 1983: An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221, 370–371.
Cornai, L., Facciotti, D., Hiatt, W. R., Thompson, G., Rose, R. E., Stalker, D. M., 1985: Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317, 741–744.
DasSarma, S., Tischer, E., Goodman, H. M., 1986: Plant glutamine synthetase complements a glnA mutation in Escherichia coli. Science 232, 1242–1244.
della-Cioppa, G., Bauer, S. C., Klein, B. K., Shah, D. M., Fraley, R. T, Kishore, G. M., 1986: Translocation of the precursor of 5-enolpyruvylshikimate-3-phos-phate synthase into chloroplasts of higher plants in vitro. Proc. Natl. Acad. Sci. U.S.A. 83, 6873–6877.
della-Cioppa, G., Bauer, S. C., Taylor, M. L., Rochester, D. E., Klein, B. K., Shah, D. M., Fraley, R. T., Kishore, G. M., 1987: Targeting a herbicide-resistant enzyme from Escherichia colilo chloroplasts of higher plants. Bio/technology 5, 579–584.
Donn, G., Tischer, E., Smith, J. A., Goodman, H. M., 1984: Herbicide-resistant alfalfa cells: an example of gene amplification in plants. J. Mol. Appl. Genet. 2, 621–635.
Duncan, K., Lewendon, A., Coggins, J. R., 1984: The complete amino acid sequence of Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase. FEBS Lett. 170, 59–63.
Falco, S. C., Dumas, K. S., 1985: Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl. Genetics 109, 21–35.
Fillatti, J. J., Kiser, J., Rose, R., Cornai, L., 1987: Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/technology, in press.
Fraley, R. T., Rogers, S. G., Horsch, R. B., 1986: Genetic transformation in higher plants. CRC Crit. Rev. in Plant Sci. 4, 1–46.
Goodman, R. M., Hauptli, H., Crossway, A., Knauf, V. C., 1987: Gene transfer in crop improvement. Science 236, 48–54.
Haughn, G. W., Somerville, C., 1986: Sulfonylurea resistant mutants of Arabidopsis thaliana. Mol. Gen. Genet. 204, 430–434.
Jones, A. V., Young, R. M., Leto, K., 1985: Subcellular localization and properties of acetolactate synthase, target/site of the sulfonylurea herbicides. Plant Physiol. 77, S293.
Kishore, G. M., Brundage, L., Kolk, K., Padgette, S. R., Rochester, D., Huynh, K., della-Cioppa, G., 1986: Isolation, purification and characterization of a glyphosate tolerant mutant E. coli EPSP synthase. Fed. Proc. 45, 1506.
LaRossa, R. A., Falco, S. C., 1984: Amino acid biosynthetic enzymes as targets of herbicide action. Trends in Biotech. 2, 158–161.
LaRossa, R. A., Schloss, J. V., 1984: The sulfonylurea herbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthase in Salmonella typhimurium. J. Biol. Chem. 259, 8753–8757.
LaRossa, R. A., Smulski, D. R., 1984: ilvB-encoded acetolactate synthase is resistant to the herbicide sulfometuron methyl. J. Bacteriol. 160, 391–394.
Leason, M., Cunliffe, D., Parkin, D., Lea, P. J., Miflin, B. J., 1982: Inhibition of pea glutamine synthetase by methionine-sulfoxamine, phosphinothricin and other glutamate analogues. Phytochem. 21, 855–857.
Levitt, G., Ploeg, H. L., Weigel, R. C., Fitzgerald, D. J., 1981: 2-chloro-N-[4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino-carbonyl] benzenesulfonamide, a new herbicide. J. Agric. Food. Chem. 29, 416–424.
Manderscheid, R., Wild, A., 1986: Studies on the mechanism of inhibition by phosphinothricin of glutamine synthetase isolated from Triticum aestivum L. J. Plant Physiol. 123, 135–142.
Margulis, L., 1970: Origin of Eukaryotic Cells. Yale University Press, New Haven, Connecticut.
Miflin, B. J., Lea, P. J., 1977: Amino acid metabolism. Ann. Rev. Plant Physiol. 28, 299–329.
Mousdale, D. M., Coggins, J. R., 1984: Purification and properties of 5-enolpyru-vylshikimate-3-phosphate synthase from seedlings of Pisum sativum L. Planta 160, 78–83.
Mousdale, D. M., Coggins, J. R., 1985: Subcellular localization of the common shikimate pathway enzymes in Pusum sativum L. Planta 163, 241–249.
Nafziger, E. D., Widholm, J. M., Steinrucken, H. C., Kilmer, J. L., 1984: Selection and characterization of a carrot cell line tolerant to glyphosate. Plant Physiol. 76, 571–574.
Orwick, P. L., Marc, P. A., Umeda, K., Shaner, D. L., Los, M., Ciarlante, D. R., 1983: AC 252,214 — A new broad spectrum herbicide for soybeans: greenhouse studies. Proc. South Weed. Sci. Soc. 36, 90.
Ray, T. B., 1984: Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol. 75, 827–831.
Rogers, S. G., Brand, L. A., Holder, S. B., Sharps, E. S., Brackin, M. J., 1983: Amplification of the aroA gene from Escherichia coli results in tolerance to the herbicide glyphosate. Appl. Environ. Microbiol. 46, 37–43.
Rubin, J. L., Gaines, C., Jensen, R. A., 1984: Glyphosate inhibiton of 5-enolpyru-vylshikimate-3-phosphate synthase from suspension cultured cells of Nicotiana silvestris. Plant Physiol. 75, 839–846.
Schimke, R. T., 1984: Gene amplification in cultured animal cells. Cell 37, 705–713.
Schulz, A., Sost, D., Amrhein, N., 1984: Insensitivity of 5-enolpyruvylshikimic acid 3-phosphate synthase confers resistance to this herbicide in a strain of Aero-bacter aerogenes. Arch. Microbiol. 137, 121–123.
Shah, D. M., Horsch, R. B., Klee, H. J., Kishore, G. M., Winter, J. A., Tumer, N. E., Hironaka, C. M., Sanders, P. R., Gasser, C. S., Aykent, S., Siegel, N. R., Rogers, S. G., Fraley, R. T., 1986: Engineering herbicide tolerance in transgenic plants. Science 233, 478–481.
Shaner, D. L., Robson, P., Simcox, P. D., Ciarlante, D. R., 1983: Absorption, translocation and metabolism of AC 252,214 in soybeans, cocklebur and velvetleaf. Proc. South Weed. Sci. Soc. 36, 92.
Shaner, D. L., Anderson, P. C., Stidham, M. A., 1984: Imidazolinones-potent inhibitors of acetohydroxyacid synthase. Plant Physiol. 76, 545–546.
Siehl, D. L., Singh, B. K., Conn, E. E., 1986: Tissue distribution and subcellular localization of prephenate aminotransferase in leaves of Sorghum bicolor. Plant Physiol. 81, 711–713.
Singer, S. R., McDaniel, C. N., 1985: Selection of glyphosate-tolerant calli and the expression of this tolerance in regenerated plants. Plant Physiol. 78, 411–416.
Skokut, T. A., Wolk, C. P., Thomas, J., Meeks, J. C., Shaffer, P. W., 1987: Initial organic products of assimilation of [13N] ammonium and [13N] nitrate by tobacco cells cultured on different sources of nitrogen. Plant Physiol. 62, 299–304.
Smart, C., Johanning, D., Muller, G., Amrhein, N., 1985: Selective overproduction of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which tolerates high doses of the herbicide glyphosate. J. Biol. Chem. 260, 16338–16346.
Smith, C. M., Pratt, D., Thompson, G. A., 1985: Increased 5-enolpyruvylshikimic acid 3-phosphate synthase activity in a glyphosate-tolerant variant strain of tomato cells. Plant Cell Rep. 5, 298–301.
Sost, D., Schulz, A., Amrhein, N., 1984: Characterization of a glyphosate-insensitive 5-enolpyruvylshikimic acid-3-phosphate synthase. FEBS Lett. 238–242.
Stalker, D. M., Hiatt, W. R., Cornai, L., 1985: A single amino acid substitution of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate. J. Biol. Chem. 260, 4724–4728.
Steinrucken, H. C., Amrhein, N., 1980: The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem. Bio-phys. Res. Commun. 94, 1207–1212.
Steinrucken, H. C., Schulz, A., Amrhein, N., Porter, C. A., Fraley, R. T., 1986: Over-production of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant petunia hybrida cell line. Arch. Biochem. Biophys. 244, 169–173.
Sweetser, P. B., Schow, G. S., Hutchison, J. M., 1982: Metabolism of chlorsulfuron by plants: biological basis for selectivity of a new herbicide for cereals. Pestic. Biochem. Physiol. 17, 18–23.
Tachibana, K., Watanabe, T., Sekizuwa, Y., Takematsu, T., 1986: Action mechanism of bialaphos. 2. Accumulation of ammonia in plants treated with bi-alaphos. J. Pest. Sci. 11, 33–37.
Yadav, N., McDevitt, R. E., Benard, S., Falco, S. C., 1986: Single amino acid substitutions in the enzyme acetolactate synthase confer resistance to the herbicide sulfometuron methyl. Proc. Natl. Acad. Sci. 83, 4418–4422.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag/Wien
About this chapter
Cite this chapter
Shah, D.M., Gasser, C.S., della-Cioppa, G., Kishore, G.M. (1988). Genetic Engineering of Herbicide Resistance Genes. In: Verma, D.P.S., Goldberg, R.B. (eds) Temporal and Spatial Regulation of Plant Genes. Plant Gene Research. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6950-6_16
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6950-6_16
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-7448-7
Online ISBN: 978-3-7091-6950-6
eBook Packages: Springer Book Archive