Serology of potyviruses: current problems and some solutions

  • D. D. Shukla
  • R. Lauricella
  • C. W. Ward
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 5)


The serological relationships among members of the family Potyviridae are extremely complex and inconsistent. Variable cross-reactivity of polyclonal antisera, unexpected paired relationships between distinct viruses, and lack of cross-reactions between some strains are the major problems associated with the serology of potyviruses. Recent biochemical and immunochemical investigations of coat proteins have established the molecular basis for potyvirus serology and provided explanations for most of the problems with serology of potyviruses. Information from these studies has also formed the basis for the development of several novel approaches to the accurate detection and identification of potyviruses. However, even these novel approaches are not without drawbacks and some of them cannot be applied easily in plant virus laboratories, since they require prior sequence information and facilities for peptide synthesis. These findings suggest that serology is an imperfect criterion for the identification and classification of potyviruses.


Coat Protein Soybean Mosaic Virus Bean Common Mosaic Virus Bean Yellow Mosaic Virus Serological Relationship 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Allison RF, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9–20.PubMedCrossRefGoogle Scholar
  2. 2.
    Arnon R (1986) Synthetic peptides as the basis for future vaccines. Trends Biochem Sci 11: 521–524.CrossRefGoogle Scholar
  3. 3.
    Barnett OW, Randies JW, Burrows PM (1987) Relationships among Australian and North American isolates of the bean yellow mosaic potyvirus subgroup. Phytopathology 77: 791–799.CrossRefGoogle Scholar
  4. 4.
    Benjamin RJ, Waldman NH (1986) Induction of tolerance by monoclonal antibody therapy. Nature 320: 449–551.PubMedCrossRefGoogle Scholar
  5. 5.
    Bos L (1970) The identification of three new viruses isolated from Wisteria and Pisum in the Netherlands and the problem of variation within the potato virus Y group. Neth J Plant Pathol 76: 8–46.CrossRefGoogle Scholar
  6. 6.
    Cariepy J, Maitzner TM, Schoolnik GK (1986) Peptide antisera as sequence-specific probes of protein conformational transitions: calmodulin exhibits calcium dependent changes in antigenicity. Proc Natl Acad Sci USA 83: 8888–8892.CrossRefGoogle Scholar
  7. 7.
    Culvar JN, Sherwood JL (1988) Detection of peanut stripe virus in peanut seed by an indirect enzyme-linked immunosorbent assay using a monoclonal antibody. Plant Dis 72: 676–679.CrossRefGoogle Scholar
  8. 8.
    Diaco R, Hill JH, Durand DP (1986) Purification of soybean mosaic virus by affinity chromatography using monoclonal antibodies. J Gen Virol 67: 345–351.CrossRefGoogle Scholar
  9. 9.
    Dijkstra J, Bos L, Bouwmeester HJ, Hadiastono T, Lohuis H (1987) Identification of blackeye cowpea mosaic virus from germplasm of yard-long bean and from soybean, and the relationships between blackeye cowpea mosaic virus and cowpea aphid-borne mosaic virus. Neth J Plant Pathol 93: 115–133.CrossRefGoogle Scholar
  10. 10.
    Dougherty WG, Willis L, Johnston RF (1985) Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144: 66–73.PubMedCrossRefGoogle Scholar
  11. 11.
    Fortass M, Bos L, Goldbach RW (1991) Identification of potyvirus isolates from faba beans (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus. Arch Virol 118: 87–100.PubMedCrossRefGoogle Scholar
  12. 12.
    Francki RIB (1983) Current problems in plant virus taxonomy. In: Matthews REF (ed) A critical appraisal of viral taxonomy. CRC Press, Boca Raton, pp 63–104.Google Scholar
  13. 13.
    Francki RIB, Milne RG, Hatta T (1985) Atlas of plant viruses, vol 2. CRC Press, Boca Raton.Google Scholar
  14. 14.
    Frenkel MJ, Jilka JM, McKernNM, Strike PM, Clark Jr JM, Shukla DD, Ward CW (1991) Unexpected sequence diversity in the amino terminal ends of the coat proteins of strains of sugarcane mosaic virus. J Gen Virol 72: 237–242.PubMedCrossRefGoogle Scholar
  15. 15.
    Geysen HM, Mason TJ, Rodda SJ (1988) Cognitive features of continuous antigenic determinants. J Mol Recog 1: 32–41.CrossRefGoogle Scholar
  16. 16.
    Geysen HM, Meloen RH, Barteling SJ (1984) Use of peptide synthesis to probe viral antigens for epitopes to a resolution of single amino acids. Proc Natl Acad Sci USA 81: 3998–4002.PubMedCrossRefGoogle Scholar
  17. 17.
    Geysen HM, Rodda SJ, Mason TJ, Tribbick G, Schoofs PG (1987) Strategies for epitope analysis using peptide synthesis. J Immunol Methods 102: 259–274.PubMedCrossRefGoogle Scholar
  18. 18.
    Geysen HM, Shukla DD, Lauricella R, Plompen S, Tribbick G, Ward CW (1990) Synthetic peptides in the diagnosis of plant viruses. In: Abstracts of the VIIIth International Congress for Virology, Berlin, August 26–31, 1990, abstract W32–002.Google Scholar
  19. 19.
    Gibbs AJ (1977) Tobamovirus group. CMI/AAB Descriptions of Plant Viruses, no 184.Google Scholar
  20. 20.
    Gough KH, Shukla DD (1992) Major sequence variation in the N-terminal region of the capsid protein of a severe strain of passionfruit woodiness virus. Arch Virol 124: 389–396.PubMedCrossRefGoogle Scholar
  21. 21.
    Harrison BD (1985) Usefulness and limitations of the species concept for plant viruses. Intervirology 25: 71–78.CrossRefGoogle Scholar
  22. 22.
    Hill EK, Hill JH, Durand DP (1984) Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. J Gen Virol 65: 525–532.PubMedCrossRefGoogle Scholar
  23. 23.
    Himmler G, Brix U, Steinkellner H, Laimer M, Mattanovich D, Kattinger HWD (1988) Early screening of anti-plum pox virus monoclonal antibodies with different epitope specificities by means of gold-labelled immunosorbent electron microscopy. J Virol Methods 22: 351–358.PubMedCrossRefGoogle Scholar
  24. 24.
    Hollings M, Brunt AA (1981) Potyviruses. In: Kurstak E (ed) Handbook of plant virus infections: comparative diagnosis. Elsevier/North- Holland, Amsterdam, pp 731–807.Google Scholar
  25. 25.
    Hollings M, Brunt AA (1981) Potyvirus group. CMI/AAB Description of Plant Viruses, no 245.Google Scholar
  26. 26.
    Jordan R, Hammond J (1991) Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. J Gen Virol 72: 25–36.PubMedCrossRefGoogle Scholar
  27. 27.
    Koenig R, Bercks R (1968) Änderungen in heterologen Reaktionsvermögen von Antiseren gegen Vertreter der potato virus X-Gruppe im Laufe des Immunosierungs-prozesses. Phytopathol Z 61: 382–392.CrossRefGoogle Scholar
  28. 28.
    Koenig R, Lesemann DE (1979) Tymovirus group. CMI/AAB Description of Plant Viruses, no 214.Google Scholar
  29. 29.
    Lana AF, Lohuis H, Bos L, Dijkstra J (1988) Relationships among strains of bean common mosaic virus and blackeye cowpea mosaic virus-members of the potyvirus group. Ann Appl Biol 113: 493–505.CrossRefGoogle Scholar
  30. 30.
    Lauriceila R, Shukla DD, Plompen S, Morgan P, Stanton D, Harrison M, McKerral M (1990) Production of affinity purified poty virus-specific antisera using peptides shown to bind virus-specific antibodies. In: Abstracts of the Fifteenth Annual Lorne Conference on Protein Structure and Function, Lorne, Australia, February 11–15, 1990.Google Scholar
  31. 31.
    Moghal SM, Francki RIB (1976) Towards a system for the identification and classification of potyviruses. I. Serology and amino acid composition of six distinct viruses. Virology 73: 350–362.Google Scholar
  32. 32.
    Nelson MR, Wheeler RE (1978) Biological and serological characterization and separation of potyviruses that infect peppers. Phytopathology 68: 979–984.CrossRefGoogle Scholar
  33. 33.
    Purcifull DE, Zitter TA, Hiebert E (1975) Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65: 559–562.CrossRefGoogle Scholar
  34. 34.
    Scott SW, McLaughlin MR, Ainsworth AJ (1989) Monoclonal antibodies produced to bean yellow mosaic virus, clover yellow vein virus, and pea mosaic virus which cross-react among the three viruses. Arch Virol 108: 161–167.PubMedCrossRefGoogle Scholar
  35. 35.
    Shukla DD, Ward CW (1988) Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. J Gen Virol 69: 2703–2710.CrossRefGoogle Scholar
  36. 36.
    Shukla DD, Ward CW (1989) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Adv Virus Res 36: 273–314.PubMedCrossRefGoogle Scholar
  37. 37.
    Shukla DD, Ward CW (1989) Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Arch Virol 106: 171–200.PubMedCrossRefGoogle Scholar
  38. 38.
    Shukla DD, Frenkel MJ, McKern NM, Ward CW (1991) Immunological and molecular approaches to the diagnosis of viruses infecting horticultural crops. In: Prakash J, Peirik RLM (eds) Horticulture - new technologies and applications. Kluwer, Dordrecht, pp 311–319.CrossRefGoogle Scholar
  39. 39.
    Shukla DD, Frenkel MJ, McKern NM, Ward CW, Jilka J, Tosic M, Ford RE (1992) Present status of the sugarcane mosaic subgroup of potyviruses. In: Barnett OW (ed) Potyvirus taxonomy. Springer, Wien New York, pp 363–373 (Arch Virol [Suppl] 5).CrossRefGoogle Scholar
  40. 40.
    Shukla DD, Ford RE, Tosic M, Jilka J, Ward CW (1989) Possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. Arch Virol 105: 143–151.PubMedCrossRefGoogle Scholar
  41. 41.
    Shukla DD, Inglis AS, McKern NM, Gough KH (1986) Coat protein of potyviruses. 2. Amino acid sequence of coat protein of potato virus Y. Virology 152: 118–125.PubMedCrossRefGoogle Scholar
  42. 42.
    Shukla DD, Jilka J, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affintity purified polyclonal antibodies directed towards virus-specific N termini of coat proteins. J Gen Virol 70: 13–23.CrossRefGoogle Scholar
  43. 43.
    Shukla DD, McKern NM, Barnett OW, Ward CW (1990) Identification and classification of potyviruses infecting tropical legumes. In: Abstracts of the VIIIth International Congress of Virology, Berlin, August 26–31, 1991, abstract W87–008.Google Scholar
  44. 44.
    Shukla DD, McKernNM, Ward CW (1988) Coat protein of potyviruses. 5. Symptomatology, serology and coat protein amino acid sequences of three strains of passionfruit woodiness virus. Arch Virol 102: 221–232.PubMedCrossRefGoogle Scholar
  45. 45.
    Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N and C termini of the coat proteins of potyviruses are surface located and the N terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508.CrossRefGoogle Scholar
  46. 46.
    Shukla DD, Thomas JE, McKernNM, Tracy SL, Ward CW (1988) Coat protein of potyviruses. 4. Comparison of biological properties, serological relationships and coat protein amino acid sequence of four strains of potato virus Y. Arch Virol 102: 207–219.PubMedCrossRefGoogle Scholar
  47. 47.
    Shukla DD, Tosic M, Ford RE, Jilka J, Toler RW, Langham MAC (1989) Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79: 223–229.CrossRefGoogle Scholar
  48. 48.
    Shukla DD, Tribbick G, Mason TJ, Hewish DR, Geysen HM, Ward CW (1989) Localisation of virus-specific and group-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments. Proc Natl Acad Sci USA 86:8192–9196.PubMedCrossRefGoogle Scholar
  49. 49.
    Tracy SL, Frenkel MJ, Gough KH, Hanna PJ, Shukla DD (1992) Bean yellow mosaic, clover yellow vein and pea mosaic are distinct potyviruses: evidence from coat protein gene sequences and molecular hybridization involving 3-non-coding regions. Arch Virol 122: 249–261.PubMedCrossRefGoogle Scholar
  50. 50.
    Van Regenmortel MHV (1982) Serology and immunochemistry of plant viruses. Academic Press, New York.Google Scholar
  51. 51.
    Van Regenmortel MHV, von Wechmar B (1970) A reexamination of the serological relationship between tobacco mosaic virus and cucumber virus 4. Virology 41:330–338.PubMedCrossRefGoogle Scholar
  52. 52.
    Walkey DGA, Webb MJB (1984) The use of simple electron microscope serology procedure to observe relationships of seven potyviruses. Phytopathol Z 110: 319–327.CrossRefGoogle Scholar
  53. 53.
    Ward CW, Shukla DD (1991) Taxonomy of potyviruses: current problems and some solutions. Intervirology 32: 269–296.PubMedGoogle Scholar
  54. 54.
    Werkmeister JA, Shukla DD (1991) Selection of polyclonal antibodies to potyvirus-specific N terminus of coat proteins by induction of tolerance with monoclonal antibody. J Virol Methods 34: 71–79.PubMedCrossRefGoogle Scholar
  55. 55.
    Werkmeister JA, Tebb TA, Kirkpatrick A, Shukla DD (1991) The use of peptide-mediated electrofusion to select monoclonal antibodies against specific and homologous regions of the potyvirus coat protein. J Immunol Methods 143: 151–157.PubMedCrossRefGoogle Scholar
  56. 56.
    Wojchowski DM, Sytkowski AJ (1986) Hybridoma production by simplified avidin-mediated electrofusion. J Immunol Methods 90: 173–177.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. D. Shukla
    • 1
    • 3
  • R. Lauricella
    • 2
  • C. W. Ward
    • 1
  1. 1.Division of Biomolecular EngineeringCSIROParkvilleAustralia
  2. 2.Chiron Mimotopes Pty Ltd.ClaytonAustralia
  3. 3.Division of Biomolecular EngineeringCSIROParkvilleAustralia

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