Fungal transmission of a potyvirus: uredospores of Puccinia sorghi transmit maize dwarf mosaic virus
Maize dwarf mosaic virus (MDMV) and maize rust, Puccinia sorghi Schw., occur as natural infections on cultivated maize in South Africa. P. sorghi often occurs as a secondary late infection on maize plants which have already been infected with MDMV earlier in the season, either seed or aphid transmitted. When MDMV isolates from maize plants naturally infected by both virus and fungus were propagated by sap inoculation in plant growth rooms, residual uredospores in the sap gave rise to the development of uredia under conditions of high humidity. When uredospores developing on MDMV-B-infected plants were germinated on virus free maize seedlings, these plants became infected with MDMV-B. Similarly, when uredospores, originating from maize plants infected with MDMV-A, were scattered onto virus free maize seedlings, these plants became infected with MDMV-A.
The presence of virus on uredospores in infected plant tissue was visualized by indirect immunofluorescence. Identification of virus infection was by DAS-ELISA and immunoelectro—blotting utilizing strain—specific antisera. Virus transmission occurred between closely situated plants which had no actual contact (unaided transmission). MDMV-B transmission by uredospores, to new maize seedlings, has been maintained for three successive years (1988–1991) in a plant growth room. The MDMV-B isolate remained sap and non—persistently aphid transmissible.
KeywordsMaize Seedling Leaf Section Commercial Hybrid Rust Infection Maize Dwarf Mosaic Virus
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- 3.Chauhan R (1985) A study of filamentous viruses in maize and small grains. MSc Thesis, University of Cape Town, Cape Town.Google Scholar
- 5.Erasmus DS (1982) The association of bromegrass mosaic virus (BMV) with Puccinia graminis tritici. MSc Thesis, University of Cape Town, Cape Town.Google Scholar
- 13.Rybicki EP, von Wechmar MB (1985) Serology and immunochemistry. In: Francki RIB (ed) The plant viruses, vol I. Plenum, New York, pp 207–244.Google Scholar
- 14.Shurtleff MC (ed) (1980) Compendium of corn diseases, 2nd edn. American Phytopathological Society, St. Paul.Google Scholar
- 16.von Wechmar MB, Chauhan R, Knox E (1984) Seed transmitted viruses in maize. In: Proceedings of the 6th South African Maize Breeding Symposium. Department of Agriculture, Technical Communication, pp 52–55.Google Scholar
- 17.von Wechmar MB, Chauhan R, Knox E (1988) Application of immunoelectroblotting to differentiate between isolates of maize dwarf virus and sugarcane mosaic virus. In: Proceedings of the 8th South African Maize Breeding Symposium. Department of Agriculture, Technical Communication, pp 41–44.Google Scholar
- 18.von Wechmar MB, Knox E (1988) Distribution of maize dwarf mosaic virus strains A and B in South African maize, sweet corn, sorghum, several grasses and sugarcane. In: Proceedings of the 8th South African Maize Breeding Symposium. Department of Agriculture, Technical Communication, pp 44–48.Google Scholar
- 19.Walker FD, Batty I, Thomson RD (1971) The localization of bacterial antigens by the use of fluorescent and ferritin labelled antibody techniques. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol V. American Society of Microbiology, Washington, DC, pp 119–247.Google Scholar