Potyvirus serology, sequences and biology

  • J. Hammond
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 5)


Amino acid sequences of the cytoplasmic cylindrical inclusion protein (CIP), large nuclear inclusion protein (NIb), and coat protein (CP) of potyviruses were re-examined in light of reported serological relationships, and correlated with known and deduced biological functions. No obvious correlations were observed between either amino acid sequences or epitopes recognized by monoclonal antibodies and the natural host ranges of the potyviruses examined. Whereas the identified sequence motifs of the RNA helicase (CIP) and replicase (NIb) are predicted to be antigenic, most of the conserved sequences and epitopes in the CIP, NIb and CP were presumed to be maintained for structural rather than functional reasons. Three possible potyvirus clusters are proposed on the basis of the length and composition of the virion surface-exposed amino terminal extension of the CP; these clusters do not correlate with overall CP sequence homology, host range, or vectors, but are of potential evolutionary significance and hence of possible taxonomie value.


Coat Protein Potato Virus Tobacco Etch Virus Soybean Mosaic Virus Zucchini Yellow Mosaic Virus 
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  1. 1.
    Allison R, Johnston RE, Dougherty WG (1986) The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9–20.PubMedCrossRefGoogle Scholar
  2. 2.
    Allison RF, Sorenson JC, Kelly ME, Armstrong FB, Dougherty WG (1985) Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for synthesis and processing of a polyprotein in potyvirus gene expression. Proc Natl Acad Sci USA 82: 3969–3972.PubMedCrossRefGoogle Scholar
  3. 3.
    Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly M, Armstrong FB (1985) Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147: 309–316.PubMedCrossRefGoogle Scholar
  4. 4.
    Atreya CD, Raccah B, Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178: 161–165.PubMedCrossRefGoogle Scholar
  5. 5.
    Boye K, Jensen PE, Stummann BM, Henningsen KW (1990) Nucleotide sequence of cDNA encoding the BYMV coat protein gene. Nucleic Acids Res 18: 4926.PubMedCrossRefGoogle Scholar
  6. 6.
    Bravo-Almontacid F, Mentaberry AN (1989) Nucleotide cDNA sequence coding for the PVY0 coat protein. Nucleic Acids Res 17: 4401.CrossRefGoogle Scholar
  7. 7.
    Burger JT, Brand RJ, Rybicki EP (1990) The molecular cloning and nucleotide sequencing of the ′ terminal region of Ornithogalum mosaic virus. J Gen Virol 71: 2527–2534.PubMedCrossRefGoogle Scholar
  8. 8.
    Domier LL, Franklin KM, Shahabuddin M, Hellmann GM, Overmeyer JH, Hiremath ST, Siaw MFE, Lomonossoff GP, Shaw JG, Rhoads RE (1986) The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Res 14: 5417–5430.PubMedCrossRefGoogle Scholar
  9. 9.
    Dougherty WG, Allison RF, Parks TD, Johnston RE, Feild MJ, Armstrong FB (1985) Nucleotide sequence at the ′ terminus of pepper mottle virus genomic RNA: evidence for an alternate mode of potyvirus capsid protein gene organization. Virology 146: 282–291.PubMedCrossRefGoogle Scholar
  10. 10.
    Dougherty WG, Willis L, Johnston RE (1985) Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144: 66–72.PubMedCrossRefGoogle Scholar
  11. 11.
    Eggenberger AL, Stark DM, Beachy RN (1989) The nucleotide sequence of a soybean mosaic virus coat protein-coding region and its expression in Escherichia coli, Agrobacterium tumefaciens and tobacco callus. J Gen Virol 70: 1853–1860.PubMedCrossRefGoogle Scholar
  12. 12.
    Gal-On A, Antignus Y, Rosner A, Raccah B (1990) Nucleotide sequence of the zucchini yellow mosaic virus capsid-encoding gene and its expression in Escherichia coli. Gene 87: 273–277.PubMedCrossRefGoogle Scholar
  13. 13.
    Gough KH, Azad AA, Hanna D, Shukla DD (1987) Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnsongrass strain of sugarcane mosaic virus RNA. J Gen Virol 68: 297–304.CrossRefGoogle Scholar
  14. 14.
    Govier DA, Kassanis B, Pirone TP (1977) Partial purification and characterization of the potato virus Y helper component. Virology 78: 306–314.PubMedCrossRefGoogle Scholar
  15. 15.
    Grumet R, Fang G (1990) cDNA cloning and sequence analysis of the ′ terminal region of zucchini yellow mosaic virus RNA. J Gen Virol 71: 1619–1622.PubMedCrossRefGoogle Scholar
  16. 16.
    Gugerli P, Fries P (1983) Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J Gen Virol 64: 2471–2477.CrossRefGoogle Scholar
  17. 17.
    Hammond J (1989) Antisera to cytoplasmic inclusion proteins of potyviruses contain cross-reactive antibodies. Phytopathology 79: 1174.Google Scholar
  18. 18.
    Hammond J, Hammond RW (1989) Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. J Gen Virol 70: 1961–1974.PubMedCrossRefGoogle Scholar
  19. 19.
    Hammond J, Lawson RH, Hsu HT (1985) Use of a monoclonal antibody reactive with several potyviruses for detection and identification in combination with virus-specific antisera. Phytopathology 75: 1353.Google Scholar
  20. 20.
    Hammond J, Jordan RL, Kamo KK (1990) Use of chimeric coat protein constructs and deletion mutants to examine potyvirus structure and coat protein-mediated resistance. Phytopathology 80: 1018.Google Scholar
  21. 21.
    Hellmann GM, Thornbury DW, Pirone TP (1990) Molecular analysis of tobacco vein mottling virus (TVMV) pathogenicity by infectious transcripts of chimeric potyviral cDNA genomes. Phytopathology 80: 1036.Google Scholar
  22. 22.
    Hiebert E, Tremaine JH, Ronald WP (1984) The effect of limited proteolysis on the amino acid composition of five potyviruses and on the serological reaction and peptide map of the tobacco etch virus capsid protein. Phytopathology 74: 411–416.CrossRefGoogle Scholar
  23. 23.
    Hill EK, Hill JH, Durand DP (1984) Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. J Gen Virol 65: 525–532.PubMedCrossRefGoogle Scholar
  24. 24.
    Hopp TP, Woods KR (1981) Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci USA 78: 3824–3828.PubMedCrossRefGoogle Scholar
  25. 25.
    Hsu HT, Franssen JM, Hammond J, Derks AFLM, Lawson RH (1986) Some properties of mouse monoclonal antibodies produced to tulip breaking virus. Phytopathology 76: 1132.Google Scholar
  26. 26.
    Hsu HT, Franssen JM, Van Der Hulst CTC, Derks AFLM, Lawson RH (1988) Factors affecting selection of epitope specificity of monoclonal antibodies to tulip breaking potyvirus. Phytopathology 78: 1337–1340.CrossRefGoogle Scholar
  27. 27.
    Jayaram C, Hill JH, Miller WA (1991) Nucleotide sequences of the coat protein genes of two aphid-transmissible strains of soybean mosaic virus. J Gen Virol 72: 1001–1003.PubMedCrossRefGoogle Scholar
  28. 28.
    Jensen SG, Staudinger JL (1989) Serological grouping of the cytoplasmic inclusions of 6 strains of sugarcane mosaic virus. Phytopathology 79: 1215.Google Scholar
  29. 29.
    Jilka JM (1990) Cloning and characterization of the ′ terminal regions of RNA from select strains of maize dwarf mosaic virus and sugar cane mosaic virus. PhD dissertation, University of Illinois at Urbana-Champaign.Google Scholar
  30. 30.
    Jordan RL (1989) Mapping of potyvirus-specific and group-common antigenic determinants with monoclonal antibodies by Western-blot analysis and coat protein amino acid sequence comparisons. Phytopathology 79: 1157.CrossRefGoogle Scholar
  31. 31.
    Jordan RL, Hammond J (1986) Analysis of antigenic specificity of monoclonal antibodies to several potyviruses. Phytopathology 76: 1091.Google Scholar
  32. 32.
    Jordan RL, Hammond J (1991) Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. J Gen Virol 72: 25–36.PubMedCrossRefGoogle Scholar
  33. 33.
    Karplus PA, Schulz GE (1985) Prediction of chain flexibility in proteins. Naturwissenschaften 72: 212–213.CrossRefGoogle Scholar
  34. 34.
    Lain S, Riechmann JL, Mendez E, Garcia JA (1988) Nucleotide sequence of the ′ terminal region of plum pox potyvirus RNA. Virus Res 10: 325–342.CrossRefGoogle Scholar
  35. 35.
    Lain S, Riechmann JL, Garcia JA (1990) RNA helicase: a novel activity associated with a protein encoded by a positive stranded RNA virus. Nucleic Acids Res 18: 7003–7006.PubMedCrossRefGoogle Scholar
  36. 36.
    Maiss E, Timpe U, Brisske A, Jelkmann W, Casper R, Himmler G, Mastanovich D, Katinger HWD (1989) The complete nucleotide sequence of plum pox virus RNA. J Gen Virol 70:513–524.PubMedCrossRefGoogle Scholar
  37. 37.
    Nagel J, Hiebert E (1985) Complementary DNA cloning and expression of the papaya ringspot potyvirus sequences encoding capsid protein and a nuclear inclusion-like protein in Escherichia coli. Virology 143: 435–441.PubMedCrossRefGoogle Scholar
  38. 38.
    Quemada H, L’Hostis B, Gonsalves D, Reardon IM, Heinrikson R, Hiebert EL, Sieu LC, Slightom JL (1990) The nucleotide sequence of the ′ terminal regions of papaya ringspot virus strains W and P. J Gen Virol 71: 203–210.PubMedCrossRefGoogle Scholar
  39. 39.
    Quemada H, Sieu LC, Siemeniak DR, Gonsalves D, Slightom JL (1990) Watermelon mosaic virus II and zucchini yellow mosaic virus: cloning of ′ terminal regions, nucleotide sequences, and phylogenetic comparisons. J Gen Virol 71: 1451–1460.PubMedCrossRefGoogle Scholar
  40. 40.
    Raccah B, Pirone TP (1984) Characteristics of and factors affecting helper component-mediated aphid transmission of a potyvirus. Phytopathology 74: 305–308.CrossRefGoogle Scholar
  41. 41.
    Ravelonandro M, Varveri C, Delbos R, Dunez J (1988) Nucleotide sequence of the capsid protein gene of plum pox potyvirus. J Gen Virol 69: 1509–1516.CrossRefGoogle Scholar
  42. 42.
    Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F (1989) Nucleotide sequence of potato virus Y (N strain) genomic RNA. J Gen Virol 70: 935–947.PubMedCrossRefGoogle Scholar
  43. 43.
    Rosner A, Raccah B (1988) Nucleotide sequence of the capsid protein gene of potato virus Y (PVY). Virus Genes 1: 255–260.PubMedCrossRefGoogle Scholar
  44. 44.
    Salomon R (1989) Partial cleavage of sweet potato feathery mottle virus coat protein sub-unit by an enzyme in extracts of infected symptomless leaves. J Gen Virol 70:1943–1949.PubMedCrossRefGoogle Scholar
  45. 45.
    Scott SW, McLaughlin MR, Ainsworth AJ (1989) Monoclonal antibodies produced to bean yellow mosaic virus, clover yellow vein virus, and pea mosaic virus which cross-react among the three viruses. Arch Virol 108: 161–167.PubMedCrossRefGoogle Scholar
  46. 46.
    Shukla DD, Ward CW (1989) Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Arch Virol 102: 171–200.CrossRefGoogle Scholar
  47. 47.
    Shukla DD, Inglis AS, McKern NM, Gough KH (1986) Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152: 118–125.PubMedCrossRefGoogle Scholar
  48. 48.
    Shukla DD, McKern NM, Ward CW (1988) Coat protein of potyviruses. 5. Symptomatology, serology and coat protein sequences of three strains of passionfruit woodiness virus. Arch Virol 102: 221–232.PubMedCrossRefGoogle Scholar
  49. 49.
    Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N and C termini of the coat proteins of potyviruses are surface-located and the N-terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508.CrossRefGoogle Scholar
  50. 50.
    Shukla DD, Tosic M, Jilka J, Ford RE, Toler RW, Langham MAC (1989) Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79: 223–229.CrossRefGoogle Scholar
  51. 51.
    Shukla DD, Jilka J, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affinity-purified polyclonal antibodies directed towards virus-specific N-termini of coat proteins. J Gen Virol 70: 13–22.CrossRefGoogle Scholar
  52. 52.
    Takahashi T, Uyeda I, Ohshima K, Shikata E (1990) Nucleotide sequence of the capsid protein gene of bean yellow mosaic virus chlorotic spot strain. J Fac Agric Hokkaido Univ 64: 152–163.Google Scholar
  53. 53.
    Thornbury DW, Patterson CA, Dessens JT, Pirone TP (1990) Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C. Virology 178: 573–578.PubMedCrossRefGoogle Scholar
  54. 54.
    Timmerman GM, Calder VL, Bolger LEA (1990) Nucleotide sequence of the coat protein gene of pea seedborne mosaic potyvirus. J Gen Virol 71: 1869–1872.PubMedCrossRefGoogle Scholar
  55. 55.
    Tremblay MF, Nicolas O, Sinha RC, Lazure C, Laliberte JF (1990) Sequence of the ′ terminal region of turnip mosaic virus RNA and the capsid protein gene. J Gen Virol 71:2769–2772.PubMedCrossRefGoogle Scholar
  56. 56.
    Turpen T (1989) Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. J Gen Virol 70: 1951–1960.PubMedCrossRefGoogle Scholar
  57. 57.
    Uyeda I, Takahashi T, Shikata E (1991) Relatedness of nucleotide sequence of the ′ terminal region of clover yellow vein potyvirus to bean yellow mosaic potyvirus RNA. Intervirology 32: 234–245.PubMedGoogle Scholar
  58. 58.
    Van Regenmortel MHV, von Wechmar MB (1970) A re-examination of the serological relationship between tobacco mosaic virus and cucumber virus 4. Virology 41: 330–338.PubMedCrossRefGoogle Scholar
  59. 59.
    Yu M, Frenckel MJ, McKern NM, Shukla DD, Strike PM, Ward CW (1989) Coat protein of the potyviruses. 6. Amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus are strains of the same potyvirus. Arch Virol 105: 55–64.PubMedCrossRefGoogle Scholar
  60. 60.
    Zitter TA (1972) Naturally occurring pepper virus strains in Florida. Plant Dis Rep 56: 586–590.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Hammond
    • 1
    • 2
  1. 1.Florist and Nursery Crops Laboratory, Beltsville Agricultural Research CenterUnited States Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.FNCLUSDA-ARSBeltsvilleUSA

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