Potyviruses, monoclonal antibodies, and antigenic sites

  • R. Jordan
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 5)


Virus-specific and cross-reactive monoclonal antibodies have been produced to at least 19 different aphid-transmitted potyviruses. This report summarizes the development of these monoclonal antibodies as well as presents information on the delineation of the virus-specific and group-common epitopes defined by these monoclonal antibodies. Virus-specific and group-common antigenic determinants were mapped by a variety of techniques, including analysis of antigen: antibody reactivity patterns, determination of N-terminal vs. trypsin-resistant core peptide-specificity, immuno-analysis of overlapping synthetic peptides, and immunoanalysis of bacterially expressed coat-protein gene products. Of those monoclonal antibodies that have been examined, monoclonal antibody-defined virus-specific epitopes are virion surface-located within the 30+ amino acid amino terminus, whereas the group-common epitopes are found in the trypsin-resistant core protein not usually located on the virion surface, as has been shown previously with certain polyclonal antibodies. New information is presented on the analysis of bean yellow mosaic virus amino terminal epitopes as well as on the identification of amino terminal antigenic determinants shared between strains of bean yellow mosaic virus and pepper mottle virus. A recommendation on the evaluation and use of a panel of potyvirus broad-spectrum reacting monoclonal as reference monoclonal antibodies for the detection and classification of aphid-transmitted potyviruses is also presented.


Tobacco Etch Virus Zucchini Yellow Mosaic Virus Bean Common Mosaic Virus Maize Dwarf Mosaic Virus Bean Yellow Mosaic Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly M, Armstrong FB (1985) Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147: 309–316.PubMedCrossRefGoogle Scholar
  2. 2.
    Baker CA, Lecoq H, Purcifull DE (1991) Serological and biological variability among papaya ringspot virus type-W isolates in Florida. Phytopathology 81: 722–728.CrossRefGoogle Scholar
  3. 3.
    Baker CA, Purcifull DE (1988) Reactivity of a monoclonal antibody to the amorphous inclusion protein of papaya ringspot virus type-W (PRSV-W). Phytopathology 78: 1537.Google Scholar
  4. 4.
    Baker CA, Purcifull DE (1990) Reactivity of two monoclonal antibodies to the cylindrical inclusion protein of papaya ringspot virus type-W. Phytopathology 80: 1033.Google Scholar
  5. 5.
    Barnett OW, Randies JW, Burrows PM (1987) Relationships among Australian and North American isolates of the bean yellow mosaic potyvirus subgroup. Phytopathology 77: 791–799.CrossRefGoogle Scholar
  6. 6.
    Boonekamp PM, Pomp H (1986) Problems concerning the production of monoclonal antibodies for plant diagnostic purposes. Acta Hortic 177: 103–109.Google Scholar
  7. 7.
    Boye K, Jensen PE, Stummann BM, Henningsen KW (1990) Nucleotide sequence of cDNA encoding the BYMV coat-protein gene. Nucleic Acids Res 18:4926.PubMedCrossRefGoogle Scholar
  8. 8.
    Culver JN, Sherwood JL (1988) Detection of peanut stripe virus in peanut seed by an indirect enzyme-linked assay using a monoclonal antibody. Phytopathology 72: 676–679.Google Scholar
  9. 9.
    Dougherty WG, Allison RF, Parks TD, Johnston RE, Feild MJ, Armstrong FB (1985) Nucleotide sequence at the 3′-terminus of the pepper mottle virus genomic RNA: evidence for an alternative mode of capsid protein gene organization. Virology 146: 282–291.PubMedCrossRefGoogle Scholar
  10. 10.
    Dougherty WG, Parks TD (1991) Post-translational processing of the tobacco etch virus 49 small nuclear inclusion polyprotein: identification of an internal cleavage site and delimitation of VPg and proteinase domains. Virology 183: 449–456.PubMedCrossRefGoogle Scholar
  11. 11.
    Dougherty WG, Willis L, Johnston RE (1985) Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144: 66–72.PubMedCrossRefGoogle Scholar
  12. 12.
    Fernandez-Northcote EN, Gugerli P (1985) Reaction of a broad spectrum of PVY isolates to monoclonal antibodies in ELISA. Phytopathology 75: 1353.Google Scholar
  13. 13.
    Fortass M, Bos L, Goldbach RW (1991) Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus. Arch Virol 118: 87–100.PubMedCrossRefGoogle Scholar
  14. 14.
    Geysen HM, Mason TJ, Rodda SJ (1988) Cognitive features of continuous antigenic determinants. J Mol Recogn 1: 23–41.CrossRefGoogle Scholar
  15. 15.
    Geysen HM, Rodda SJ, Mason TJ, Tribbick G, Schoofs PG (1987) Strategies for epitope analysis using peptide synthesis. J Immunol Methods 102: 259–274.PubMedCrossRefGoogle Scholar
  16. 16.
    Gugerli P, Fries P (1983) Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J Gen Virol 64: 2471–2477.CrossRefGoogle Scholar
  17. 17.
    Hammond J (1992) Potyvirus serology, sequences and biology. In: Barnett OW (ed) Potyvirus taxonomy. Springer, Wien New York, pp 123–138 (Arch Virol [Suppl] 5).CrossRefGoogle Scholar
  18. 18.
    Hammond J, Hammond RW (1989) Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat-protein gene. J Gen Virol 70: 1961–1974.PubMedCrossRefGoogle Scholar
  19. 19.
    Hammond J, Jordan RL, Kamo KK (1990) Use of chimeric coat-protein constructs and deletion mutants to examine potyvirus structure and coat-protein-mediated resistance. Phytopathology 80: 1018.Google Scholar
  20. 20.
    Hammond J, Jordan RL, Larsen RC, Moyer JW (1992) Serological relationships between three filamentous viruses of sweet potato examined using polyclonal antisera and monoclonal antibodies. Phytopathology 82: 713–717.CrossRefGoogle Scholar
  21. 21.
    Hammond J, Lawson RH, Hsu HT (1985) Use of a monoclonal antibody reactive with several potyviruses for detection and identification in combination with virus-specific antisera. Phytopathology 75: 1353.Google Scholar
  22. 22.
    Hampton RO, Shukla, DD, Jordan RL (1992) White lupin mosaic virus: comparative host range, serology and coat-protein peptide profiles. Phytopathology 82: 566–571.CrossRefGoogle Scholar
  23. 23.
    Hill EK, Hill JH, Durand DP (1984) Production of monoclonal antibodies to viruses in the potyvirus group: use in radioimmunoassay. J Gen Virol 65: 525–532.PubMedCrossRefGoogle Scholar
  24. 24.
    HimmlerG, Brix U, SteinkellnerH, LaimerM, MattanovichD, KatingerHWD (1988) Early screening for anti-plum pox monoclonal antibodies with different specificities by means of gold-labelled immunosorbent electron microscopy. J Virol Methods 22: 351–358.PubMedCrossRefGoogle Scholar
  25. 25.
    Hsu HT, Franssen JM, Van der Hulst CTC, Derks AFLM, Lawson RH (1988) Factors affecting selection of epitope specificity of monoclonal antibodies to tulip breaking potyvirus. Phytopathology 78: 1337–1340.CrossRefGoogle Scholar
  26. 26.
    Jones FE, Hill JH, Durand DP (1988) Detection and differentiation of maize dwarf mosaic virus, strains of A and B, by use of different class immunoglobulins in a double-antibody sandwich enzyme-linked immunosorbent assay. Phytopathology 78:1118–1124.CrossRefGoogle Scholar
  27. 27.
    Jordan RL (1989) Mapping of potyvirus-specific and group-common antigenic determinants with monoclonal antibodies by Western-blot analysis and coat-protein amino acid sequence comparisons. Phytopathology 79: 1157.CrossRefGoogle Scholar
  28. 28.
    Jordan RL (1992) Mapping of bean yellow mosaic virus-specific and potyvirus group-common coat-protein antigenic determinants defined by monoclonal antibodies. Phytopathology (submitted).Google Scholar
  29. 29.
    Jordan RL, Hammond, J (1986) Analysis of antigenic specificity of monoclonal antibodies to several potyviruses. Phytopathology 76: 1091.Google Scholar
  30. 30.
    Jordan RL, Hammond J (1991) Comparison and differentiation of potyvirus isolates and identification of strain-, virus-, subgroup-specific and potyvirus group-common epitopes using monoclonal antibodies. J Gen Virol 72: 25–36.PubMedCrossRefGoogle Scholar
  31. 31.
    Jordan RL, Thornbury DW, Pirone TP (1990) Production and initial characterization of monoclonal antibodies to helper components of potato virus Y and tobacco vein mottling virus. Phytopathology 80: 1018.Google Scholar
  32. 32.
    Langeveld SA, Dore JM, Memlelink J, Derks AFLM, van der Vlugt CIM, Asjes CJ, Bol JF (1991) Identification of potyviruses using the polymerase chain reaction with degenerate primers. J Gen Virol 72: 1531–1541.PubMedCrossRefGoogle Scholar
  33. 33.
    Lenstra JA, Kusters JG, Van der Zeijst BAM (1990) Mapping of viral epitopes with prokaryotic expression products. Arch Virol 110: 1–24.PubMedCrossRefGoogle Scholar
  34. 34.
    Li RH, Zettler FW, Elliot MS, Petersen MA, Still PE, Baker CA, Mink GI (1991) A strain of peanut mottle virus seedborne in bambarra groundnut. Plant Dis 74: 130–133.CrossRefGoogle Scholar
  35. 35.
    Miller AW, Surgeoner R, Mills PR (1988) Production and characterization of monoclonal antibodies to bean common mosaic virus. Phytopathology 78: 1585.Google Scholar
  36. 36.
    Scott SW, McLaughlin MR, Ainsworth AJ (1989) Monoclonal antibodies produced to bean yellow mosaic virus, clover yellow vein virus, and pea mosaic virus which cross-react among the three viruses. Arch Virol 108: 161–167.PubMedCrossRefGoogle Scholar
  37. 37.
    Sherwood JL, Sanborn MR, Keyser GC (1987) Production of monoclonal antibodies to peanut mottle virus and their use in enzyme-linked immunosorbent assay and dot-blot immunobinding assay. Phytopathology 77: 1158–1161.CrossRefGoogle Scholar
  38. 38.
    Shukla DD, Ford RE, Tosic M, Jilka J, Ward CW (1989) Possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. Arch Virol 105: 143–151.PubMedCrossRefGoogle Scholar
  39. 39.
    Shukla DD, Frenkel MJ, Ward CW (1991) Structure and function of the potyvirus genome with special reference to the coat-protein coding region. Can J Plant Pathol 13: 178–191.CrossRefGoogle Scholar
  40. 40.
    Shukla DD, Jikla J, Tosic M, Ford RE (1989) A novel approach to the serology of potyviruses involving affinity-purified polyclonal antibodies directed towards virus-specific N-termini of coat-proteins. J Gen Virol 70: 13–22.CrossRefGoogle Scholar
  41. 41.
    Shukla DD, McKern NM, Gough KH, Tracy SL, Letho SG (1988) Differentiation of potyviruses and their strains by high performance liquid chromatographic peptide profiling of coat-proteins. J Gen Virol 69: 493–502.CrossRefGoogle Scholar
  42. 42.
    Shukla DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) The N and C termini of the coat-proteins of potyviruses are surface-located and the N-terminus contains the major virus-specific epitopes. J Gen Virol 69: 1497–1508.CrossRefGoogle Scholar
  43. 43.
    Shukla DD, Tribbick G, Mason TJ, Hewish DR, Geysen HM, Ward CW (1989) Localization of virus-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments. Proc Natl Acad Sci USA 86: 8192–8196.PubMedCrossRefGoogle Scholar
  44. 44.
    Shukla DD, Ward CW (1989) Structure of potyvirus coat-proteins and applications in the taxonomy of the potyvirus group. Adv Virus Res 36: 273–314.PubMedCrossRefGoogle Scholar
  45. 45.
    Shukla DD, Ward CW (1989) Identification and classification of potyviruses on the basis of coat-protein sequence data and serology. Arch Virol 106: 171–200.PubMedCrossRefGoogle Scholar
  46. 46.
    Slade DE, Johnston RE, Dougherty WG (1989) Generation and characterization of monoclonal antibodies reactive with the 49 proteinase of tobacco etch virus. Virology 173: 499–508.PubMedCrossRefGoogle Scholar
  47. 47.
    Takahashi T, Uyeda I, Ohshima K, Shikata E (1990) Nucleotide sequence of the capsid protein gene of bean yellow mosaic virus chlorotic spot strain. J Fac Agric Hokkaido Univ 64: 152–163.Google Scholar
  48. 48.
    Turpen T (1989) Molecular cloning of a potato virus Y genome: nucleotide sequence homology in non-coding regions of potyviruses. J Gen Virol 70: 1951–1960.PubMedCrossRefGoogle Scholar
  49. 49.
    Uyeda I, Takahashi T, Shikata E (1991) Relatedness of nucleotide sequence of the 3′ terminal region of clover yellow vein potyvirus to bean yellow mosaic potyvirus RNA. Intervirology 32: 234–245.PubMedGoogle Scholar
  50. 50.
    Vance VB, Jordan RL, Edwardson JR, Christie RG, Purcifull DE, Turpen T, Falk B (1992) Evidence that pepper mottle virus and potato virus Y are distinct viruses: analysis of the coat-protein and 3′ untranslated sequences of a California isolate of pepper mottle virus. In: Barnett OW (ed) Potyvirus taxonomy. Springer, Wien New York, pp 337–345 (Arch Virol [Suppl] 5).CrossRefGoogle Scholar
  51. 51.
    Wang W, Mink GI, Silbernagel MJ (1985) A broad spectrum monoclonal antibody prepared against bean common mosaic virus. Phytopathology 75: 1352.Google Scholar
  52. 52.
    Ward CW, Shukla DD (1991) Taxonomy of potyviruses: current problems and some solutions. Intervirology 32: 269–296.PubMedGoogle Scholar
  53. 53.
    Wisler GC, Baker CA, Purcifull DE, Hiebert E (1989) Partial characterization of monoclonal antibodies to zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus (WMV-2). Phytopathology 79: 1213.Google Scholar
  54. 54.
    Yao SK, Cai SH, Jia SR, Hsu HT (1985) Specificity of monoclonal antibodies to strains of potato virus Y. Phytopathology 75: 1356.Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • R. Jordan
    • 1
    • 2
  1. 1.Plant Sciences Institute, Florist and Nursery Crops LaboratoryUnited States Department of Agriculture, Agricultural Research ServiceBeltsvilleUSA
  2. 2.BARC-West, FNCLUSDA-ARSBeltsvilleUSA

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