Recent Advances in the Understanding of Membrane Opioid Receptor

  • A. P. Smith
  • H. H. Loh
Conference paper


The cloning of the ∂ opioid receptor by two independent groups has been quickly followed by cloning of the other two major opioid receptor types, µ and k. It is likely that other opioid receptor types of subtypes will soon be discovered, and the availability of the receptor cDNAs will also allow investigators to explore their localization and function. Our own laboratory, following a more traditional approach, has isolated a protein that seems to play some role in opioid receptor function.


Opioid Receptor Opiate Receptor Receptor cDNA Dorsal Root Ganglion Culture Opioid Ligand 
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  1. 1.
    Pert C B, Snyder S H. Opiate receptor: its demonstration in nervous tissue. Science 179: 1011–1014, 1973.Google Scholar
  2. 2.
    Simon E J, Hiller J M, Edelman J. Stereospecific binding of the potent narcotic analgesic (3H)etorphine to rat brain homogenate. Proc Natl Acad Sci USA 70: 1947–1949, 1973.PubMedCrossRefGoogle Scholar
  3. 3.
    Terenius L. Stereospecific interaction between narcotic analgesics and a synaptic plasma membrane fraction of rat cerebral cortex. Act Pharmacol 32: 317–320, 1973.CrossRefGoogle Scholar
  4. 4.
    Loh H H, Smith A P. Molecular characterization of opioid receptors. Ann Rev Pharmacol 30: 123–147, 1990.CrossRefGoogle Scholar
  5. 5.
    Evans C J, Kleith D E,Jr., Morrison K, Edwards R H. Cloning of a delta opioid receptor by functional expression. Science 258: 1952–1955, 1992.PubMedCrossRefGoogle Scholar
  6. 6.
    Kieffer B L, Befort K, Gaveriaux-Ruff C, Hirth C G. The a-opioid receptor: Isolation of a cDNA by expression cloning and pharmacological characterization. Proc Natl Acad Sci USA 89: 12048–12052, 1992.PubMedCrossRefGoogle Scholar
  7. 7.
    Koski G, Klee W A. Opiates inhibit adenylate cyclase by stimulating GTP hydrolysis. Proc Natl Acad Sci 78: 4185–4189, 1981.PubMedCrossRefGoogle Scholar
  8. 8.
    Childers S R, Snyder S H. Guanine nucleotides differentiate agonist and antagonist interactions with opiate receptors. Life Sci 33: 759–762, 1978.CrossRefGoogle Scholar
  9. 9.
    Blume A J. Interaction of ligands with the opiate receptors of brain membranes: Regulation by Ions and Nucleotides. Proc Natl Acad Sci USA 75: 1713–1717, 1978.Google Scholar
  10. 10.
    Law P Y, Wu J, KoehlerJ E, Loh H H. Demonstration and characterization of opiate inhibition of the striatal adenylate cyclase. J Neurochem 36: 1834–1846, 1981.PubMedCrossRefGoogle Scholar
  11. 11.
    Attali B, Saya D, Nah S Y, Vogel Z. K-opiate agonists inhibit Ca++ influx in rat spinal cord-dorsal root ganglion cultures. Involvement of a GTP-binding protein. J Biol Chem 264: 347–353, 1989.PubMedGoogle Scholar
  12. 12.
    Dohlman H G, Caron M G, Lefkowitz R J. A family of receptors coupled to guanine nucleotide regulatory proteins. Biochem 26: 2657–2664, 1987.CrossRefGoogle Scholar
  13. 13.
    Chen Y, Mestek A, Liu J, Hurley J A, Yu L. Molecular cloning and functional expression of aµ-opioid receptor from rat brain. Mol Pharmacol 44: 8–12, 1993.PubMedGoogle Scholar
  14. 14.
    Yasuda K, Raynor K, Kong H, Breder C D, Takeda J, Reisine T, Bell G I. Cloning and functional comparison of k and a opioid receptors from mouse brain. Proc Natl Acad Sci USA 90: 6736–6740, 1993.PubMedCrossRefGoogle Scholar
  15. 15.
    Cho T M, Hasegawa J, Ge B L, Loh H H. Purification to apparent homogeneity to aµ-specific opioid receptor from rat brain. Proc Natl Acad Sci 83: 4138–4142, 1986.PubMedCrossRefGoogle Scholar
  16. 16.
    Schofield P R, McFarland K C, Hayflick J S, Wilcox J N, Cho T M Roy S, Lee N M, Loh H H, Seeburg P H. Molecular characterization of a new immunoglobulin superfamily protein with potential roles in opioid binding and cell contact. EMBO J 8: 489–495, 1989.Google Scholar
  17. 17.
    Roy S, Zhu Y X, Lee N M, Loh H H. Different molecular weight forms of opioid receptors revealed by polyclonal antibodies. Biochem Biophys Res Comm 150: 237–244, 1988.PubMedCrossRefGoogle Scholar
  18. 18.
    Roy S, Zhu Y X, Loh H H, Lee N M. A monoclonal antibody that inhibits opioid binding to rat brain membranes. Biochem Biophys Res Comm 154: 688–693, 1988.PubMedCrossRefGoogle Scholar
  19. 19.
    Lane C M, Elde R, Lee N M, Loh H H. Immunofluorescence staining of an OBCAM-like protein resembles the a-opioid receptor in NG108–15 cells. Proc Natl Acad Sci USA 89:1 1 234–1 1 23 8, 1992.Google Scholar
  20. 20.
    Ann D K, Hasegawa J, Ko J L, Chen S T, Lee N M, Loh H H. Specific reduction of a-opioid receptor binding in transfected NG108–15 cells. J Biol Chem 267: 7921–7926, 1992.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Wien 1995

Authors and Affiliations

  • A. P. Smith
    • 1
  • H. H. Loh
    • 1
  1. 1.Department of PharmacologyUniversity of Minnesota Medical School MinneapolisUSA

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