Effects of Cerebrolysin® on cytoskeletal proteins after focal ischemia in rats
In previous studies it was shown that Cerebrolysin® has cerebroprotective effects after moderate global ischemia in rats. In order to investigate mechanisms of these effects, we studied the influence of Cerebrolysin® on dynamics of neurodegeneration of cytoskeletal proteins (MAP2 and alpha-tubulin) after focal brain ischemia in adult male Thomae rats. We used different combinations of ischemia/reperfusion-intervals (2 hrs/90 min and 3 hrs/15 min). Ischemia was induced by a reversible occlusion of the middle cerebral artery by insertion of a nylon monofilament thread. Spreading depressions, ischemic depolarisation, and ECoG were recorded above the core and penumbra of the infarct. CBF (cortical blood flow) was measured in the same regions by a two channel Laser Doppler flowmeter. After fixation procedure and paraffin embedding, the brains were sectioned in 24 slices, each 0.5 mm thick. Slices (5µm) from each plane were used for immunohistochemical staining against MAP2 and alpha-tubulin. The size of the infarction zone and the penumbra was calculated morphometrically. First results indicate that Cerebrolysin® may stabilize cytoskeletal structures. More details will be presented.