Allogeneic Bone Grafts: Study of Radiation Sterilized Bone Tissue by Electron Paramagnetic Resonance Spectrometry and a New Model of Periosteal Induction of Osteogenesis
The positive clinical result of bone grafting depends on rebuilding of the graft i.e. its resorption and induction of new bone formation by the host. In our research on induction of bone formation a new experimental system was described based on induction of periosteal bone formation in mice by Moloney virus. No direct clinical application is proposed.
The fate of radiation sterilized bone grafts can be controlled by a new type of biological marker. This marker is induced by ionizing radiation in the form of stable paramagnetic centers in the crystalline lattice of bone hydroxyapatite. These can be measured by electron paramagnetic resonance (EPR) spectrometry and can serve as biological markers for the evaluation of crystallinity of bone mineral, quantitation of the rebuilding process of radiation sterilized bone grafts and estimation of doses of absorbed ionizing radiation.
KeywordsElectron Paramagnetic Resonance Bone Graft Paramagnetic Center Tooth Enamel Average Crystal Size
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