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Edema Formation Exacerbates Neurological and Histological Outcomes After Focal Cerebral Ischemia in CuZn-Superoxide Dismutase Gene Knockout Mutant Mice

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Brain Edema X

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 70))

Summary

In a variety of studies, CuZn-superoxide dismutase (CuZn-SOD) has been shown to protect against ischemic brain injury. A possible role for CuZn-SOD-related modulation of neuronal viability has been suggested by the finding that CuZn-SOD inhibits brain edema formation following various kinds of neurological insults. We have evaluated the role of CuZn-SOD on brain edema formation following focal cerebral ischemia in mice bearing a disruption of the CuZn-SOD gene (Sod1). Homozygous mutants (Sod1-/-) had no detectable CuZn-SOD activity and heterozygous mutants (Sod1+/-) showed a 50% decrease compared to wild-type mice. Sod1-/- mice showed a high level of blood-brain barrier (BBB) disruption shortly after 1 hr of middle cerebral artery occlusion and 100% mortality at 24 hr following ischemia. Sod1+/- mice showed a moderate level of BBB disruption and 30% mortality. The Sod1+/- animals had increased infarct volume and brain swelling, accompanying exacerbated neurological deficits at 24 hr following ischemia. These results indicate the important role of superoxide anions in the development of brain edema after focal cerebral ischemia and suggest the possibility that brain edema formation may contribute to the exacerbation of ischemic brain injury and neurological deficits in knockout mutant mice.

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Authors and Affiliations

  1. Departments of Neurological Surgery and Neurology, University of California, Box 0651, San Francisco, CA, 94143, USA

    T. Kondo, K. Murakami, S. Chen & Dr. P. H. Chan

  2. Departments of Neurology, University of California, San Francisco, CA, USA

    T. Kondo, K. Murakami, S. Chen & Dr. P. H. Chan

  3. Departments of Neurology Pediatrics,School of Medicine, University of California, San Francisco, CA, USA

    T.-T. Huang, E. Carlson & C. J. Epstein

  4. Departments of Cephalon, Inc., West Chester, PA, USA

    A. G. Reaume & R. W. Scott

Authors
  1. T. Kondo
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  2. A. G. Reaume
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  3. T.-T. Huang
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  4. K. Murakami
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  5. E. Carlson
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  6. S. Chen
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  7. R. W. Scott
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  8. C. J. Epstein
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  9. Dr. P. H. Chan
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Editor information

Editors and Affiliations

  1. Pediatric Neurosurgery, San Diego, CA, USA

    Hector E. James M.D.

  2. Division of Neurological Surgery, San Diego Medical Center, University of California, San Diego, CA, USA

    Lawrence F. Marshall M.D.

  3. Institut für Neurochirurgie, Klinikum Grosshadern, Ludwig-Maximilians-Universität, München, Germany

    Hans J. Raulen (Prof. Dr.) (Prof. Dr.)

  4. Institut für Chirurgische Forschung, Klinikum Grosshadern, Ludwig-Maximilians-Universität, München, Germany

    Alexander Baethmann (Prof. Dr.) (Prof. Dr.)

  5. Neurological Surgery, Virginia Commonwealth University, Richmond, VA, USA

    Anthony Marmarou Ph.D.

  6. Department of Neurosurgery, Musashino Red Cross Hospital, Tokyo, Japan

    Umeo Ito M.D.

  7. Taubmann Health Care Center, The University of Michigan, Ann Arbor, MI, USA

    Julian T. Hoff M.D.

  8. Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    Toshihiko Kuroiwa M.D.

  9. Department of Neurosurgery Medical Resarch Center, Polish Academy of Sciences, Warsaw, Poland

    Zbigniew Czernicki M.D., Ph.D.

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© 1997 Springer-Verlag

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Kondo, T. et al. (1997). Edema Formation Exacerbates Neurological and Histological Outcomes After Focal Cerebral Ischemia in CuZn-Superoxide Dismutase Gene Knockout Mutant Mice. In: James, H.E., et al. Brain Edema X. Acta Neurochirurgica Supplements, vol 70. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6837-0_19

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  • DOI: https://doi.org/10.1007/978-3-7091-6837-0_19

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7418-0

  • Online ISBN: 978-3-7091-6837-0

  • eBook Packages: Springer Book Archive

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