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Leukocyte-Endothelium Interactions in Global Cerebral Ischemia

  • Conference paper
Brain Edema X

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 70))

  • 220 Accesses

Summary

A closed cranial window was implanted in male Mongolian Gerbils to investigate leukocyte-endothelium interactions (LEI) at the brain surface in global cerebral ischemia (GCI) by intravital fluorescence microscopy. Four days after 15 min of bilateral common carotid artery occlusion the ischemic tissue damage was histologically analysed in selectively vulnerable areas of the brain. The frequency of Rhodamine 6G labeled leukocytes rolling along (“roller”) and firmly attached (“sticker”) to postcapillary endothelium was assessed before and up to 180 min after GCI. As compared to the sham operated control animals induction of LEI was found in animals with GCI, following a steady increase up to a significant level attained at 60 min (rollers) or at 120 min of reperfusion (sticker), respectively (p < 0.05). In animals with cerebral ischemia histological assessment revealed a significant decrease of viable neurons in the CA 1 -sector of hippocampus (neurons/mm2 ± SEM: 1308 + 71 vs. 829 ± 106), in parietal neocortex (727 ± 17 vs. 542 ± 49), and in striatum (547 ± 26 vs. 352 ± 49; p < 0.01), respectively. A significant correlation between the extent of irreversible neuronal damage and the frequency of leukocyte adherence to the endothelium could not be established. Nevertheless a direct correlation between the number of surviving neurons and of rolling leukocytes was observed, which may be suggestive of a protective potential of leukocyte rolling.

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Author information

Authors and Affiliations

  1. Institute for Surgical Research, Grosshadern University Hospital, Ludwig-Maximilians-University Munich, Germany

    J. Beck, J. Lehmberg & A. Baethmann

  2. Department of Neurosurgery, Grosshadern University Hospital, Ludwig-Maximilians-University Munich, Marchioninistr. 15, D-81366, Munich, Germany

    W. Stummer & E. Uhl M.D.

Authors
  1. J. Beck
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  2. W. Stummer
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  3. J. Lehmberg
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  4. A. Baethmann
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  5. E. Uhl M.D.
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Editor information

Editors and Affiliations

  1. Pediatric Neurosurgery, San Diego, CA, USA

    Hector E. James M.D.

  2. Division of Neurological Surgery, San Diego Medical Center, University of California, San Diego, CA, USA

    Lawrence F. Marshall M.D.

  3. Institut für Neurochirurgie, Klinikum Grosshadern, Ludwig-Maximilians-Universität, München, Germany

    Hans J. Raulen (Prof. Dr.) (Prof. Dr.)

  4. Institut für Chirurgische Forschung, Klinikum Grosshadern, Ludwig-Maximilians-Universität, München, Germany

    Alexander Baethmann (Prof. Dr.) (Prof. Dr.)

  5. Neurological Surgery, Virginia Commonwealth University, Richmond, VA, USA

    Anthony Marmarou Ph.D.

  6. Department of Neurosurgery, Musashino Red Cross Hospital, Tokyo, Japan

    Umeo Ito M.D.

  7. Taubmann Health Care Center, The University of Michigan, Ann Arbor, MI, USA

    Julian T. Hoff M.D.

  8. Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    Toshihiko Kuroiwa M.D.

  9. Department of Neurosurgery Medical Resarch Center, Polish Academy of Sciences, Warsaw, Poland

    Zbigniew Czernicki M.D., Ph.D.

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© 1997 Springer-Verlag

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Beck, J., Stummer, W., Lehmberg, J., Baethmann, A., Uhl, E. (1997). Leukocyte-Endothelium Interactions in Global Cerebral Ischemia. In: James, H.E., et al. Brain Edema X. Acta Neurochirurgica Supplements, vol 70. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6837-0_16

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  • DOI: https://doi.org/10.1007/978-3-7091-6837-0_16

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-7418-0

  • Online ISBN: 978-3-7091-6837-0

  • eBook Packages: Springer Book Archive

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