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Forschung ohne Tierversuche 1996 pp 88–98Cite as

Embryonale Stammzellen als Modellobjekt der Entwicklungsgenetik und Reproduktionsbiologie

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Part of the book series: Ersatz- und Ergänzungsmethoden zu Tierversuchen ((TIERVERSUCHE))

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Literatur

  • BRadley A., EVans M., KAufman M.H., RObertson E., Formation of germ-line chimearas from embryo-derived teratocarcinoma cell lines, Nature, 309, 255–256, 1984

    Article  PubMed  CAS  Google Scholar 

  • EVans M.J. and KAufman M.H., Establishment in culture of pluripotential stem cells from mouse embryos, Nature, 291, 154–156, 1981

    Article  Google Scholar 

  • DOetschmann T.C., EIstetter H.R., KAtz M., SChmidt W., KEmler R., The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolc sac, blood islands and myocardium, 1 Embryol. Exp. Morph., 87, 27–45, 1985

    Google Scholar 

  • FāSsler R. and MEyer M., Consequences of lack of bl integrin gene expression in mice, Genes & Dev., 1995, in press

    Google Scholar 

  • FāSsler R., ROhwedel J., MAltsev V., BLoch W., LEntini S., GUllberg D., HEscheler J., ADdicks K., WObus A.M., Cardiac muscle cell differentiation and sarcomere integrity are impaired in the absence of ßl integrin, eingereicht

    Google Scholar 

  • FRiedberg E.C., BArdwell A.J., BArdwell L., ZHigang WAng, DIanov G., Transcription and nucleotide excision repair - reflections, considerations and recent biochemical insights, Mut. Res., 307, 5–14, 1994

    CAS  Google Scholar 

  • KLeppisch T., WObus A.M., STrüBing C, HEscheler J., Voltage-dependent L-type Ca channels and a novel type of non-selective cation channel activated by cAMP-dependent phosphorylation in mesoderm-like (MES-1) cells, Cellular Signalling, 5, 727–734, 1993

    Article  PubMed  CAS  Google Scholar 

  • LAschinski G., VOgel R., SPIelmann H., Cytotoxicity test using blastocyst-derived euploid embryonal stem cells: A new approach to in vitro teratogenesis screening, Reprod. Toxicology, 5, 57–64, 1991

    CAS  Google Scholar 

  • MAltsev V.A., ROhwedel J., HEscheler J., WObus A.M., Embryonic stem cells differentiate in vitro into cardiomyocytes representing sinusnodal, atrial and ventricular cell types, Mech. Dev., 44, 41–50, 1993

    Article  PubMed  CAS  Google Scholar 

  • MAltsev V. A., WObus A.M., ROhwedel J., BAderm., HEscheler J., Cardiomyocytes differentiated in vitro from embryonic stem cells developmentally express cardiac-specific genes and ionic currents, Circ. Res., 75, 233–244, 1994

    PubMed  CAS  Google Scholar 

  • MArtin G., Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma cells, Proc. Natl. Acad. Sci. USA, 78, 7634–7638, 1981

    Article  PubMed  CAS  Google Scholar 

  • MArtin G., Teratocarcinomas and mammalian embryogenesis, Science, 209, 768–776, 1980

    Article  PubMed  CAS  Google Scholar 

  • MAtsui Y., ZSebo K., HOgan B.L.M., Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture, Cell, 70, 841–847, 1992

    Article  PubMed  CAS  Google Scholar 

  • MCburney M.W. and ROgers B.J., Isolation of male embryonal carcinoma cells and their chromosome replication patterns, Dev. Biol., 89, 503–508, 1982

    Article  PubMed  CAS  Google Scholar 

  • MOnk M., Changes in DNA methylation during mouse embryonic development in relation to X-chromosome activity and imprinting, Phil. Trans. R. Soc. Lond B, 326, 299–312, 1990

    Article  CAS  Google Scholar 

  • MUmmery C.L., FEijen A., Van Der SAag P.T., Van Den BRink C.E., De LAat S. W., Clonal variants of differentiated PI9 embryonal carcinoma cells exhibit epidermal growth factor receptor kinase activity, Dev. Biol., 109, 402–410, 1985

    Article  PubMed  CAS  Google Scholar 

  • MUmmery C.L., FEijen A, Van Den BRink C.E., MOolenaar, W.H., De LAat S.W., Establishment of a differentiated mesodermal line from PI9 EC cells expressing functional PDGF and EGF receptors, Exp. Cell Res., 165, 229–242, 1986

    Article  PubMed  CAS  Google Scholar 

  • NEwalld.R. and BEedles K.E., The stem-cell test - a novel in vitro assay for teratogenic potential, Toxicol, in Vitro, 8 697–701, 1994

    Article  CAS  Google Scholar 

  • RAsko I., GEorgieva M., FArkas G., SAntha M., COates J., BUrg K., MItchell D.L., JOhnson R.T., New pattern of bulk DNA repair in ultraviolet irradiated mouse embryo carcinoma cells following differentiation, Somat. Cell Mol. Genet., 19, 245–255, 1993

    Article  PubMed  CAS  Google Scholar 

  • RIsau W., SAriola H., ZErwes H.-G., SAsse J., EKblom P., KEmler R., DOetschman T., Vasculogenesis and angiogenesis in embryonic stem cell-derived embryoid bodies, Development, 102, 471–478, 1988

    PubMed  CAS  Google Scholar 

  • RObertson E., Embryo-derived stem cell lines, in: ROBERTSON E.J. (ed.), Teratocarcinomas and embryonic stem cells: a practical approach, Oxford: IRL Press, 71–112, 1987

    Google Scholar 

  • ROhwedel J., MAltsev V., BOber E., ARnold H.-H., HEscheler J., WObus A.M., Muscle cell differentiation of embryonic stem cells reflects myogenesis in vivo: Developmentally regulated expression of myogenic determination genes and functional expression of ionic currents, Dev. Biol., 164, 87–102, 1994

    Article  PubMed  CAS  Google Scholar 

  • ROhwedel J., HOrak V., HEbrok M., Füchtbauer E.-M., WObus A.M., M-twist expression inhibits mouse embryonic stem cell-derived myogenic differentiation in vitro, Exp. Cell Res., 220, 92–100, 1995

    Article  PubMed  CAS  Google Scholar 

  • ROse O., ROhwedel J., REinhard S., BAchmann M., CRamer M., ROtter M., WObus A.M., STarzinski-POwitz A., Expression of M-cadherin protein in myogenic cells during prenatal mouse development and differentiation of embryonic stem cells in culture, Dev. Dynamics, 201, 245–259, 1994

    Article  CAS  Google Scholar 

  • SCholer H.R., HAtzopoulos A.K., BAlling R., SUzuki N., GRuss P., A family of octamer-specific proteins present during mouse embryogenesis: evidence for germline-specific expression of an Oct factor, EMBO J., 8, 2543–2550, 1989

    PubMed  Google Scholar 

  • SEhlmeyer U. and WObus A.M., Lower mutation frequencies are induced by ENU in undifferentiated embryonic cells than in differentiated cells of the mouse in vitro, Mutation Res., 324,69–76, 1994

    Article  PubMed  CAS  Google Scholar 

  • SEhlmeyer U. and WObus A.M., MNNG is less cytotoxic and mutagenic in undifferentiated embryonic cells than in differentiated cells of the mouse in vitro, In Vitro Toxicol., 8, 121–127, 1995

    CAS  Google Scholar 

  • SEhlmeyer U., MEister A., BEisker W., WObus A.M., The lower efficiency of mitomycin C to induce gene mutations and micronuclei in undifferentiated embryonic cells in vitro is correlated with efficient cell cycle control, eingereicht

    Google Scholar 

  • STewart C., GAdi I., BHatt H., Stem cells from primordial germ cells can reenter the germ line, Dev. Biol., 161, 626–628, 1994

    Article  PubMed  CAS  Google Scholar 

  • STrüBing C., AHnert-HIlger G., JIn SHan, WIedenmann B., HEscheler J., WObus A.M., Differentiation of pluripotent embryonic stem cells into the neuronal lineage in vitro gives rise to mature inhibitory and excitatory neurons, Mech. Dev., 53,1–13, 1995

    Google Scholar 

  • THomas K.R. and Capecchi, Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells, Cell, 51, 503–512, 1987

    Article  PubMed  CAS  Google Scholar 

  • TLsty T.D., BRiot A., GUalberto A., HAll I., HEss S., HIxon M., KUppuswamy D., ROmanov S., SAgem., WHite A., Genomic instability and cancer, Mutation Res., 337, 1–7, 1995

    Article  PubMed  CAS  Google Scholar 

  • WIles M.V. and KEller G., Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture, Development, 111, 259–267, 1991

    PubMed  CAS  Google Scholar 

  • WIles M.V., Embryonic stem cell differentiation in vitro, Methods Enzymol., 225, 900–918, 1993

    Article  PubMed  CAS  Google Scholar 

  • WObus A.M., WAllukat G., HEscheler J., Pluripotent mouse embryonic stem cells are able to differentiate into cardiomyocytes expressing chronotropic responses to adrenergic and cholinergic agents and Ca2+ channel blockers, Differentiation, 48,173–182, 1991

    Article  PubMed  CAS  Google Scholar 

  • WObus A.M., KLeppisch T., MAltsev V., HEscheler J., Cardiomyocyte-like cells differentiated in vitro from embryonic carcinoma cells P19 are characterized by functional expression of adrenoceptors and Ca2+ channels, In Vitro Cell. Dev. Biol., 30A, 425–434, 1994a

    Article  Google Scholar 

  • WObus A.M., ROhwedel J., MAltsev V., HEscheler J., In vitro differentiation of embryonic stem cells into cardiomyocytes or skeletal muscle cells is specifically modulated by retinoic acid, Roux’s Arch. Dev. Biol., 204, 36–45, 1994b

    Article  CAS  Google Scholar 

  • WObus A.M., ROhwedel J., MAltsev V., HEscheler J., In vitro cellular models for cardiac development and pharmacotoxicology, Toxicol, in Vitro, 9, 477–488, 1995

    Article  CAS  Google Scholar 

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Authors
  1. A. M. Wobus
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  2. J. Rohwedel
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  3. U. Sehlmeyer
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Editor information

Editors and Affiliations

  1. zet-Zentrum für Ersatz und Ergänzungsmethoden zu Tierversuchen, A-Linz, Austria

    Harald Schöffl  & Helmut A. Tritthart  & 

  2. ZEBET-Zentralstelle zur Erfassung und Bewertung von Ersatz- und Ergänzungsmethoden zum Tierversuch im BgVV - Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, D-Berlin, Germany

    Horst Spielmann

  3. Institut für Medizinische Physik und Biophysik, Karl-Franzens-Universität, A-Graz, Austria

    Helmut A. Tritthart

  4. PEI — Paul-Ehrlich-Institut, D-Langen, Germany

    Klaus Cußler

  5. Stiftung für das Tier im Recht, CH-Zürich, Switzerland

    Antoine F. Goetschel

  6. FFVFF — Fonds für versuchstierfreie Forschung, CH-Zürich, Switzerland

    Franz P. Gruber

  7. SAAT—Swiss Alternatives to Animal Testing, CH-Bertschikon-Zürich, Switzerland

    Christoph A. Reinhardt

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© 1997 Springer-Verlag/Wien

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Wobus, A.M., Rohwedel, J., Sehlmeyer, U. (1997). Embryonale Stammzellen als Modellobjekt der Entwicklungsgenetik und Reproduktionsbiologie. In: Schöffl, H., et al. Forschung ohne Tierversuche 1996. Ersatz- und Ergänzungsmethoden zu Tierversuchen. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6833-2_12

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  • DOI: https://doi.org/10.1007/978-3-7091-6833-2_12

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82869-4

  • Online ISBN: 978-3-7091-6833-2

  • eBook Packages: Springer Book Archive

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