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Serological and virological responses in mules and donkeys following inoculation with African horse sickness virus serotype 4

  • H. El Hasnaoui
  • M. El Harrak
  • S. Zientara
  • M. Laviada
  • C. Hamblin
Conference paper

Summary

Two groups, comprising 4 donkeys and 4 mules (group 1) and 4 donkeys and 3 mules (group 2), were used to determine the duration of viraemia and to monitor the development of antibodies following inoculation with African horse sickness virus (AHSV). One group of animals was given a single dose of attenuated AHSV serotype 4 (AHSV 4) vaccine. The second group was inoculated with a virulent field strain of AHSV 4. Both groups were subsequently challenged with the virulent field strain of AHSV 4, 51 and 58 days, respectively, after their primary inoculation. Blood and serum samples, collected on alternate days after the primary inoculations and also after subsequent challenge, were assayed for virus and antibodies. Seven of the 8 AHSV vaccinated (group 1) and 7 of the 7 AHSV inoculated (group 2) animals showed humoral antibody responses after primary inoculation. Although no infectious virus could be isolated from either group for the duration of the study, reverse transcription-PCR data obtained for the second group did show the presence of AHSV viral RNA from as early as day 5 in mules and day 9 in donkeys after the primary inoculation. Viral RNA was detected consistently up to day 47 in some animals and intermittently thereafter. There was no evidence of a second viraemia in any of the animals after challenge. The detection of specific antibodies, against AHSV 4 NS3 protein, in all animals confirmed that both donkeys and mules were infected and that the virus had replicated.

Keywords

Antibody Titre Virological Response Vaccination Challenge Humoral Antibody Response African Horse Sickness Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Baylis M, Hasnaoni H, Bouayoune H, Touti J, Mellor PS (1997) The spacial and seasonal distribution of African horse sickness and its potential Culicoides vectors in Morocco. Med Vet Entomol 11: 203–212PubMedCrossRefGoogle Scholar
  2. 2.
    Hamblin C, Graham SD, Anderson EC, Crowther JC (1990) A competitive ELISA for the detection of group-specific antibodies to African horse sickness virus. Epidemiol Infect 104: 303–312PubMedCrossRefGoogle Scholar
  3. 3.
    Hamblin C, Salt JS, Mellor PS, Graham SD, Smith PR, Wohlsein P (1998) Donkeys as a reservoir of African horse sickness. In: Mellor PS, Baylis M, Hamblin C, Calisher CH, Mertens PPC (eds) African horse sickness. Springer, Wien New York, pp 37–47 (Arch Virol [Suppl] 14 )Google Scholar
  4. 4.
    Hasnaoui H, Harrak M, Tber A, Fikri A, Laghzaoui K, Bikour MH (1998) Application of an indirect fluorescent antibody assay for the detection of African horse sickness virus antibodies. In: Mellor PS, Baylis M, Hamblin C, Calisher CH, Mertens PPC (eds) African horse sickness. Springer, Wien New York, pp 29–36 (Arch Virol [Suppl] 14 )Google Scholar
  5. 5.
    Lhafi A, Tber A, Fikri A, Laghzaoui K (1992) African horse sickness in Morocco: the epizootics of 1989 and 1990. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proc 2nd Int Symp. CRC Press, Boca Raton, pp 205–216Google Scholar
  6. 6.
    Laviada MD, Roy P, Vizcaino-Sanchez JM, Casal JI (1995) The use of African horse sickness virus NS3 protein, expressed in bacteria, as a marker to differentiate infected from vaccinated horses. Virus Res 38: 205–218PubMedCrossRefGoogle Scholar
  7. 7.
    Venter GJ, Williams R, Nevill EM (1992) The value of donkeys as sentinels for African horse sickness. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proc 2nd Int Symp. CRC Press, Boca Raton, pp 825–831Google Scholar
  8. 8.
    Zientara S, Sailleau C, Moulay S, Wade-Evans A, Cruciere C (1995) Application of the polymerase chain reaction to the detection of African horse sickness viruses. J Virol Methods 53: 47–54PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag/Wien 1998

Authors and Affiliations

  • H. El Hasnaoui
    • 1
  • M. El Harrak
    • 2
  • S. Zientara
    • 3
  • M. Laviada
    • 4
  • C. Hamblin
    • 5
  1. 1.Laboratoire Régional d’Analyses et de Recherches VétérinairesCasablancaMorocco
  2. 2.Laboratoire BiopharmaRabat-AkkariMorocco
  3. 3.Centre National d’Etudes Veterinaires et AlimentairesLaboratoire Central de Recherches VétérinairesMaisons-AlfortFrance
  4. 4.Centro de Investigacion en Sanidad Animal Instituto Nacional de Investigacion y Technologia Agraria AlimentariaMadridSpain
  5. 5.Institute for Animal HealthPirbright LaboratoryWoking, SurreyUK

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