Application of an indirect fluorescent antibody assay for the detection of African horse sickness virus antibodies
An indirect fluorescent antibody (IFA) technique was used to screen and quantify antibodies against African horse sickness virus (AHSV) in equine sera. Results obtained with the IFA assay were compared directly with those obtained with standard complement fixation (CF) and virus neutralisation (VN) tests using horse sera from experimental studies and samples from the field. Positive fluorescent antibody titres were detected from as early as 7 days after primary vaccination and persisted for at least six months. The IFA technique offers a clear advantage over CF tests, where the antibodies are often of shorter duration and where sera from donkeys and mules are frequently anticomplementary. The sensitivity and specificity of the IFA test compared with the VN test were 98% and 83.3%, respectively. The IFA test is rapid, relatively easy to perform and inexpensive, and can be recommended as an alternative assay for screening different species of equidae in AHSV control and surveillance programmes.
KeywordsComplement Fixation Complement Fixation Test Indirect Fluorescent Antibody Test Indirect Fluorescent Antibody African Horse Sickness Virus
Unable to display preview. Download preview PDF.
- 3.Dubourget P, Preaud JM, Detraz N, Lacoste F, Fabry AC, Erasmus B, Lombard M (1992) Development, production and quality control of an industrial inactivated vaccine against AHS type IV. In: Walton TE, Osburn BI (eds) Bluetongue, African horse sickness and related orbiviruses. Proc 2nd Int Symp. CRC Press, Boca Raton, pp 874–886Google Scholar
- 4.Hazrati A, Mirchamsy H, Bahrami S (1972) Comparative studies on the serological responses of horses to African horse-sickness virus. In: Bryans JT, Gerber H (eds) Equine infectious diseases III. Proc 3rd Int Con Equine Inf Dis. Karger, Basel, pp 69–80Google Scholar
- 5.Hermiman KAJ, Gumm ID, Owen L, Taylor WP, Sellers RF (1980) Distribution of bluetongue virus and antibodies in some countries of the eastern hemisphere. Bull Off Int Epiz 92: 581–586Google Scholar
- 6.Holmes HI, Boccardo G, Estes MK, Furuichi MK, Hoshino Y, Joklik WK, Mc Crae M, Mertens PPC, Milne RG, Samal KSK, Shikata E, Winton JR, Uyeda I, Nuss DL (1995) Family Reoviridae. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus Taxonomy. Classification and Nomenclature of Viruses. Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 208–239 (Arch Virol [Suppl] 10 )Google Scholar
- 7.Howell PG (1963) African horse sickness. In: Emerging diseases of animals. Food and Agric Org, Rome No 61, 73–108Google Scholar