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Structural studies of orbivirus particles

  • D. I. Stuart
  • P. Gouet
  • J. Grimes
  • R. Malby
  • J. Diprose
  • S. Zientara
  • J. N. Burroughs
  • P. P. C. Mertens
Conference paper

Summary

We are using crystallographic methods to investigate the structure of AHSV and BTV. Our initial approach was to investigate the structure of the major protein component of the viral core, VP7(T13). This trimeric protein has been studied in several crystal forms from both orbiviruses and reveals a structure made up of conserved domains, capable of conformational changes and possessing a cleavage site. Further crystallographic analyses of native particles have provided a picture of the VP7(T13) and VP3(T2) layers of the BTV core. The VP7(T13) layer consists of 260 trimers arranged rather symmetrically and possessing very similar structures, thereby following the rules of quasi equivalence. The VP3(T2) layer is thin and contains 120 copies of 100 kDa protein arranged as 60 approximate dimers. This type of icosahedral construction has not been observed before and appears to contain a genome which is highly ordered. We anticipate that all of these features will be common to AHSV.

Keywords

Core Particle Apical Domain Cryoelectron Microscopy European Synchrotron Radiation Facility Bluetongue Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag/Wien 1998

Authors and Affiliations

  • D. I. Stuart
    • 1
    • 2
  • P. Gouet
    • 1
  • J. Grimes
    • 1
  • R. Malby
    • 1
  • J. Diprose
    • 1
  • S. Zientara
    • 3
  • J. N. Burroughs
    • 4
  • P. P. C. Mertens
    • 5
  1. 1.Laboratory of Molecular BiophysicsOxfordUK
  2. 2.New Chemistry LaboratoryThe Oxford Centre for Molecular SciencesOxfordUK
  3. 3.Laboratoire Central de Recherches VétérinairesCentre National d’Études Vétérinaires et AlimentairesMaisons-AlfortFrance
  4. 4.Pirbright LaboratoryInstitute for Animal HealthPirbright, SurreyUK
  5. 5.Pirbright LaboratoryInstitute for Animal HealthPirbright, Woking SurreyUK

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