Summary
The human and animal cell lines are a very promising tool for complementing and in perspective substituting the in vivo methods, at least in some biomedical disciplines as toxicology and cosmetology. The quality and reproducibility of in vitro assays largely depend on a deep knowledge of the cell line features and on the guarantee of operating with certified material. The cell line collections arose around the world in response to this need for well characterised material, free of cellular and microbial contaminants.
In Italy, only very recently the researchers have started to be aware of the need for quality control of the cell lines used in their experiments. In this frame, the Interlab Cell Line Collection (ICLC) has been set up in 1994 in Genoa, at the Advanced Biotechnology Center. The activity of ICLC includes: collection, production, quality control, characterisation, expansion, storage and distribution of human and animal cell lines and hybridomas.
Zusammenfassung
Humane und tierische Zellinien sind besonders in Toxikologie und Kosmetik vielversprechende Ersatz- und Ergänzungsmethoden für in vivo-Methoden. Die Qualität und Reproduzierbarkeit von in vitro-Versuchen hängen stark von einem profunden Wissen über die Eigenschaften der Zellinien ab und von der Garantie, mit zertifiziertem Material zu arbeiten. Zelliniensammlungen entstanden weltweit als Antwort auf den Bedarf nach charakterisiertem, kontaminationsfreiem Material.
In Italien wurden sich die Forscher erst kürzlich dieses Bedarfes bewußt. Daher wurde die Interlab Cell Line Collection (ICLC) 1994 am Advanced Biotechnology Center in Genoa gegründet. Die ICLC beschäftigt sich mit der Sammlung, Produktion, Qualitätskontrolle, Charakterisierung, Erweiterung, Lagerung und Verteilung von humanen und tierischen Zelllinien und Hybridlinien.
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Parodi, B., Aresu, O., Visconti, P., Maggi, E., Parodi, A.M., Ruzzon, T. (2000). Authentication and quality control of cell lines at the Interlab Cell Line Collection. In: Schöffl, H., et al. Forschung ohne Tierversuche 2000. Ersatz- und Ergänzungsmethoden zu Tierversuchen. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6760-1_37
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DOI: https://doi.org/10.1007/978-3-7091-6760-1_37
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