Zusammenfassung
Ein in vitro-Mikrokerntest mit der permanenten Fischzellinie RTG-2 wurde entwickelt und standardisiert, um industrielle Abwasserproben auf ihr gentoxisches Potential hin untersuchen zu können. Da die Durchflußzytometrie die Auswertung einer großen Anzahl von Proben unter standardisierten und objektiven Bedingungen erlaubt, wurden zunächst Vergleichsuntersuchungen der durchflußzytometrischen Messung mit der mikroskopischen Auswertung durchgeführt. Für die drei geprüften Referenzsubstanzen ergaben sich vergleichbare Dosiswirkungskurven. Bei der folgenden durchflußzytometrischen Messung von 38 industriellen Abwasserproben aus 11 verschiedenen Industriesparten zeigten 14 Proben einen Anstieg der Mikrokernhäufigkeit. Von diesen gentoxischen Abwasserproben stammten 7 von metallverarbeitenden Betrieben. Diese Ergebnisse belegen die Notwendigkeit, das gentoxische Potential von Abwässern zu prüfen, um eine bessere Risikoabschätzung für die einzelnen Industriesparten zu ermöglichen.
Summary
An in vitro micronucleus assay using the permanent fish cell line RTG-2 was developed and standardized to test industrial waste waters for their genotoxic potential. Because of the fact that flow cytometry allows measure a great number of samples under standardized and objective conditions in a first Step the flow cytometric measurement of micronuclei were compared with microscopic counting. All three tested reference chemicals showed similar dose effect relationships in both systems. In the following flow cytometric measurement of 38 industrial waste waters from 11 different industrial branches 14 showed an increase of the micronucleus frequency. From these genotoxic waste waters 7 originate from metal industries. These results demonstrate the necessity to assess the genotoxic potential of waste waters to calculate the ecological risk from each industrial branch.
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Kohlpoth, M. (2000). Durchflußzytometrische Messung von induzierten Mikrokernen in einer permanenten Fischzellinie zur Erfassung des gentoxischen Potentials von industriellen Abwässern. In: Schöffl, H., et al. Forschung ohne Tierversuche 2000. Ersatz- und Ergänzungsmethoden zu Tierversuchen. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6760-1_28
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DOI: https://doi.org/10.1007/978-3-7091-6760-1_28
Publisher Name: Springer, Vienna
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