Zusammenfassung
Verfahren zur Messung der Wund- und Nahtfestigkeit sind wichtige Bestandteile der Wundheilungsforschung. Als Standardtiermodell gilt die Ratte, gefolgt von Kaninchen, Meerschweinchen und Maus.
Es wird ein Verfahren vorgestellt, das ohne bzw. ergänzend zu Tierversuchen Festigkeitsprüfungen ermöglicht. Der Versuchsaufbau umfaßt eine Universalprüfmaschine und Umlenkprüfklemmen nach der Deutschen Industrie Norm 53455. Die hanteiförmigen Zugproben bestehen aus einem feinmaschigen Textilstoff und tragen in der Mitte die zu testenden Nähte. Als Meßbeispiel wird die Bruchfestigkeit von resorbierbaren Subkutannähten vor und nach ihrer Resorbtion im subkutanen Gewebe untersucht. Die Resorbtion des Nahtmaterials wird durch Inkubation der gesamten Probe in einem definierten Degradationsbad bei 50°C und für 96 Stunden simuliert. Die Bruchfestigkeit (N/cm) der Nähte wurde vor und nach der simulierten Resorption im Zugversuch bestimmt. Die Ergebnisse zeigen, daß ohne Tierversuche zuverlässige, für den chirurgischen Alltag relevante Festigkeitsanalysen von Gewebeverbindungen möglich sind.
Summary
Methods to determine the mechanical properties of surgical wounds and sutures are valuable tools in wound healing research. The rat serves as the standard animal model, followed by rabbits, guinea pigs and mice.
As an additional option to experiments with living or sacrified animals, a method without animals is suggested. The experimental design follows German industrial standards (DIN 53455). It includes an universal testing-machine and special clamps. The dumbell-shaped specimen consist of a standardised textile material and the suture, which are to be tested. As an example the breaking strength of resorbable subcutaneous sutures is measured. The resorbtion of the suture material is simulated by the incubation of the specimen in a degrading bath. Breaking-strength is analysed before and after degrading the suture material. The results show the method allows reproducible analysis of surgical sutures without using animals.
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Bücheler, M. (2000). Wund- und Nahtfestigkeitsprüfung am Tierersatzmodell. In: Schöffl, H., et al. Forschung ohne Tierversuche 2000. Ersatz- und Ergänzungsmethoden zu Tierversuchen. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6760-1_2
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DOI: https://doi.org/10.1007/978-3-7091-6760-1_2
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