Identification of Fungal Genes Involved in Plant Pathogenesis and Host Range

  • Willi Schäfer
  • Dietmar Stahl
  • Enrico Mönke
Part of the Plant Gene Research book series (GENE)


The application of molecular genetic techniques to study the interactions of a fungal pathogen with its host plant is just beginning. Nevertheless a number of encouraging results have already emerged and they highlight the power of this approach. In this chapter we will describe some of these techniques and demonstrate with a few selected examples how the application of molecular biology can provide answers to long-standing questions.


Fungal Gene Aspergillus Nidulans Pectate Lyase Fusarium Solani Papaya Fruit 


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  1. Andrews E.C., Hanowski C., Beiderbeck R. (1981) Wachtumsveränderungen an Maiskeimlingen nach Befall mit haploiden Linien des Maisbrandes (Ustilago maydis). Phytopathol Z 102: 1O–20Google Scholar
  2. Armaleo D., Ye G.-N., Klein T.M., Shark K.B., Sanford J.C., Johnston S.A. (1990) Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi. Curr Genet 17: 97–103PubMedCrossRefGoogle Scholar
  3. Avalos J., Geever R.F., Case M.E. (1989) Bialaphos resistance as a dominant selectable marker in Neurospora crassa. Curr Genet 16: 369–372PubMedCrossRefGoogle Scholar
  4. Banuett F., Herskowitz L. (1989) Different a-alleles of Ustilago maydis are necessary for maintenance of filamentous growth but not for meiosis. Proc Natl Acad Sci USA 86: 5878–5882PubMedCrossRefGoogle Scholar
  5. Bannuett F., Herskowitz I. (1992) Pathogenicity determinants in the smut fungi of cereals. In: Boller T., Meins F. (eds) Genes involved in plant defense. Springer, Wien New York, pp 115–128 [Dennis E.S. et al (eds) Plant gene research. Basic knowledge and application]Google Scholar
  6. Bej A.K., Perlin M. (1988) Apparent transformation and maintenance in basidiomycete mitochondria of a plasmid bearing the hygromycinB gene. Genome 19 [Suppl 1]: 300Google Scholar
  7. Bell A.A., Wheeler M.H. (1986) Biosynthesis and functions of fungal melanins. Annu Rev Phytopathol 24: 411–451CrossRefGoogle Scholar
  8. Berg D.E., Davies J., Allet B., Rochaix J.-D. (1975) Transposition of R factor genes to bacteriophage lambda. Proc Natl Acad Sci USA 72: 3628–3632PubMedCrossRefGoogle Scholar
  9. Blakemore E.J.A., Dobson M.J., Hocart M.J., Lucas J.A., Peberdy J.F. (1989) Transformation of Pseudocerecosporella herpotrichoides using two heterologous genes. Curr Genet 16: 177–180CrossRefGoogle Scholar
  10. Case M.E., Schweizwer M., Kushner S.R., Giles N.H. (1979) Efficient tranformation of Neurospora crassa by utilizing hybrid plasmid DNA. Proc Natl Acad Sci USA 76: 5259–5263PubMedCrossRefGoogle Scholar
  11. Chumley F.G., Valent B. (1990) Genetic analysis of melanin deficient, nonpathogenic mutants of Magnaporthe grisea. Mol Plant Microbe Interact 3: 135–143CrossRefGoogle Scholar
  12. Churchill A.C.L., Ciuffetti L.M., Hansen D.R., VanEtten H.D., Van Alfen N.K. (1990) Transformation of the fungal pathogen Cryphonectria parasitica with a variety of heterologous plasm ids. Curr Genet 17: 25–31Google Scholar
  13. Ciuffetti L.M., Weltring K.M., Turgeon B.G., Yoder O.C., VanEtten H.D. (1988) Tranformation of Nectria haematococca with a gene for pisatin demethylating activity, and the role of pisatin detoxification in virulence. J Cell Biochem 12C: 278Google Scholar
  14. Cooley R.N., Shaw R.K., Franklin F.C.H., Caten C.E. (1988) Transformation of the phytopathogenic fungus Septoria nodorum to hygromycinB resistance. Curr Genet 13: 383–389CrossRefGoogle Scholar
  15. Crawford M.S., Kolattukudy P.E. (1987) Pectate lyase from Fusarium solani f. sp. pisi: purification, characterization. in vitro translation of the mRNA, and involvement in pathogenicity. Arch Biochem Biophys 258: 196–205PubMedCrossRefGoogle Scholar
  16. Daboussi M.J., Djeballi A., Gerlinger A., Blaiseau P.L., Bouvier I., Cassan M., Lebrun M.H., Parisot D., Brygoo Y. (1989) Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans. Curr Genet 15: 453–456PubMedCrossRefGoogle Scholar
  17. Dantzig A.H., Zuckerman S.H., Andonov-Roland M.M. (1986) Isolation of a Fusarium solani mutant reduced in cutinase activity and virulence. J Bacteriol 168: 911–916PubMedGoogle Scholar
  18. Dean A.R., Timberlake W.E. (1989) Regulation of the Aspergillus nidulans pectate lyase gene (pel A). Plant Cell 1: 275–284PubMedGoogle Scholar
  19. De Wit P.J.G.M. (1992) Functional models to explain gene-for-gene relationships in plant-pathogen interactions. In: Boller T., Meins F. (eds) Genes involved in plant defense. Springer, Wien New York. pp 25–47 [Dennis E.S. et al (eds) Plant gene research. Basic knowledge and application]CrossRefGoogle Scholar
  20. Dickman M.B. (1988) Whole cell transformation of the alfalfa pathogen Colletotrichum trifolii. Curr Genet 14: 241–246CrossRefGoogle Scholar
  21. Dickman M.B., Patil S.S. (1986) Cutinase deficient mutants of Colletotricum gloeosporioides are nonpathogenic to papaya fruit. Physiol Mol Plant Pathol 28: 235–242CrossRefGoogle Scholar
  22. Dickman M.B., Podila G.K., Kolattukudy P.E. (1989) Insertion of cutinase gene into a wound pathogen enables it to infect intact host. Nature 342: 446–448CrossRefGoogle Scholar
  23. Dinoor A., Eshed N., Nof E. (1988) Puccinia coronata, crown rust of oats and grasses. In: Sidhu G.S. (ed) Genetics of plant pathogenic fungi. Academic Press, London, pp 333–344(Advances in plant pathology, vol 6)Google Scholar
  24. Duguid J.R., Rohwer R.G., Seed B. (1988) Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridisation of a eDNA library. Proc Natl Acad Sci USA 85: 5738–5742PubMedCrossRefGoogle Scholar
  25. Farman M.L., Oliver R.P. (1987) The transformation of protoplasts of Leptoshaeria maculans to hygromycin B resistance. Curr Genet 13: 327–330CrossRefGoogle Scholar
  26. Fincham J.R. (1989) Transformation in fungi. Microbiol Rev 53: 148–170PubMedGoogle Scholar
  27. Froyd J.D., Paget C.J., Guse L.R., Dreikorn B.A., Pafford J.L. (1976) A new systemic fungicide for control of Pyricularia oryzae on rice. Phytopathology 66: 1135–1139CrossRefGoogle Scholar
  28. Fry W.E., Yoder O.C., Apple A.E. (1984) Influence of naturally occurring marker genes on the ability of Cochliobolus heterostrophus to induce field epidemics of southern corn leaf blight. Phytopathology 74: 175–178CrossRefGoogle Scholar
  29. Gatignol A., Baron M., Tiraby G. (1987) Phleomycin resistance encoded by the ble gene from trans po son Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae. Mol Gen Genet 207: 342–348PubMedCrossRefGoogle Scholar
  30. Goldmann G.H., Van Montagu M., Herrera-Estrella A. (1990) Transformation of Trichoderma harzianum by high voltage electric pulse. CUff Genet 17: 169–174CrossRefGoogle Scholar
  31. Gritz L., Davies J. (1983) Plasmid-encoded hygromycin B resistance: the sequence of hygromycinB phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179–188PubMedCrossRefGoogle Scholar
  32. Gysler C., Harmsen J.A.M., Kester H.C.M., Visser J., Heim J. (1990) Isolation and structure of the pectin lyase D-encoding gene from Aspergillus niger. Gene 89: 101–108PubMedCrossRefGoogle Scholar
  33. Hanna W.F. (1929) Studies in the physiology and cytology of Ustilago zeae and Sorosphorium reilianum. Phytopathology 19: 415–442Google Scholar
  34. Heinen W. (1960) Über den enzymatischen Cutinabbau I. Mitteilung: Nachweis eines Cutinase-Systems. Acta Bot Neerl 9: 167–190Google Scholar
  35. Heinen W., de Vries H. (1966) Stages during the breakdown of plant cutin by soil microorganisms. Arch Microbiol 54: 331–338Google Scholar
  36. Henson J.M., Blake N.K., Pilgeram A. (1988) Transformation of Gaeumannomyces graminis to benomyl resistance. CUff Genet 14:113–117CrossRefGoogle Scholar
  37. Hinnen A., Hicks J.R., Fink G.R. (1978) Transformation of yeast chimaeric ColEI plasmid carrying LEU 2. Proc Natl Acad Sci USA 75: 1929–1933PubMedCrossRefGoogle Scholar
  38. Holden D.W., Wang J., Leong S.A. (1988) DNA-mediated transformation of Ustilago hordei and Ustilago nigra. Physiol Mol Plant Pathol 33: 235–239CrossRefGoogle Scholar
  39. Howard R.J., Ferrari M.A. (1989) The role of melanin in appressorium function. Exp Mycol 13: 403–418CrossRefGoogle Scholar
  40. Huang D., Bhairi D., Staples R.C. (1989) A transformation procedure for Botryotinia squamosa. Curr Genet 15: 411–414CrossRefGoogle Scholar
  41. Hynes M.J., Corrick C.M., King J.A. (1983) Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol Cell Biol 3: 1430–1439PubMedGoogle Scholar
  42. Ito H., Fukuda Y., Murata K., Kimura A. (1983) Transformation of yeast cells treated with alkali cations. J Bacteriol 153: 163–168PubMedGoogle Scholar
  43. Jefferson R.A., Kavanagh T.A., Bevan M.W. (1987) GUS fusions: ß-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907PubMedGoogle Scholar
  44. John T.P. St, Davis R.W. (1979) Isolation of galactose-inducible DNA sequences from Saccharomyces cererisiae by differential plaque filter hybridisation. Cell 16: 443–452CrossRefGoogle Scholar
  45. Kearney R. Ronald P.C., Dahlbeck D., Staskawicz B. (1988) Molecular basis for evasion of plant host defence in bacterial spot disease of pepper. Nature 322: 541–543CrossRefGoogle Scholar
  46. Kistler H.C., Benny U.K. (1988) Genetic transformation of the fungal plant wilt pathogen, Fusarium oxysporum. Curr Genet 13: 145–149CrossRefGoogle Scholar
  47. Kistler H.C., Van Etten H.D. (1984) Regulation of pisatin demethylation in Nectria haematococca and its influence on pisatin tolerance and virulence. J Gen Microbiol 130: 2605–2613Google Scholar
  48. Koch E., Slusarenko A. (1990) Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2: 437 445PubMedGoogle Scholar
  49. Köller W., Allan C.R., Kolattukudy P.E. (1982a) Protection of Pisum sativum from Fusarium solani f. pisi by inhibition of cutinase with organophosphorus pesticides. Phytopathology 72: 1425–1430CrossRefGoogle Scholar
  50. Köller W., Allan C.R., Kolattukudy P.E. (1982b) Role of cutinase and cell wall degrading enzymes in infection of Pisum sativum by Fusarium solani f. sp. pisi. Physiol Plant Pathol 20: 47–60CrossRefGoogle Scholar
  51. Kolattukudy P.E. (1981) Structure, biosynthesis and biodegradation of cutin and suberin. Annu Rev Plant Physiol 32: 539–567CrossRefGoogle Scholar
  52. Kolattukudy P.E., Podila G.K., Mohan R. (1989) Molecular basis of the early events in plantfungus interaction. Genome 31: 342–349CrossRefGoogle Scholar
  53. Kronstad J.W., Leong S.A. (1989) Isolation of two alleles of the b locus of Ustilago maydis. Proc Natl Acad Sci USA 86: 978–982PubMedCrossRefGoogle Scholar
  54. Kubo Y., Tsuda M., Furasawa I., Shishiyama J. (1989) Genetic analysis of genes involved of melanin biosynthesis of Cochlioholus miyabeanus. Exp Mycol 13: 77–84CrossRefGoogle Scholar
  55. Leach J., Yoder O.C. (1983) Heterokaryon incompatibility in the plant-pathogenic fungus Cochliobols heterostrophus. J Hered 74: 149–152Google Scholar
  56. Leonhard K. (1977) Virulence, temperature optima, and competitive abilities of isolines of races T and 0 of Bipolaris maydis. Phytopathology 67: 1273–1279CrossRefGoogle Scholar
  57. Leung H., Taga M. (1988) Magnaporthe grisea the blast fungus. In: Sidhu G.S. (ed) Genetics of plant pathogenic fungi. Academic Press, London, pp 175–188 (Advances in plant pathology, vol 6)Google Scholar
  58. Leung H., Lehtinen U., Karjalainen R., Skinner D., Tooley P., Leong H., Ellingboe A. (1990) Transformation of the rice blast fungus Magnaporthe grisea to hygromycinB tolerance. CUrr Genet 17: 409–411PubMedCrossRefGoogle Scholar
  59. Lin T.S., Kolattukudy P.E. (1978) Induction of a biopolyester hydrolase (cutinase) by low levels of cutin monomers in Fusarium solani f. pisi. J Bacteriol 133: 942–951PubMedGoogle Scholar
  60. Malardier L., Daboussi M.J., Julien J., Roussel F., Scazzocchio C., Brygoo Y. (1989) Cloning of the nitrate reductase gene (nia D) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum. Gene 78: 147–156PubMedCrossRefGoogle Scholar
  61. Mangiarotti G., Chung S., Zuker C., Lodish H.F. (1981) Selection and analysis of cloned developmentally-regulated Dictyostelium discoideum genes by hybridization-competition. Nucleic Acids Res 9: 947–963PubMedCrossRefGoogle Scholar
  62. Marek E.T., Schardl C.L. Smith D.A. (1989) Molecular transformation of Fusarium solani with an antibiotic resistance marker having no fungal homology. Curr Genet 15: 421–428.PubMedCrossRefGoogle Scholar
  63. Maiti J.B., Kolattukudy P.E. (1979) Prevention of fungal infection of plants by specific inhibition of cutinase. Science 205: 507–508PubMedCrossRefGoogle Scholar
  64. McHale M.T., Roberts I.N., Talbot N.J., Oliver R.P. (1989) Expression of reverse transcriptase genes in Fulvia fulva. Mol Plant Microbe Interact 2: 165–168PubMedCrossRefGoogle Scholar
  65. Meyerowitz E.M. (1989) Arahidopsis. a useful weed. Cell 56: 263–269PubMedCrossRefGoogle Scholar
  66. Munnecke D.E. (1948) An unusual host response to certain paired monosporidial cultures of Ustilago zeae. Phytopathology 38: 19Google Scholar
  67. Nelson P.E., Toussoun T.A., Cook R.J. (1981) Fusarium diseases, biology, and taxonomy. The Pennsylvania State University Press, University ParkGoogle Scholar
  68. Oliver R.P., Roberts I.N., Harling R., Kenyon L., Punt P.J., Dingemanse M.A., van den Hondel C.A.M.J.J. (1987) Transformation of Fulvia fulva, a fungal pathogen of tomato, to hygromycin resistance. Curr Genet 12: 231–233CrossRefGoogle Scholar
  69. Orbach M.J., Porro E.B., Yanofsky C, (1986) Cloning and characterization of the gene for ß-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selection marker. Mol Cell Biol 6: 2452–2461PubMedGoogle Scholar
  70. Panaccione D.G., McKiernan M., Hanau R.M. (1988) Colletotrichum graminicola transformed with homologous and heterologous benomyl-resistance genes retains expected pathogenicity to corn. Mol Plant Microbe Interact 1: 113–120CrossRefGoogle Scholar
  71. Parsons K.A., Chumley F.G., Valent B. (1987) Genetic transformation of the fungal pathogen responsible for rice blast disease. Proc Natl Acad Sci USA 84: 4161–4165PubMedCrossRefGoogle Scholar
  72. Pitt J.J. (1979) The genus Penicillium and its telemorphic states Eupenicillium and Talaromyces. Academic Press. London Podila GK, Dickman MB, Kolattukudy PE (1988) Transcriptional activation of a cutinase gene in isolated fungal nuclei by plant cutin monomers. Science 242: 922–925Google Scholar
  73. Punt P.J., Oliver R.P., Dingemanse M.A., Pouwels P.H., Van den Hondel C.A.M.J.J. (1987) Transformation of Aspergillus based on the hygromycinB resistance marker from Esche-richia coli. Gene 56: 117–124PubMedCrossRefGoogle Scholar
  74. Rambosek J., Leach J. (1987) Recombinant DNA in filamentous fungi: progress and prospects. CRC Crit Rev Biotechnol 6: 357–393CrossRefGoogle Scholar
  75. Rinaldy A.R., Dodson M.L., Darling T.L., Lloyd R.S. (1988) Gene cloning using cDNA libraries in a differential competition hybridization strategy: application to cloning XP-A related genes. DNA 7: 563–570PubMedCrossRefGoogle Scholar
  76. Rodriguez R.J., Yoder O.C. (1987) Selectable genes for transformation of the fungal pathogen Gomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum). Gene 54: 73–81PubMedCrossRefGoogle Scholar
  77. Rowell J.B., De Vay J.E. (1954) Genetics of U stilago zeae in relation to basic problems of its pathogenicity. Phytopathology 44: 356–362Google Scholar
  78. Rowlands R.T., Turner G. (1973) Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Mol Gen Genet 126: 201–216PubMedCrossRefGoogle Scholar
  79. Saleh Y.P., Beremand M.N. (1988) Development of a transformation system for Fusarium sambucinum. J Cell Biochem [Suppl] 12C: 290Google Scholar
  80. Schäfer W., Straney D., Ciuffetti L., VanEtten H.D., Yoder O.C. (1989) One enzyme makes a fungal pathogen, but not a saprophyte, virulent on a new host plant. Science 246: 247–249PubMedCrossRefGoogle Scholar
  81. Schulz B., Banuett F., Dahl M., Schlesinger R., Schäfer W., Martin T., Herskowitz I., Kahmann R. (1990) The b alleles of Ustilago maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain related motif. Cell 60: 295–306PubMedCrossRefGoogle Scholar
  82. Shaykh M., Soliday C., Kolattukudy P.E. (1977) Proof of the production of cutinase by Fusarium solni f. pisi during penetration into its host, Pisum sativum. Plant Physiol 60: 170–172PubMedCrossRefGoogle Scholar
  83. Simpson R.B., Johnson L.J. (1990) Arabidopsis thaliana as a host for Xanthomonas campestris pv. campestris. Mol Plant Microbe Interact 3: 233–237CrossRefGoogle Scholar
  84. Snyder B.A., Nicholson R.L. (1990) Synthesis of phytoalexins in sorghum as a site-specific response to fungal ingress. Science 248: 1637–1639PubMedCrossRefGoogle Scholar
  85. Soliday C.L., Flurkey W.H., Okita T.W., Kolattukudy P.E. (1984) Cloning and structure determination of cDNA for cutinase, an enzyme involved in fungal penetration of plants. Proc Natl Acad Sci USA 81: 3939–3943PubMedCrossRefGoogle Scholar
  86. Soliday C.L., Dickman M.B., Kolattukudy P.E. (1989) Structure of the cutinase gene and detection of promotor activity in the 5′-f1anking region by fungal transformation. J Bacteriol 171: 1942–1951PubMedGoogle Scholar
  87. Thompson C.J., Movva N.R. Tizard R., Crameri R., Davies J.E., Lauwereys M., Botterman J. (1987) Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO 16: 2519–2523Google Scholar
  88. Tilburn J., Scazzocchio G., Taylor G.G., Zabicky-Zissima J.H., Lockington R.A., Davis R.W. (1983) Transformation by integration in Aspergillus nidulans. Gene 26: 205–221Google Scholar
  89. Timberlake W.E. (1980) Developmental gene regulation in Aspergillus nidulans. Dev Biol 78: 497–510.PubMedCrossRefGoogle Scholar
  90. Turgeon G.B., Garber R.C., Yoder O.C. (1985) Transformation of the fungal maize pathogen Cochliobolus heterostrophus using the Aspergillus nidulans amd S gene. Mol Gen Genet 201: 450–453CrossRefGoogle Scholar
  91. Turgeon G.B., Garber R.C., Yoder O.C. (1987) Development of a fungal transformation system based on selection of sequences with promoter activity. Mol Cell Biol 7: 3297–3305PubMedGoogle Scholar
  92. Van den Ende G., Linskens H.F. (1974) Cutinolytic enzymes in relation to pathogenesis. Annu Rev Phytopathol 12: 247–258CrossRefGoogle Scholar
  93. Van Engelenburg F., Smit R., Goosen T., van den Broek H., Tudzynski P. (1989) Transformation of Claviceps purpurea using a bleomycin resistance gene. Appl Microbiol Biotechnol 30: 364–370CrossRefGoogle Scholar
  94. VanEtten H.D., Kistler H.C. (1988) Nectria haematococca mating populations I and VI. In: Sidhu G.S. (ed) Genetics of plant pathogenic fungi. Academic Press, London, pp 189–206 (Advances in plant pathology, vol 6)Google Scholar
  95. VanEtten H.D., Mathews P.S., Tegtmeier K.J., Dietert M.F., Stein J.J. (1980) The association of pisatin tolerance and demethylation with virulence on pea in Nectria haematococca. Physiol Plant Pathol 16: 257–268CrossRefGoogle Scholar
  96. VanEtten H.D., Mathews D.E., Mathews P.S. (1989) Phytoalexin detoxification: importance for pathogenicity and practical implications. Annu Rev Phytopathol 27: 143–164PubMedCrossRefGoogle Scholar
  97. Wang J., Holden D.W., Leong S.A. (1988) Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc Natl Acad Sci USA 85: 865–869PubMedCrossRefGoogle Scholar
  98. Ward M., Wilkinson B., Turner G. (1986) Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene. Mol Gen Genet 202: 265–270PubMedCrossRefGoogle Scholar
  99. Weltring K.M., Turgeon B.G., Yoder O.C., VanEtten H.D. (1988) Isolation of a phytoalexindetoxifying gene from the plant pathogenic fungus Nectria haematococca by detecting its expression in Aspergillus nidulans. Gene 68: 335–344PubMedCrossRefGoogle Scholar
  100. Wheeler M.H., Greenblatt G.A. (1988) The inhibition of melanin biosynthetic reactions in Pyricularia oryzae by compounds that prevent rice blast disease. Exp Mycol 12: 151–160CrossRefGoogle Scholar
  101. Willison J.H.M (1981) Secretion of cell wall material in higher plants. In: Tanner W., Loewus F.A. (eds) Plant carbohydrates II. Springer, Berlin Heidelberg New York, pp 513–541 [Pierson A., Zimmermann M.H. (eds) Encyclopedia of plant physiology, vol 13B]Google Scholar
  102. Yelton M.M., Hamer J.E., Timberlake W.E. (1984) Transformation of Aspergillus nidulans by using a tryC plasmid. Proc Natl Acad Sci USA 81: 1470–1474PubMedCrossRefGoogle Scholar
  103. Yoder O.C., (1988) Cochliobolus heterostrophus, cause of southern corn leaf blight. In: Sidhu G.S. (ed) Genetics of plant pathogenic fungi. Academic Press, London, pp 93–112 (Advances in plant pathology, vol 6)Google Scholar
  104. Yoder O.C., Turgeon B.G., Schafer W., Ciuffetti L., Bohlmann H., VanEtten H.D. (1989) Molecular analysis of mating type and expression of a foreign pathogenicity gene in Cochliobolus heterostrophus. In: Nevalainen H., Penttilä M. (eds) Proceedings of the EMBO alko Workshop on Molecular Biology of Filamentous Fungi. Found Biotech Indust Ferment Res 6: 189–196Google Scholar
  105. Young R.A., Davis R.W. (1983) Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci USA 80: 1194–1198PubMedCrossRefGoogle Scholar
  106. Zimmermann C.R., Orr W.C. Leclerc R.F., Barnard E.C., Timberlake W.E. (1980) Molecular cloning and selection of genes regulated in Aspergillus development. Cell 21: 709–715PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 1992

Authors and Affiliations

  • Willi Schäfer
    • 1
  • Dietmar Stahl
    • 1
  • Enrico Mönke
    • 1
  1. 1.Institut für Genbiologische Forschung BerlinBerlin 33Federal Republic of Germany

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