Summary
Studies on the structure and function of the nonstructural proteins (NSP1-NSP5) of rotaviruses are important for dissection of the morphogenesis and replication processes of rotavirus. Above all, NSP1, the product of gene 5, has several interesting features, such as extreme sequence diversity, a highly conserved cysteine-rich region, RNA-binding activity, accumulation on the cytoskeleton, and non-random segregation in reassortment. Recently, comparable NSP1 sequence analysis has been performed on a number of rotavirus strains from various species. Furthermore, characterization of mutants with rearranged NSP1 genes has helped to elucidate the structure-function interaction of NSP1. We isolated and characterized two interesting mutants which have a large deletion including the cysteine-rich region or a nonsense codon at the early portion in the open reading frame (ORF) of the NSP1 gene. In this report, we summarize the structure and function of NSP1.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Both GW, Bellamy AR, Mitchell DB (1994) Rotavirus protein structure and function. Curr Top Microbiol Immunol 185: 67–106
Bremont M, Charpilienne A, Chabanne O, Cohen J (1987) Nucleotide sequence and expression in Escherichia coli of the gene encoding the non-structural protein NCVP2 of bovine rotavirus. Virology 161: 138–144
Brottier P, Nandi P, Bremont M, Cohen J (1992) Bovine rotavirus segment 5 protein expressed in the baculovirus system interacts with zinc and RNA. J Gen Virol 73: 1931–1938
Dunn SJ, Cross TL, Greenberg HB (1994) Comparison of the rotavirus nonstructural protein NSP1 (NS53) from different species by sequence analysis and Northern blot hybridization. Virology 203: 178–183
Eiden JJ (1994) Expression and sequence analysis of gene 7 of the IDIR agent (group B rotavirus): similarity with NS53 of group A rotavirus. Virology 199: 212–218
Estes M, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449
Gombold JL, Ramig RF (1986) Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV. J Virol 57: 110–116
Hua J, Chen X, Patton JT (1994) Deletion mapping of the rotavirus metalloprotein NS53 (NSP1): the conserved cysteine-rich region is essential for virus-specific RNA binding. J Virol 68: 3990–4000
Hua J, Mansell EA, Patton JT (1993) Comparative analysis of the rotavirus NS53 gene: conservation of basic and cysteine-rich regions in the protein and possible stem-loop structures in the RNA. Virology 196: 372–378
Hua J, Patton JT (1994) The carboxyl-half of the rotavirus protein NS53 (NSP1) is not required for virus replication. Virology 198: 567–576
Kobayashi N, Kojima K, Taniguchi K, Urasawa T, Urasawa S (1994) Genotypic diversity of reassortants between simian rotavirus SA11 and human rotaviruses having different antigenic specificities and RNA patterns. Res Virol 145: 303–311
Kojima K, Taniguchi K, Kobayashi N (1996) Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, feline, and equine rotavirus strains. Arch Virol 141: 1–12
Mitchell DB, Both GW (1990) Conservation of a potential metal binding motif despite extensive sequence diversity in the rotavirus nonstructural protein NS53. Virology 174: 618–621
Palombo EA, Bishop RF (1994) Genetic analysis of NSP1 genes of human rotaviruses isolated from neonates with symptomatic infection. J Gen Virol 75: 3635–3639
Pongsuwanna Y, Taniguchi K, Choonthanom M, Chiwakul M Susansook T, Saguanwongse S, Jayavasu S, Urasawa S (1989) Subgroup and serotype distributions of human, bovine, and porcine rotavirus in Thailand. J Clin Microbiol 27: 1956–1960
Pongsuwanna Y, Taniguchi K, Wakasugi F, Sutivijit Y, Chiwakul M, Warachit P, Jayavasu C, Urasawa S (1993) Distinct yearly change of serotype distribution of human rotavirus in Thailand as determined by ELISA and PCR. Epidemiol Infect 111: 407–412
Ramig RF, Ward RL (1991) Genomic segment reassortment in rotaviruses and other Reoviridae. Adv Virus Res 39: 163–207
Taniguchi K, Kojima K, Urasawa S (1996) Non-defective mutants with NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155. J Virol 70: 4125–4130
Taniguchi K, Urasawa T, Pongsuwanna Y, Choonthanom M, Jayavasu C, Urasawa S (1991) Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol 72: 2929–2937
Taniguchi K, Urasawa T, Urasawa S (1993) Independent segregation of the VP4 and the VP7 genes in bovine rotaviruses as confirmed by VP4 sequence analysis of G8 and G10 bovine rotavirus strains. J Gen Virol 74: 1215–1221
Tian Y, Tarlow O, Ballard A, Desselberger U, McCrae MA (1994) Genomic concatemerization/deletion in rotaviruses: a new mechanism for generating rapid genetic change of potential epidemiological importance. J Virol 67: 6625–6632
Xu L, Tian Y, Tarlow O, Harbour D, McCrae MA (1994) Molecular biology of rotaviruses, IX. Conservation and divergence in genome segment 5. J Gen Virol 75: 3413–3421
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Wien
About this paper
Cite this paper
Taniguchi, K., Kojima, K., Kobayashi, N., Urasawa, T., Urasawa, S. (1996). Structure and function of rotavirus NSP1. In: Chiba, S., Estes, M.K., Nakata, S., Calisher, C.H. (eds) Viral Gastroenteritis. Archives of Virology, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6553-9_6
Download citation
DOI: https://doi.org/10.1007/978-3-7091-6553-9_6
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82875-5
Online ISBN: 978-3-7091-6553-9
eBook Packages: Springer Book Archive